RESUMEN

Group A Streptococcus pyogenes (GAS) is a leading human pathogen that produces a powerful cytolytic bacteriocin known as streptolysin S (SLS). We have developed a bioengineering strategy to successfully reconstitute SLS activity using heterologous expression in laboratory strains of Escherichia coli. Our E. coli-based heterologous expression system will allow more detailed studies into the biosynthesis of other bacteriocin compounds and the production of these natural products in much greater yield.

Asunto(s)

Proteínas Bacterianas/biosíntesis , Escherichia coli/genética , Ingeniería Genética/métodos , Estreptolisinas/biosíntesis , Secuencia de Aminoácidos , Animales , Proteínas Bacterianas/química , Proteínas Bacterianas/genética , Proteínas Bacterianas/toxicidad , Hemólisis/efectos de los fármacos , Familia de Multigenes/genética , Ovinos , Estreptolisinas/química , Estreptolisinas/genética , Estreptolisinas/toxicidad