RESUMEN

Changes in the refractive index of the cytoplasm and the affinity of haemoporphyrin of erythrocyte haemoglobin to oxygen (pH, 2,3-diphosphoglycerate) have been investigated using laser interference microscopy and Raman spectroscopy. It has been established that a decrease in pH and an increase in the content of 2,3-diphosphoglycerate are accompanied by changes in both the form of the cell and the refractive index of the cytoplasm and the affinity of haemoporphyrin of hemoglobin to oxygen. It has been shown that as pH is reduced, the capacity of haemoporphyrin for binding oxygen decreases and as the concentration of 2,3-diphosphoglycerate is increased, the ability of haemoporphyrin for oxygen reabsorption increases.

Asunto(s)

Citoplasma/ultraestructura , Eritrocitos/metabolismo , Hemoglobinas/metabolismo , Oxígeno/metabolismo , 2,3-Difosfoglicerato/farmacología , Forma de la Célula , Eritrocitos/citología , Eritrocitos/efectos de los fármacos , Líquido Extracelular/química , Hematoporfirinas/química , Hematoporfirinas/metabolismo , Hemoglobinas/química , Humanos , Concentración de Iones de Hidrógeno , Microscopía Confocal , Microscopía de Interferencia , Conformación Proteica , Espectrometría Raman