RESUMEN
Genome engineering of T lymphocytes, the main effectors of antitumor adaptive immune responses, has the potential to uncover unique insights into their functions and enable the development of next-generation adoptive T cell therapies. Viral gene delivery into T cells, which is currently used to generate CAR T cells, has limitations in regard to targeting precision, cargo flexibility, and reagent production. Nonviral methods for effective CRISPR/Cas9-mediated gene knock-out in primary human T cells have been developed, but complementary techniques for nonviral gene knock-in can be cumbersome and inefficient. Here, we report a convenient and scalable nonviral method that allows precise gene edits and transgene integration in primary human T cells, using plasmid donor DNA template and Cas9-RNP. This method is highly efficient for single and multiplex gene manipulation, without compromising T cell function, and is thus valuable for use in basic and translational research.
Asunto(s)
Sistemas CRISPR-Cas , Edición Génica , Sistemas CRISPR-Cas/genética , ADN/genética , Edición Génica/métodos , Humanos , Plásmidos/genética , Linfocitos TRESUMEN
In the original PDF version of this Article, which was published on 16 October 2017, the publication date was incorrectly given as 11 October 2017. This has now been corrected in the PDF; the HTML version of the paper was correct from the time of publication.
RESUMEN
Zbtb16-encoded PLZF is a signature transcription factor (TF) that directs the acquisition of T-helper effector programs during the development of multiple innate lymphocyte lineages, including natural killer T cell, innate lymphoid cell, mucosal-associated invariant T cell and γδ lineages. PLZF is also essential in osteoblast and spermatogonial development. How Zbtb16 itself is regulated in different lineages is incompletely understood. Here, by systematic CRISPR/Cas9-assisted deletions of chromatin accessible regions within the Zbtb16 locus in mouse, we identify a critical enhancer controlling PLZF expression exclusively in innate lymphoid lineages. Multiple sites within this enhancer express canonical motifs for the TF Runx1, which is essential for the development of these lineages. Notably, some regulatory sites control the kinetic rather than the overall level of PLZF expression. Thus, our comprehensive, unbiased analysis of regulatory elements in vivo reveals critical mechanisms of Zbtb16 regulation shared between innate and innate-like lymphoid lineages. Zbtb16-encoded transcription factor PLZF directs the differentiation of multiple innate and innate-like cell lineages, but how Zbtb16 itself is regulated remains unclear. Here the authors show, using CRISPR gene editing, ATAC-seq and ChIP-seq, that specific Runx1-bound enhancer elements critically modulate lineage-dependent expressions of PLZF.
Asunto(s)
Subunidad alfa 2 del Factor de Unión al Sitio Principal/genética , Linfocitos Intraepiteliales/inmunología , Linfocitos/inmunología , Células T Asesinas Naturales/inmunología , Proteína de la Leucemia Promielocítica con Dedos de Zinc/genética , Linfocitos T Colaboradores-Inductores/inmunología , Animales , Sistemas CRISPR-Cas , Diferenciación Celular , Linaje de la Célula , Elementos de Facilitación Genéticos , Epigénesis Genética , Inmunidad Innata/inmunología , Linfocitos Intraepiteliales/citología , Linfocitos/citología , Linfopoyesis , Ratones , Ratones Noqueados , Células T Asesinas Naturales/citología , Proteína de la Leucemia Promielocítica con Dedos de Zinc/inmunología , Secuencias Reguladoras de Ácidos Nucleicos , Linfocitos T Colaboradores-Inductores/citologíaRESUMEN
The discovery of tissue-resident innate lymphoid cell populations effecting different forms of type 1, 2, and 3 immunity; tissue repair; and immune regulation has transformed our understanding of mucosal immunity and allergy. The emerging complexity of these populations along with compounding issues of redundancy and plasticity raise intriguing questions about their precise lineage relationship. Here we review advances in mapping the emergence of these lineages from early lymphoid precursors. We discuss the identification of a common innate lymphoid cell precursor characterized by transient expression of the transcription factor PLZF, and the lineage relationships of innate lymphoid cells with conventional natural killer cells and lymphoid tissue inducer cells. We also review the rapidly growing understanding of the network of transcription factors that direct the development of these lineages.
Asunto(s)
Diferenciación Celular , Hipersensibilidad/inmunología , Inmunidad Innata , Linfocitos/inmunología , Células Progenitoras Linfoides/inmunología , Animales , Linaje de la Célula , Citocinas/metabolismo , Regulación de la Expresión Génica/inmunología , Redes Reguladoras de Genes , Humanos , Factores de Transcripción de Tipo Kruppel/genética , Factores de Transcripción de Tipo Kruppel/metabolismo , Proteína de la Leucemia Promielocítica con Dedos de Zinc , Células TH1/inmunología , Células Th2/inmunologíaRESUMEN
The precise lineage relationship between innate lymphoid cells (ILCs) and lymphoid tissue-inducer (LTi) cells is poorly understood. Using single-cell multiplex transcriptional analysis of 100 lymphoid genes and single-cell cultures of fetal liver precursor cells, we identified the common proximal precursor to these lineages and found that its bifurcation was marked by differential induction of the transcription factors PLZF and TCF1. Acquisition of individual effector programs specific to the ILC subsets ILC1, ILC2 and ILC3 was initiated later, at the common ILC precursor stage, by transient expression of mixed ILC1, ILC2 and ILC3 transcriptional patterns, whereas, in contrast, the development of LTi cells did not go through multilineage priming. Our findings provide insight into the divergent mechanisms of the differentiation of the ILC lineage and LTi cell lineage and establish a high-resolution 'blueprint' of their development.
