RESUMEN
In this study, two metal Schiff base complexes (cobalt(II) and manganese(II), were synthesized and characterized by 1H-NMR and FT-IR analyses. The in vitro binding behavior of this complexes with ß-Casein (ß-CN) was investigated by using biophysical techniques. For evaluation the thermodynamics parameters of interaction between Schiff base complexes and ß-CN,the fluorescence data at different temperatures were done. The results showed that the intrinsic fluorescence of the ß-CN was quenched by increasing the complexes through the dynamic quenching mechanism. Also, these complexes demonstrated a considrable binding affinity to ß-CN and the process is mainly entropy driven. Fluorescence resonance energy transfer was used in order to estimating the distance between donor (ß-CN) and acceptor (complex). Results demonstrated that the sequence of tendency of the complexes to ß-CN was as follows: Mn-Schiff base complex > Co-Schiff base complex. All these experimental results propose that ß-CN might act as carrier protein for Co(II) and Mn (II) complexes to deliver it to the target molecules.