RESUMEN
The European catfish Silurus glanis is attracting growing interest as an object of fisheries and aquaculture, which is reinforced by the expansion of its natural range under climate change. Shaping the effective exploitation strategy for this valuable species requires detailed knowledge of its biology, including feeding and digestion processes, especially near the natural limits of the species range. Meanwhile, the digestion physiology of the European catfish remains poorly explored, including the activity of major digestive enzymes and the possible effect of intestinal parasites on this activity. In this regard, the activity of proteinases and α-amylase in the intestinal mucosa of the catfish was studied. Adult catfish were collected in the Rybinsk reservoir (Upper Volga) located close to the northern limit of the species range. It was shown that all subclasses of intestinal digestive proteinases, including serine proteinases, metalloproteases and cysteine (thiol) proteinases, function in the gut mucosa of the catfish. The mucosal levels of total proteolytic activity depended on fish size, in contrast to those of trypsin, chymotrypsin and α-amylase. The level of chymotrypsin activity was significantly higher than that of trypsin activity. It was also found that the incubation medium and extract of the cestodes Silurotaenia siluri parasitizing the catfish gut had a significant inhibitory effect on the activity of serine proteases (trypsin and chymotrypsin) operating in the intestines of the host fish.
Asunto(s)
Bagres , Cestodos , Parásitos , Animales , Tripsina , Quimotripsina , Mucosa Intestinal , Péptido Hidrolasas , alfa-AmilasasRESUMEN
Currently, little is known about inhibitory substances enabling tapeworms to settle in fish intestines thereby avoiding proteolysis. Contrary to previous studies with certain host-parasite pairs, this research compares the inhibitory capacities in three tapeworm species of the same genus Proteocephalus from four different fishes (P. torulosus from dace and zope, P. sagittus from stone loach and P. cernuae from ruffe). The tapeworm extracts studied significantly reduced the activity of commercial trypsin (although to a lesser degree than the synthetic inhibitor of serine proteinases PMSF), displaying clear inter-specific variation in worms' inhibitory ability. We also measured the proteolytic activity of the host intestinal mucosa exposed to tapeworm extracts which served as inhibitors. Based on per cent inhibition values, all tapeworm extracts significantly suppressed the mucosal proteolytic activity, with marked differences between certain host-parasite pairs. SDS-PAGE electrophoresis of the incubation media and extracts detected in each tapeworm species 20-36 protein bands with apparent molecular weights from 10-12 to 312.5 kDa, mostly below 50 kDa. The incubation medium and extract of each parasite shared one to six bands ranging from 12 to 35 kDa, depending on its species, with only four bands common for two or more species. The band profiles suggest that in various Proteocephalus species inhibitory capacities against host proteinases can be ensured by different proteins.
Asunto(s)
Cestodos , Infecciones por Cestodos , Cyprinidae , Enfermedades de los Peces , Animales , Cestodos/metabolismo , Infecciones por Cestodos/parasitología , Infecciones por Cestodos/veterinaria , Cyprinidae/parasitología , Enfermedades de los Peces/parasitología , Péptido Hidrolasas/metabolismoRESUMEN
The mechanisms enabling fish tapeworms to avoid proteolytic attacks by digestive enzymes of their fish host have been studied in less detail compared with mammalian cestodes. This study aimed to assess the inhibitory ability towards trypsin and chymotrypsin in Eubothrium rugosum, an intestinal parasite of burbot Lota lota, and establish its localization in the tapeworm. To this end, the worms were treated with Triton X-100 followed by differential centrifugation to isolate the tegumental brush border membrane. The protease inhibitory abilities of the worms were mostly determined by their excretory/secretory products released into the incubation medium. These inhibitory abilities proved to be linked mainly with the brush border fractions. Notably, the per cent inhibition of both studied digestive enzymes (trypsin and chymotrypsin) hardly depended on the duration of the parasite exposure in the incubation medium, probably due to intermittent glycocalyx renewal. Improved knowledge on functions of the excretory/secretory proteins produced by fish tapeworms may contribute to a better understanding of host-parasite relations and development of new approaches to the treatment and prevention of diseases caused by pathogenic helminths.
