RESUMEN
BACKGROUND AND OBJECTIVES: The worldwide increase in antimicrobial resistance in Neisseria gonorrhoeae prompted the authors to evaluate the status and course of resistance in gonococci in Finland. GOALS: The minimal inhibitory concentrations (MIC) of penicillin, tetracycline, spectinomycin, ciprofloxacin, ceftriaxone, and cefixime were tested for 337 consecutive clinical N. gonorrhoeae isolates collected in 19 Finnish microbiology laboratories in 1993. STUDY DESIGN: The results were compared with data obtained in three Finnish laboratories in 1986 and contrasted with the development of the incidence of gonorrhea and the prevalence of penicillinase-producing N. gonorrhoeae (PPNG) in Finland, 1976 to 1995. The number of strains with an elevated MIC to ciprofloxacin was assessed by questionnaire. RESULTS: A decrease, from more than 50% in 1986 to 20% in 1993, of strains susceptible to penicillin and tetracycline was observed. The prevalence of PPNG increased from 0% (1976) to 5.7% (1995). In 1995, two strains with a ciprofloxacin MIC of > or = 32 micrograms/ml were reported. No resistance to ceftriaxone or spectinomycin was detected. CONCLUSIONS: In spite of the rarity of gonorrhea and the availability of efficient antimicrobials in Finland, monitoring of the antimicrobial resistance of N. gonorrhoeae remains important.
Asunto(s)
Neisseria gonorrhoeae/efectos de los fármacos , Cefalosporinas/farmacología , Ciprofloxacina/farmacología , Farmacorresistencia Microbiana , Gonorrea/tratamiento farmacológico , Humanos , Pruebas de Sensibilidad Microbiana , Factores de TiempoAsunto(s)
Brotes de Enfermedades , Enfermedades Transmitidas por los Alimentos/epidemiología , Enfermedades Transmitidas por los Alimentos/microbiología , Femenino , Finlandia/epidemiología , Enfermedades Transmitidas por los Alimentos/diagnóstico , Enfermedades Transmitidas por los Alimentos/terapia , Guías como Asunto , Humanos , Incidencia , Masculino , Factores de RiesgoRESUMEN
A selection of 100 Corynebacterium diphtheriae isolates from asymptomatic carriers and clinical cases from five regions in northwestern Russia were examined. Six additional isolates from patients in Finland and Estonia with epidemiological links to Russia were also examined. All isolates were characterized by biotyping, toxigenicity testing, ribotyping, and pulsed-field gel electrophoresis (PFGE). Hybridization of genomic DNA digested with BstEII revealed five ribotype patterns among the biotype gravis isolates (G1 through G5) and two patterns among the biotype mitis isolates (M1 and M2). PFGE using SfiI was not able to distinguish between ribotypes G1, G2, and G4. The predominant ribotype pattern, G1, found in cases of disease in all the areas studied, appears to be disseminating, in view of the isolates received from imported cases in Finland and Estonia. Among the 106 isolates examined, 68 produced pattern G1 and 24 produced pattern M1. Most of the M1 isolates were from the Leningrad Oblast region. Distinct ribotypes such as G2, G3, G4, G5, and M2 could represent endemic disease.
Asunto(s)
Corynebacterium diphtheriae/genética , Difteria/epidemiología , Técnicas de Tipificación Bacteriana , Portador Sano/epidemiología , Portador Sano/microbiología , Corynebacterium diphtheriae/clasificación , Corynebacterium diphtheriae/aislamiento & purificación , ADN Bacteriano/genética , ADN Bacteriano/aislamiento & purificación , Difteria/microbiología , Electroforesis en Gel de Campo Pulsado , Estonia/epidemiología , Finlandia/epidemiología , Humanos , Epidemiología Molecular , ARN Ribosómico/genética , Federación de Rusia/epidemiologíaRESUMEN
The in-vitro activities of ten antibiotics against 83 toxigenic strains of Corynebacterium diphtheriae recently isolated in northwestern Russia and surrounding areas were determined by an agar dilution method. All of the strains were susceptible to erythromycin, penicillin, ampicillin, cefuroxime, chloramphenicol, ciprofloxacin, gentamicin and tetracycline. Trimethoprim and rifampicin were each active against 81 isolates, the two strains resistant to the latter agent having been isolated from two members of the same family.
