RESUMEN
BACKGROUND: Some studies have listed motions that may cause Osgood-Schlatter disease, but none have quantitatively assessed the load on the tibial tubercle by such motions. PURPOSES: To quantitatively identify the load on the tibial tubercle through a biomechanical approach using various motions that may cause Osgood-Schlatter disease, and to compare the load between different motions. METHODS: Eight healthy male subjects were included. They conducted 4 types of kicks with a soccer ball, 2 types of runs, 2 types of squats, 2 types of jump landings, 2 types of stops, 1 type of turn, and 1 type of cutting motion. The angular impulse was calculated for knee extension moments ≥1.0 Nm/kg, ≥1.5 Nm/kg, ≥2.0 Nm/kg, and ≥2.5 Nm/kg. After analysis of variance, the post-hoc test was used to perform pairwise comparisons between all groups. RESULTS/CONCLUSIONS: The motion with the highest mean angular impulse of knee extension moment ≥1.0 Nm/kg was the single-leg landing after a jump, and that with the second highest mean was the cutting motion. At ≥1.5 Nm/kg, ≥2.0 Nm/kg, and ≥2.5 Nm/kg, the cutting motion was the highest, followed by the jump with a single-leg landing. They have a large load, and are associated with a higher risk of developing Osgood-Schlatter disease. The mean angular impulse of the 2 types of runs was small at all the indicators. CLINICAL RELEVANCE: Motions with a high risk of developing Osgood-Schlatter disease and low-risk motions can be assessed in further detail if future studies can quantify the load and number of repetitions that may cause Osgood-Schlatter disease while considering age and the development stage. Scheduled training regimens that balance load on the tibial tubercle with low-load motions after a training day of many load-intensive motions may prevent athletes from developing Osgood-Schlatter disease and increase their participation in sports.
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Movimiento (Física) , Osteocondrosis/fisiopatología , Deportes , Adulto , Humanos , Articulación de la Rodilla/fisiopatología , Masculino , Medición de Riesgo , Adulto JovenRESUMEN
When the medical fee system was revised in 2012, the category of perioperative oral management was newly organized. However, the calculation of additional fees for such management required referral from medical to dental departments. In addition, requests for such management were limited, possibly owing to an increased burden on doctors engaged in outpatient services. This study examined the usefulness of an approach to promote patients' use of dental services by increasing their awareness of the importance of oral management. In this approach, pharmacists explained doctors' instructions to patients at a chemotherapy center within the study facility. Explanations were provided to 114 patients, 75 (65.8%) of whom subsequently used dental services in the facility. For patients using dental services, oral care was performed most frequently (40; 53.3%), followed by invasive procedures (23; 30.7%). Furthermore, the facility's ethics committee approved a survey to measure the satisfaction of patients undergoing chemotherapy at the center. Of the 110 patients invited to participate in the survey, 77(70.0%) did not respond. Researchers concluded the low response rate was associated with patients' belief that dental services were intended primarily for treating oral cavities and their lack of awareness of the importance of preventive dental care. However, in 2014, the number of calculations of additional fees for perioperative oral management markedly increased each month after the above-mentioned approach, from 62 (January) to 162 (December). Both the hospital-to family and family-to-hospital dentist referral rates significantly increased, from 11.2% and 10.7%, respectively (June 2013), to 21.0% and 41.9%, respectively(June 2014). Future evaluations of the outcomes of perioperative oral management and promoting cooperation between medical and dental communities may be necessary.
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Higiene Bucal , Grupo de Atención al Paciente , Encuestas y Cuestionarios , Adulto , Anciano , Anciano de 80 o más Años , Humanos , Persona de Mediana Edad , Satisfacción del Paciente , Rol Profesional , Adulto JovenRESUMEN
New soccer shoes have been developed by considering various concepts related to kicking, such as curving a soccer ball. However, the effects of shoes on ball behaviour remain unclear. In this study, by using a finite element simulation, we investigated the factors that affect ball behaviour immediately after impact in a curve kick. Five experienced male university soccer players performed one curve kick. We developed a finite element model of the foot and ball and evaluated the validity of the model by comparing the finite element results for the ball behaviour immediately after impact with the experimental results. The launch angle, ball velocity, and ball rotation in the finite element analysis were all in general agreement with the experimental results. Using the validated finite element model, we simulated the ball behaviour. The simulation results indicated that the larger the foot velocity immediately before impact, the larger the ball velocity and ball rotation. Furthermore, the Young's modulus of the shoe upper and the coefficient of friction between the shoe upper and the ball had little effect on the launch angle, ball velocity, and ball rotation. The results of this study suggest that the shoe upper does not significantly influence ball behaviour.