Asunto(s)
Cestodos/metabolismo , Inhibidores de Proteasas/metabolismo , Animales , Infecciones por Cestodos/enzimología , Infecciones por Cestodos/veterinaria , Quimotripsina/antagonistas & inhibidores , Enfermedades de los Peces/parasitología , Peces/parasitología , Gadiformes , Interacciones Huésped-Parásitos , Inhibidores de TripsinaRESUMEN
Using the approach of sequencing the V3-V4 region of the 16S rRNA gene, we have analysed the bacterial diversity associated with the distinct compartments of the gastrointestinal tract of perch (Perca fluviatilis) and cestodes (Proteocephalus sp.) parasitizing their digestive tract. The dominant microbiota associated with cestodes (Proteocephalus sp.) was represented by bacteria from the genera Serratia, Pseudomonas and Mycoplasma. By comparing the associated microbiota of perch and cestodes, a clear difference in bacterial composition and diversity was revealed between the community from the stomach content and other parts of the gastrointestinal tract of fish. Microbiota associated with cestodes was not significantly different in comparison with microbiota of different subcompartments of perch (mucosa and content of intestine and pyloric caeca) (ADONIS, p > .05) excluding microbiota of stomach content (ADONIS, p ≤ .05). PICRUSt-based functional assessments of the microbial communities of perch and cestodes indicated that they mainly linked in terms of metabolism and environmental information processing and could play an important role in the nutrition and health of host.
Asunto(s)
Infecciones por Cestodos/veterinaria , Enfermedades de los Peces/epidemiología , Enfermedades Gastrointestinales/veterinaria , Microbioma Gastrointestinal , Percas , Animales , Infecciones por Cestodos/epidemiología , Infecciones por Cestodos/parasitología , Femenino , Enfermedades de los Peces/parasitología , Enfermedades Gastrointestinales/epidemiología , Enfermedades Gastrointestinales/parasitología , Masculino , Percas/microbiología , Siberia/epidemiologíaRESUMEN
The inhibitors produced by the parasitic worms successfully protect them from the host's proteases and are supposed to underlie the host-parasite specificity. Our previous study has shown that the extracts from the pike tapeworm Triaenophorus nodulosus inhibit host proteinases and commercial trypsin. We aimed to isolate and identify the components responsible for trypsin inactivation. After a two-step separation the molecular masses were measured by SE-HPLC. The sample proved to contain four fractions represented by polypeptides (1-45â¯kDa) and low-molecular hydrophobic compounds. According to SDS-PAGE analysis, the major polypeptides in the fractions displaying the highest inhibition had masses of 14.4â¯kDa. The study culminated in partial N-terminal amino acid sequence analysis with a further search for homology. The research revealed two novel Kunitz-type proteins potentially responsible for the inhibitory capacity of the tapeworms against trypsin. Our findings extend the list of cestodes relying on Kunitz-type proteins in the host-parasite molecular cross-talk.
Asunto(s)
Cestodos/metabolismo , Interacciones Huésped-Parásitos/fisiología , Inhibidores de Tripsina/química , Animales , Infecciones por Cestodos/metabolismo , Esocidae/parasitología , Tripsina/metabolismo , Inhibidores de Tripsina/aislamiento & purificaciónRESUMEN
Adaptive mechanisms underlying the long-term existence of intestinal parasites in their enzymatically hostile environment are still poorly understood, particularly with regard to fish cestodes. The study describes the activity distribution of proteolytic enzymes along the gut of the bream Abramis brama infected with intestinal cestodes Caryophyllaeus laticeps and characterizes the capacity of these worms to inhibit host proteinase activity. Mucosal proteolytic activity was mainly presented by serine proteinases. The research revealed an insignificant increase in total proteolytic activity from anterior and middle to posterior part of the gut accompanied with changes in proportions of various proteinase subclasses along the intestine. The trypsin (but not chymotrypsin) activity in the posterior section was significantly higher than in the mid-section. Both the incubation medium of the worms and their extract had a significant inhibitory effect on mucosal proteolytic activity and commercial trypsin samples. In both instances, the effect was comparable with that of a synthetic serine protease inhibitor, PMSF. SDS-PAGE electrophoregrams of the incubation medium of C. laticeps and its extract revealed three common protein bands, with apparent molecular masses from 19 to 47â¯kDa, possibly responsible for the worms' inhibitory capacities. According to casein-zymography performed, the target host proteinases for a putative cestode inhibitor (inhibitors) have an approximate molecular weight of 28-53â¯kDa. A comparative test with the extracts from three other cestodes showed that each of them can suppress the proteolytic activity of the bream mucosa. The level of inhibitory activity was found to increase with protein content in the extracts of these tapeworms.
