RESUMEN
The use of multivariate pattern recognition for the analysis of neural representations encoded in fMRI data has become a significant research topic, with wide applications in neuroscience and psychology. A popular approach is to learn a mapping from the data to the observed behavior. However, identifying the instantaneous cognitive state without reference to external conditions is a relatively unexplored problem and could provide important insights into mental processes. In this paper, we present preliminary but promising results from the application of an unsupervised learning technique to identify distinct brain states. The temporal ordering of the states were seen to be synchronized with the experimental conditions, while the spatial distribution of activity in a state conformed with the expected functional recruitment.
Asunto(s)
Inteligencia Artificial , Mapeo Encefálico/métodos , Encéfalo/fisiología , Cognición/fisiología , Potenciales Evocados/fisiología , Imagen por Resonancia Magnética/métodos , Reconocimiento de Normas Patrones Automatizadas/métodos , Algoritmos , Humanos , Aumento de la Imagen/métodos , Interpretación de Imagen Asistida por Computador/métodos , Reproducibilidad de los Resultados , Sensibilidad y EspecificidadRESUMEN
We propose an anisotropic diffusion method to denoise and aid the reconstruction of planar objects in three-dimensional images. The contribution of this paper is the development of a planarity function characterizing plate-like structures using an image Hessian's eigensystem. We then construct a diffusion tensor for anisotropically smoothing plates and satisfying necessary scale-space properties. Our method finds applications in improving the fidelity of highly noisy cell membrane images from confocal microscopy. In dense cellular regions, cell membranes assume linear shapes (planar) between neighbors. The imaging process makes cell membranes appear as diffuse structures owing to the non-uniform fluorescent marker distribution, point-spread function of the optics, and anisotropic voxel resolution which make automatic cell segmentation difficult. We apply diffusion filtering to identify and enhance membranes. We demonstrate the use of our methods on 3D cell membrane images of a zebrafish embryo acquired using fluorescent microscopy and quantify the improvement in image quality.