Asunto(s)
Linaje de la Célula/inmunología , Subgrupos Linfocitarios/inmunología , Linfocitos/inmunología , Linfocitos T Colaboradores-Inductores/inmunología , Animales , Diferenciación Celular/genética , Citometría de Flujo , Perfilación de la Expresión Génica , Factor Nuclear 1-alfa del Hepatocito/inmunología , Factores de Transcripción de Tipo Kruppel/genética , Factores de Transcripción de Tipo Kruppel/inmunología , Tejido Linfoide/citología , Ratones , Reacción en Cadena de la Polimerasa Multiplex , Proteína de la Leucemia Promielocítica con Dedos de Zinc , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Análisis de la Célula IndividualRESUMEN
Immunoglobulin A (IgA) is prominently secreted at mucosal surfaces and coats a fraction of the intestinal microbiota. However, the commensal bacteria bound by IgA are poorly characterized and the type of humoral immunity they elicit remains elusive. We used bacterial flow cytometry coupled with 16S rRNA gene sequencing (IgA-Seq) in murine models of immunodeficiency to identify IgA-bound bacteria and elucidate mechanisms of commensal IgA targeting. We found that residence in the small intestine, rather than bacterial identity, dictated induction of specific IgA. Most commensals elicited strong T-independent (TI) responses that originated from the orphan B1b lineage and from B2 cells, but excluded natural antibacterial B1a specificities. Atypical commensals including segmented filamentous bacteria and Mucispirillum evaded TI responses but elicited T-dependent IgA. These data demonstrate exquisite targeting of distinct commensal bacteria by multiple layers of humoral immunity and reveal a specialized function of the B1b lineage in TI mucosal IgA responses.
Asunto(s)
Inmunidad Adaptativa/inmunología , Bacterias/inmunología , Inmunidad Humoral/inmunología , Inmunidad Innata/inmunología , Inmunoglobulina A/inmunología , Intestino Delgado/inmunología , Inmunidad Adaptativa/genética , Animales , Linfocitos B/inmunología , Linfocitos B/metabolismo , Bacterias/clasificación , Bacterias/genética , Colon/inmunología , Colon/metabolismo , Colon/microbiología , Citometría de Flujo , Variación Genética/inmunología , Humanos , Inmunidad Humoral/genética , Inmunidad Innata/genética , Inmunoglobulina A/metabolismo , Intestino Delgado/metabolismo , Intestino Delgado/microbiología , Ratones Endogámicos C57BL , Ratones Noqueados , ARN Ribosómico 16S/genética , Linfocitos T/inmunología , Linfocitos T/metabolismoRESUMEN
Among the variety of tissue-resident NK-like populations recently distinguished from recirculating classical NK (cNK) cells, liver innate lymphoid cells (ILC) type 1 (ILC1s) have been shown to represent a distinct lineage that originates from a novel promyelocytic leukaemia zinc finger (PLZF)-expressing ILC precursor (ILCP) strictly committed to the ILC1, ILC2, and ILC3 lineages. Here, using PLZF-reporter mice and cell transfer assays, we studied the developmental progression of ILC1s and demonstrated substantial overlap with stages previously ascribed to the cNK lineage, including pre-pro-NK, pre-NK precursor (pre-NKP), refined NKP (rNKP), and immature NK (iNK). Although they originated from different precursors, the ILC1 and cNK lineages followed a parallel progression at early stages and diverged later at the iNK stage, with a striking predominance of ILC1s over cNKs early in ontogeny. Although a limited set of ILC1 genes depended on PLZF for expression, characteristically including Il7r, most of these genes were also differentially expressed between ILC1s and cNKs, indicating that PLZF together with other, yet to be defined, factors contribute to the divergence between these lineages.
Asunto(s)
Linaje de la Célula , Inmunidad Innata , Factores de Transcripción de Tipo Kruppel/metabolismo , Linfocitos/citología , Linfocitos/metabolismo , Animales , Animales Recién Nacidos , Antígenos Ly/metabolismo , Células de la Médula Ósea/citología , Diferenciación Celular/genética , Linaje de la Célula/genética , Feto/citología , Perfilación de la Expresión Génica , Inmunidad Innata/genética , Células Asesinas Naturales/citología , Células Asesinas Naturales/inmunología , Células Asesinas Naturales/metabolismo , Hígado/citología , Hígado/embriología , Ratones Endogámicos C57BL , Subfamilia B de Receptores Similares a Lectina de Células NK/metabolismo , Proteína de la Leucemia Promielocítica con Dedos de Zinc , Células Madre/citología , Células Madre/inmunologíaRESUMEN
The origin and developmental pathway of intestinal T cell receptor αß(+) CD4(-)CD8ß(-) intraepithelial lymphocytes (unconventional iIELs), a major population of innate-like resident cytolytic T cells, have remained elusive. By cloning and expressing several TCRs isolated from unconventional iIELs, we identified immature CD4(lo)CD8(lo)(DP(lo))CD69(hi)PD-1(hi) thymocytes as the earliest postsignaling precursors for these cells. Although these precursors displayed multiple signs of elevated TCR signaling, a sizeable fraction of them escaped deletion to selectively engage in unconventional iIEL differentiation. Conversely, TCRs cloned from DP(lo)CD69(hi)PD-1(hi) thymocytes, a population enriched in autoreactive thymocytes, selectively gave rise to unconventional iIELs upon transgenic expression. Thus, the unconventional iIEL precursor overlaps with the DP(lo) population undergoing negative selection, indicating that, concomitant with the downregulation of both CD4 and CD8 coreceptors, a balance between apoptosis and survival signals results in outcomes as divergent as clonal deletion and differentiation to the unconventional iIEL lineage.