Asunto(s)

Pruebas Inmunológicas de Citotoxicidad , Proteínas Luminiscentes/genética , Citometría de Flujo , Vectores Genéticos , Proteínas Fluorescentes Verdes , Humanos , Células Asesinas Naturales/inmunología , Proteínas Recombinantes/genética , Transducción Genética , Células Tumorales Cultivadas

RESUMEN

The pathogenicity of chronic gastroduodenal diseases is very often related to Helicobacter pylori infections. Most H. pylori strains carry the cagA gene encoding an immunodominant 120- to 128-kDa protein which is considered a virulence marker. The majority of CagA-positive H. pylori isolates also produce a 95-kDa protein cytotoxin (VacA) causing vacuolation and degradation of mammalian cells. In our previous study we have shown that live H. pylori bacteria and their sonicates inhibit PHA-driven proliferation of human T lymphocytes. The H. pylori CagA and VacA proteins were suspected of a paralyzing effect of H. pylori on T cell proliferation. In this report, by using isogenic H. pylori mutant strains defective in CagA and VacA proteins, we determined that CagA is responsible for the inhibition of PHA-induced proliferation of T cells.

Asunto(s)

Antígenos Bacterianos/inmunología , Proteínas Bacterianas/inmunología , Helicobacter pylori/inmunología , Linfocitos T/inmunología , Antígenos Bacterianos/genética , Proteínas Bacterianas/genética , División Celular/efectos de los fármacos , Células Cultivadas , Citotoxinas/genética , Citotoxinas/inmunología , Helicobacter pylori/genética , Helicobacter pylori/fisiología , Humanos , Fitohemaglutininas/farmacología , Linfocitos T/citología , Linfocitos T/efectos de los fármacos , Linfocitos T/microbiología