RESUMEN

Poly(DL-lactide-co-glycolide) (PDLLGA) and poly(L-lactide-co-glycolide) (PLLGA) copolymers were prepared by bulk ring opening polymerization of lactide and glycolide and characterized by GPC, FTIR, 1H NMR and DSC. Copolymers with different molar masses at a constant lactide/glycolide ratio were used for preparation of bovine serum albumin (BSA)-loaded microparticles by the double emulsion w/o/w method. The influence of the copolymer molar mass and composition on the microparticle morphology, size, yield, degradation rate, BSA-loading efficiency and BSA release profile were studied. For microparticles prepared from PDLLGA copolymers, a biphasic profile for BSA release was found and for those made from PLLGA copolymers the release profile was typically triphasic; both of them were characterized by high initial burst release. Possible reasons for such behavior are discussed.

Asunto(s)

Preparaciones de Acción Retardada/síntesis química , Preparaciones de Acción Retardada/farmacocinética , Ácido Láctico/síntesis química , Ácido Láctico/farmacocinética , Tamaño de la Partícula , Ácido Poliglicólico/síntesis química , Ácido Poliglicólico/farmacocinética , Polímeros/síntesis química , Polímeros/farmacocinética , Animales , Bovinos , Química Farmacéutica/métodos , Portadores de Fármacos/síntesis química , Portadores de Fármacos/farmacocinética , Emulsiones , Glicolatos/síntesis química , Glicolatos/farmacocinética , Ácido Láctico/clasificación , Ácido Poliglicólico/clasificación , Copolímero de Ácido Poliláctico-Ácido Poliglicólico , Polímeros/clasificación , Albúmina Sérica Bovina/química , Albúmina Sérica Bovina/farmacocinética , Tecnología Farmacéutica/métodos