Asunto(s)
Antibacterianos/farmacología , Corynebacterium diphtheriae/efectos de los fármacos , Países Bálticos , Ciprofloxacina/farmacología , Eritromicina/farmacología , Humanos , Pruebas de Sensibilidad Microbiana , Rifampin/farmacología , Federación de RusiaRESUMEN
Serotyping is widely used for epidemiologic investigation of group A streptococci (GAS). To evaluate molecular typing methods of GAS, restriction endonuclease analysis (REA) of genomic DNA and analysis of DNA restriction fragment length polymorphism of rRNA genes (ribotyping) were used in parallel with serotyping. The genomic DNA of 239 epidemiologically unrelated GAS isolates from human invasive infections was digested with HindIII restriction enzyme. Both REA and ribotyping differentiated subclasses within serotypes. However, they did not consistently differentiate between isolates of different serotypes. Ribotyping was less discriminatory than REA. Within the T1M1 serotype, often associated with invasive GAS infections, 92% of the REA patterns were identical, suggesting a common origin for these isolates. Most other serotypes studied were more heterogenic. Among 32 isolates nontypeable by serotyping, 11 distinct REA patterns and 5 ribotypes were identified. REA and ribotyping are useful supplementary tools for classification of GAS and can add to the discriminatory power of serotyping.
Asunto(s)
Estudios Seroepidemiológicos , Serotipificación/métodos , Infecciones Estreptocócicas/epidemiología , Streptococcus pyogenes/clasificación , Desoxirribonucleasa HindIII/metabolismo , Farmacorresistencia Microbiana , Eritromicina/farmacología , Humanos , Polimorfismo de Longitud del Fragmento de Restricción , Prohibitinas , Infecciones Estreptocócicas/microbiología , Streptococcus pyogenes/efectos de los fármacosAsunto(s)
Difteria/transmisión , Viaje , Adulto , Difteria/inmunología , Toxoide Diftérico , Finlandia , Humanos , Inmunización Secundaria , Masculino , Federación de RusiaRESUMEN
Seventy-four cases of systemic listeriosis occurring from 1971 to 1989 in the greater Helsinki area in Finland are reviewed with a special interest in the effect of preceding immunosuppressive therapy on the clinical presentation. Of these patients, 66% had an underlying disease, most commonly malignancy, diabetes mellitus, or renal transplantation, and 43% had received immunosuppressive therapy within 1 week before onset of listeriosis. Bacteremia and central nervous system infections (both in 43% of cases) were the most common clinical entities. The percentage of patients with meningitis was not greater among immunosuppressed patients (13/32, 41%) than among patients with underlying diseases not treated with immunosuppressive agents (9/16, 56%) or among previously healthy nonpregnant hosts (7/11, 64%). Immunosuppressed patients did not die more frequently than did those with underlying diseases not treated with immunosuppressive therapy (case fatality rate, 29% vs. 38%, respectively). However, all previously healthy non-neonatal patients survived, whereas 32% (15/47) of those with any kind of underlying disease succumbed.
Asunto(s)
Bacteriemia/etiología , Terapia de Inmunosupresión , Listeriosis/etiología , Meningitis por Listeria/etiología , Adolescente , Adulto , Anciano , Alanina Transaminasa/sangre , Aminoglicósidos , Ampicilina/uso terapéutico , Antibacterianos/uso terapéutico , Aspartato Aminotransferasas/sangre , Bacteriemia/tratamiento farmacológico , Bacteriemia/inmunología , Sedimentación Sanguínea , Niño , Preescolar , Complicaciones de la Diabetes , Quimioterapia Combinada/uso terapéutico , Femenino , Gastroenteritis/tratamiento farmacológico , Gastroenteritis/etiología , Gastroenteritis/inmunología , Humanos , Lactante , Recién Nacido , Trasplante de Riñón , Listeriosis/tratamiento farmacológico , Listeriosis/inmunología , Meningitis por Listeria/tratamiento farmacológico , Meningitis por Listeria/inmunología , Persona de Mediana Edad , Neoplasias/complicaciones , Penicilina G/uso terapéutico , Piperacilina/uso terapéutico , Embarazo , Complicaciones Infecciosas del Embarazo/tratamiento farmacológico , Complicaciones Infecciosas del Embarazo/etiología , Complicaciones Infecciosas del Embarazo/inmunología , Resultado del TratamientoRESUMEN
BACKGROUND: The use of erythromycin in Finland nearly tripled from 1979 to 1989. In 1988, we observed an unusually high frequency of resistance to erythromycin in group A streptococci in one geographic region. Because routine testing does not detect the sensitivity of these organisms to antibiotics, we initiated a national study to evaluate the extent of this resistance. METHODS: We studied 272 isolates of group A streptococci obtained from blood cultures from 1988 through 1990. In 1990 we collected from six regional laboratories 3087 consecutive isolates from throat swabs and 1349 isolates from pus samples. Resistance was indicated by growth on blood agar containing 2 micrograms of erythromycin per milliliter after incubation in 5 percent carbon dioxide. We also evaluated the clinical importance of erythromycin resistance in a retrospective study of consecutive patients with pharyngitis. RESULTS: The frequency of resistance to erythromycin in group A streptococci from blood cultures increased from 4 percent in 1988 to 24 percent in 1990. From January to December 1990, the frequency of resistance in isolates from throat swabs increased from 7 percent to 20 percent, and resistance in isolates from pus increased from 11 percent to 31 percent. In four communities within 50 km of each other, the frequency of erythromycin resistance ranged from 2 to 5 percent to 26 to 44 percent. Several distinct DNA restriction profiles and serotypes were found among resistant isolates from the same area, suggesting a multiclonal origin. The treatment of pharyngitis with erythromycin failed in 9 of 19 patients infected with erythromycin-resistant group A streptococci, as compared with 1 of 26 patients with erythromycin-susceptible isolates (47 percent vs. 4 percent, P = 0.008). CONCLUSIONS: In Finland since 1988 there has been a rapid and substantial increase in resistance to erythromycin in group A streptococci. The extent of this resistance is particularly serious since there are only a few alternative antibiotics available for peroral treatment of group A streptococcal infections.
