RESUMEN
OBJECTIVE: To assess presence of HPV infection and identification of the most common HPV types in patients with abnormal cytology based on the Bethesda system (atypical squamous cells). MATERIAL AND METHOD: 81 women with abnormal cytology based on the Bethesda system (atypical squamous cells) were qualified for the study. Material was taken from the cervical canal, the vaginal portion of the cervix and the vagina onto a liquid medium to detect HPV DNA and genotyping of 19 most common oncogenic types of high and medium risk was performed with the Papillomastrip method and for HPV types 6 and 11 with the PCR method. RESULTS: HPV was detected in 53 out of 81 examined women, which accounted for 66%. The most common HPV types were: 6/11 - 23 cases (43% of women with infection), 16 - 23 cases (43% of women with infection), 18 and 33 with 9 cases each (17% of women with infection). Coexistence of 6/11 with 16 or 18 - 13 concerned 15 patients (28% of women with infection) and presence of HPV 16 or 18 was detected in 28 cases (53% of women with infection). Positive HPV type contained in the quadrivalent vaccine against human papillomavirus 6/11 and 16 or 18 was detected in 38 patients (72% of women with infection). 40% of HPV positive women were infected with only one type of the virus, 26%--with two types and 23% with three types. CONCLUSION: In 81 women with abnormal cytology based on the Bethesda system (atypical squamous cells) within 66% of HPV positive results the most common were type 6/11 (of low oncogenic potential but responsible for anogenital warts) and type 16 of high oncogenic potential.
Asunto(s)
Células Escamosas Atípicas del Cuello del Útero , Papillomavirus Humano 16/aislamiento & purificación , Papillomavirus Humano 18/aislamiento & purificación , Displasia del Cuello del Útero/virología , Neoplasias del Cuello Uterino/virología , Adulto , Cuello del Útero/patología , Cuello del Útero/virología , Femenino , Técnicas de Genotipaje , Humanos , Persona de Mediana Edad , Neoplasias del Cuello Uterino/diagnóstico , Neoplasias del Cuello Uterino/patología , Frotis Vaginal/métodos , Displasia del Cuello del Útero/diagnóstico , Displasia del Cuello del Útero/patologíaRESUMEN
UNLABELLED: The genetic factor remains the most frequent cause of spontaneous abortions. Examination of the fetal tissue from spontaneous miscarriages shows that 75% of them were caused by abnormal karyotype. Other reasons, albeit rare, included submicroscopic genomic rearrangements, monogenic diseases, and polygenic inheritance disorders of the embryo. OBJECTIVE: The aim of the study was to analyze the incidence of chromosomal aberrations in material from the miscarriage. MATERIAL AND METHODS: The study included 47 samples of miscarriage material from 47 women. Fluorescent hybridization in-situ (FISH) was used for genetic examination. RESULTS: Chromosomal abnormalities were diagnosed in 72% of the samples, with trisomy 21 (25.5%), trisomy 16 (17%), and trisomy 18 (12.8%) as the most common. An abnormal number of copies of chromosome 18, 21, 22, indicating the coexistence of trisomy 18, 21, 22, was detected in 1 patient. It was another miscarriage in case of 14 subjects (29.8%). CONCLUSIONS: Chromosomal aberrations were diagnosed in the majority of fetal tissue samples from spontaneous miscarriages. More than one chromosomal aberration in a single embryo is an extremely rare occurrence. Miscarriage due to chromosomal aberrations occurred in the vast majority of women > 35 years of age.
Asunto(s)
Aborto Espontáneo/genética , Aberraciones Cromosómicas/estadística & datos numéricos , Cromosomas Humanos 13-15/genética , Cromosomas Humanos 16-18/genética , Cromosomas Humanos 21-22 e Y/genética , Adulto , Femenino , Humanos , Hibridación Fluorescente in Situ , Edad Materna , Reacción en Cadena de la Polimerasa , Embarazo , Trisomía/genéticaRESUMEN
A diagnostic procedure in the medical treatment of middle hydrothorax at haemodynamically efficient fetus has been presented in the research. Container of the liquid in the fetal thorax has been observed since the eleventh week of pregnancy. Infection by TORCH viruses has been excluded and typical 46,XX female karyotype has been found in the cytogenic test. A liquid in the pericardial sack (without any sings of the fetal heart defect) was observed in the ECHO examination from 23 to 37 week of pregnancy. Cesarean section was made after 37 weeks of pregnancy and the new-born baby was found to be in good condition, with body mass of 3130g and 9 points in the Apgar scale. The child cardiologist did not diagnose any heart disorders. Awaiting attitude, while monitoring the state of the fetus, seems to be the right course of action in cases of haemodynamically efficient fetus with isolated hydrothorax.