Asunto(s)
Infecciones por Cestodos/veterinaria , Cyprinidae/metabolismo , Cyprinidae/parasitología , Enfermedades de los Peces/enzimología , Enfermedades de los Peces/parasitología , Péptido Hidrolasas/metabolismo , Animales , Cestodos/metabolismo , Cestodos/patogenicidad , Infecciones por Cestodos/enzimología , Infecciones por Cestodos/parasitología , Proteínas de Peces/aislamiento & purificación , Proteínas de Peces/metabolismo , Proteínas del Helminto/metabolismo , Interacciones Huésped-Parásitos , Mucosa Intestinal/enzimología , Mucosa Intestinal/parasitología , Peso Molecular , Péptido Hidrolasas/aislamiento & purificación , Perciformes , Inhibidores de Proteasas/metabolismoRESUMEN
Parasitic organisms inhabiting the alimentary canal should permanently resist the destructive action of host digestive enzymes. The intestinal parasites were shown to produce specific protease inhibitors protecting them from proteolysis. However, little is known about this adaptive mechanism in cestodes so far, especially for the tapeworms dwelling inside the fish intestines. Here, we explored the ability to inactivate proteolytic enzymes in the fish tapeworm Eubothrium rugosum (Batsch, 1786) (Bothriocephalidea) parasitising the intestine of wild burbot, Lota lota (Linnaeus). The assays were conducted with different concentrations of commercial trypsin and homogenate of intestinal mucosa both being the sources of proteinases. The incubation of live E. rugosum in trypsin solutions of two different concentrations caused a significant decrease in the enzyme activity. The extent of activity reduction was dependent on trypsin concentration. At the same time, the inhibitory effect of the worm incubation medium turned out to be statistically insignificant. These findings suggest partial adsorption of the enzyme to the tegument surface, with its further inactivation. In contrast to the incubation medium, the worm extract suppressed over 80% of trypsin activity and nearly half of the proteolytic activity in the mucosa homogenate. Notably, the inhibitory activity of the tapeworms hardly depended on their size characteristics. Finally, the research has demonstrated secretion of proteinase inhibitor in E. rugosum, which appears to be essential for its survival in enzymatically hostile environment.
Asunto(s)
Cestodos/enzimología , Infecciones por Cestodos/veterinaria , Enfermedades de los Peces/parasitología , Péptido Hidrolasas/metabolismo , Animales , Cestodos/aislamiento & purificación , Infecciones por Cestodos/parasitología , Gadiformes , Inhibidores de Proteasas , Tripsina/metabolismoRESUMEN
The activities of major digestive hydrolases (proteases, amylase, lipase and esterases) along the intestine were studied in the burbot Lota lota (L.) using different methods of activity expression. The enzyme activities were determined both in the whole gut segments and in the isolated mucosa, and then expressed in terms of tissue mass and protein content in the samples. Further, the cumulative activities of these enzymes in the pyloric caeca were compared with those in the rest of the intestine to estimate the overall contribution of these regions to digestion. The data obtained suggest the essential role of the pyloric caeca in the digestion of the burbot. In addition, the variations in the pH values along the intestine and the changes in the enzyme activities with incubation temperature were examined. The study proved the method of enzyme activity expression to be a key factor influencing the outcome of the experiment.