Asunto(s)
Eritromicina/farmacología , Streptococcus pyogenes/efectos de los fármacos , Sangre/microbiología , Farmacorresistencia Microbiana , Eritromicina/uso terapéutico , Finlandia , Humanos , Faringitis/tratamiento farmacológico , Faringitis/microbiología , Faringe/microbiología , Estudios Retrospectivos , Infecciones Estreptocócicas/tratamiento farmacológico , Streptococcus pyogenes/genética , Streptococcus pyogenes/aislamiento & purificación , Supuración/microbiologíaRESUMEN
We describe an outbreak of Salmonella typhimurium infection, caused by contaminated chocolate produced by one Norwegian company, which occurred in Norway and Finland in 1987. A total of 349 bacteriologically verified cases were recorded in Norway, and 12 cases were recorded in Finland. There was a predominance of young children among the patients (median age, 6 years), many of whom developed acute hemorrhagic diarrhea. The outbreak strain exhibited a rare phage lysis pattern and a characteristic plasmid profile lacking the 60-MDa virulence-associated plasmid. DNA hybridization failed to demonstrate any DNA sequence homology between the outbreak strain and the virulence plasmid. The outbreak strain was nonlethal for orally infected mice. The finding of only less than or equal to 10 S. typhimurium cells per 100 g of chocolate in about 90% of the positive samples obtained from retail outlets suggested that an inoculum of fewer than 10 organisms may have been sufficient to cause symptomatic disease.
Asunto(s)
Brotes de Enfermedades , Intoxicación Alimentaria por Salmonella/epidemiología , Salmonella typhimurium , Cacao/efectos adversos , Finlandia/epidemiología , Humanos , Noruega/epidemiología , Plásmidos , Salmonella typhimurium/genética , Virulencia/genéticaRESUMEN
Trimethoprim (TMP) resistance among Shigella species isolated from Finnish travelers increased from 3.0% in 1975-1982 to 42.0%-43.8% in 1987-1988. Of the 317 TMP-resistant Shigella isolates identified during 1975-1988, 175 (55%) collected in 1985-1987 and in 1988 were tested further. Almost all (98%) were highly resistant to TMP, suggesting a plasmid-mediated origin. The type I dihydrofolate reductase (DHFR) gene was detected in 85% of the isolates studied. Twenty-three percent of the type I DHFR-positive isolates failed to hybridize with a probe detecting only Tn7-derived sequences, suggesting that the type I DHFR gene may occur independently of transposon Tn7. Four of the five Shigella species isolated from travelers to Sri Lanka hybridized with the probe for type V DHFR gene, implying a local distribution of the type V DHFR gene. The type II and type III DHFR genes were not found among the isolates studied. Only 12% of the TMP-resistant Shigella isolates failed to hybridize with any of the DHFR gene probes used.
Asunto(s)
Disentería Bacilar/microbiología , Shigella/efectos de los fármacos , Resistencia al Trimetoprim , Sondas de ADN , Finlandia , Humanos , Hibridación de Ácido Nucleico , Plásmidos , Shigella/genética , Shigella flexneri/efectos de los fármacos , Shigella flexneri/genética , Shigella sonnei/efectos de los fármacos , Shigella sonnei/genética , Tetrahidrofolato Deshidrogenasa/genética , Viaje , Resistencia al Trimetoprim/genéticaRESUMEN
A waterborne Campylobacter jejuni outbreak in the Rheumatism Foundation Hospital in Heinola, Finland, in November-December 1986 is described. 32 patients and 62 members of the staff developed gastrointestinal symptoms. C. jejuni heat-stable serotype 45 was isolated from the faeces of 32 enteritis patients and from none of the controls. No other enteropathogens were found. Positive serological responses to C. jejuni acid extract antigen were detected by enzyme immunoassay in 34% of the symptomatic hospital patients, in 40% of the symptomatic staff members, and in 10% of the controls. The clinical course of the illness was mostly mild and self-limited. No striking progress in the arthritis symptoms of the patients was found after the outbreak. The hospital has its own water supply. C. jejuni of the same serotype as the epidemic strain was isolated from the water of the pipeline system. After a careful examination some aged components of the waterworks were found to be responsible for leaks that resulted in the contamination of the water.
Asunto(s)
Infecciones por Campylobacter/epidemiología , Infección Hospitalaria/epidemiología , Brotes de Enfermedades , Enfermedades Reumáticas/complicaciones , Microbiología del Agua , Adulto , Anciano , Anticuerpos Antibacterianos/análisis , Infecciones por Campylobacter/etiología , Campylobacter fetus/inmunología , Campylobacter fetus/aislamiento & purificación , Infección Hospitalaria/etiología , Diarrea/epidemiología , Diarrea/etiología , Heces/microbiología , Finlandia/epidemiología , Estudios de Seguimiento , Humanos , Inmunoglobulinas/análisis , Persona de Mediana Edad , Estudios Retrospectivos , Abastecimiento de AguaRESUMEN
Plasmid profile analysis, restriction endonuclease analysis, and multilocus enzyme electrophoresis were used in conjunction with serotyping, bacteriophage typing, and biochemical fingerprinting to trace epidemiologically related isolates of Salmonella typhimurium from an outbreak caused by contaminated chocolate products in Norway and Finland. To evaluate the efficiency of the epidemiological marker methods, isolates from the outbreak were compared with five groups of control isolates not known to be associated with the outbreak. Both plasmid profile analysis and phage typing provided further discrimination over that produced by serotyping and biochemical fingerprinting. Plasmid profile analysis and phage typing were equally reliable in differentiating the outbreak isolates from the epidemiologically unrelated controls and were significantly more effective than multilocus enzyme electrophoresis and restriction enzyme analysis of total DNA. The greatest differentiation was achieved when plasmid profile analysis and phage typing were combined to complement serotyping and biochemical fingerprinting. However, none of the methods employed, including restriction enzyme analysis of plasmid DNA, were able to distinguish the outbreak isolates from five isolates recovered in Norway and Finland over a period of years from dead passerine birds and a calf.
Asunto(s)
Cacao , Brotes de Enfermedades , Plantas Comestibles , Intoxicación Alimentaria por Salmonella/epidemiología , Salmonella typhimurium/clasificación , Animales , Técnicas de Tipificación Bacteriana , Tipificación de Bacteriófagos , Enfermedades de las Aves/microbiología , Aves , ADN Bacteriano/análisis , Finlandia , Contaminación de Alimentos , Humanos , Noruega , Fenotipo , Plásmidos , Mapeo Restrictivo , Intoxicación Alimentaria por Salmonella/microbiología , Salmonelosis Animal/microbiología , Salmonella typhimurium/enzimología , Salmonella typhimurium/genética , SerotipificaciónAsunto(s)
Infecciones por Campylobacter/epidemiología , Brotes de Enfermedades/epidemiología , Adolescente , Adulto , Infecciones por Campylobacter/microbiología , Campylobacter fetus/aislamiento & purificación , Heces/microbiología , Femenino , Finlandia , Humanos , Masculino , Microbiología del Agua , Abastecimiento de AguaRESUMEN
The efficacy of a narrow-spectrum (benzyl penicillin) versus broad-spectrum (clindamycin + gentamicin) preoperative antimicrobial prophylaxis was studied in a series of 147 consecutive patients undergoing Caesarean section at the State Maternity Hospital, Helsinki, Finland. Both regimens proved effective in reducing postoperative endometritis: from 33% (19/57 cases) to 6.5% (3/46 cases) in the penicillin treated group, and to 9.5% (4/42 cases) in the clindamycin + gentamicin treated group. The reduction in the incidence of endometritis was not reflected in the duration of hospital stay, which was 7.7 days in the untreated group, 7.8 days in the penicillin treated group, and 7.6 days in the clindamycin + gentamicin treated group. No significant differences between the groups were detected in the incidence of wound infections.