RESUMEN
Nasal natural killer (NK)/T-cell lymphoma (NNKTL) is an Epstein-Barr virus (EBV)-related malignancy with poor prognosis and has distinct histological features characterized by angiocentric and polymorphous lymphoreticular infiltrates including inflammatory cells such as granulocytes, monocytes, macrophages and lymphocytes. Here, we show that the monocytes enhance proliferation as well as LMP1 expression of NNKTL cells by cell contact-dependent interaction through membrane-bound interleukin (IL)-15. We used two EBV-positive NK-cell lines, SNK6 and KAI3, which originated from two patients-SNK6 from a patient with NNKTL and KAI3 from a patient with a severe mosquito allergy. We cocultured the cell lines with granulocytes or monocytes and examined whether proliferation, survival and LMP1 expression of the cells changed. Although cocultured granulocytes did not affect proliferation, survival or LMP1 expression of the cells, cocultured monocytes enhanced both proliferation and LMP1 expression in a dose-dependent manner. These phenomena were not seen when monocytes were placed in a separate chamber. Moreover, the monocyte-inducible proliferation and LMP1 expression were inhibited by treatment with an antibody against IL-15. Furthermore, production of interferon-gamma-inducible protein (IP)-10 were enhanced by coculture with monocytes and were inhibited by the antibody. Immunohistological studies confirmed that a number of infiltrating CD14-positive monocytes contacted CD56-positive lymphoma cells in all of 20 NNKTL tissues tested. These results suggest that monocytes enhance cell growth as well as LMP1 expression of NNKTL cells by cell contact-dependent interaction through membrane-bound IL-15. In the microenvironment of NNKTL tissue, a positive feedback loop of interaction between lymphoma cells and monocytes may be present and contribute to lymphoma progression.
Asunto(s)
Proliferación Celular , Interleucina-15/metabolismo , Células Asesinas Naturales/patología , Linfoma de Células T/patología , Monocitos/patología , Neoplasias Nasales/patología , Proteínas de la Matriz Viral/metabolismo , Western Blotting , Comunicación Celular , Membrana Celular/metabolismo , Membrana Celular/patología , Técnicas de Cocultivo , Ensayo de Inmunoadsorción Enzimática , Infecciones por Virus de Epstein-Barr/metabolismo , Infecciones por Virus de Epstein-Barr/patología , Infecciones por Virus de Epstein-Barr/virología , Citometría de Flujo , Granulocitos/metabolismo , Granulocitos/patología , Herpesvirus Humano 4 , Humanos , Técnicas para Inmunoenzimas , Interferón gamma , Células Asesinas Naturales/metabolismo , Células Asesinas Naturales/virología , Linfoma de Células T/metabolismo , Linfoma de Células T/virología , Monocitos/metabolismo , Monocitos/virología , Neoplasias Nasales/metabolismo , Neoplasias Nasales/virología , Células Tumorales CultivadasRESUMEN
The objective of this study was to investigate the factors affecting ball velocity at the final instant of the impact phase (t1) in full instep soccer kicking. Five experienced male university soccer players performed maximal full instep kicks for various foot impact points using a one-step approach. The kicking motions were captured two dimensionally by a high-speed camera at 2,500 fps. The theoretical equation of the ball velocity at t1 given in the article was derived based on the impact dynamics theory. The validity of the theoretical equation was verified by comparing the theoretical relationship between the impact point and the ball velocity with the experimental one. Using this theoretical equation, the relationship between the impact point and the ball velocity was simulated. The simulation results indicated that the ball velocity is more strongly affected by the foot velocity at the initial instant of the impact phase than by other factors. The simulation results also indicated that decreasing the ankle joint reaction force during ball impact shifts the impact point that produces the greatest ball velocity to the toe side and decreasing the ankle joint torque during ball impact shifts the impact point that produces the greatest ball velocity to the ankle side.
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Tobillo/fisiopatología , Pie/fisiología , Modelos Biológicos , Movimiento/fisiología , Fútbol/fisiología , Equipo Deportivo , Simulación por Computador , Humanos , Masculino , Torque , Adulto JovenRESUMEN
The differences between the assessments performed with and without the point cluster technique (PCT) for knee joint motions during the high-risk movements associated with non-contact anterior cruciate ligament (ACL) injuries have not been reported. This study aims to examine the differences between PCT and non-PCT assessments for knee joint angles and moments during shuttle run cutting. Fourteen high school athletes performed a maximal effort shuttle run cutting task. Motion data were collected by an 8-camera motion analysis system at 200 Hz, and ground reaction force data were recorded using a force plate at 1000 Hz. In both PCT and non-PCT approaches, the knee joint angles were calculated using Euler angle rotations, and the knee joint moments were obtained by solving the Newton-Euler equations using an inverse dynamics technique. For the extension/flexion angle, good agreement was measured between PCT and non-PCT assessments. The abduction angle obtained in the non-PCT assessment was smaller than that obtained with the PCT. An internal rotation angle was obtained in the PCT assessment, whereas a small external rotation angle was obtained in the non-PCT assessment. For the knee joint moments, good agreement between PCT and non-PCT assessments was observed for all the components. The differences in the knee joint angles were attributed in part to the differences in the position of the medial femoral epicondyle. The results suggest that the ACL injury risk during shuttle run cutting is estimated lower in the non-PCT assessment than in the PCT assessment.
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Lesiones del Ligamento Cruzado Anterior , Articulación de la Rodilla/fisiología , Fenómenos Biomecánicos , Fémur/patología , Humanos , Inestabilidad de la Articulación , Cinética , Traumatismos de la Rodilla , Articulación de la Rodilla/patología , Modelos Anatómicos , Movimiento (Física) , Movimiento , Reproducibilidad de los Resultados , Factores de Riesgo , Carrera , Tibia/patologíaRESUMEN
PURPOSE: Nasal natural killer (NK)/T-cell lymphoma is associated with Epstein-Barr virus and has poor prognosis because of local invasion and/or multiple dissemination. Recently, the role of chemokines/chemokine receptors in tumor proliferation and invasion has been shown. In this study, we examined whether the specific chemokines were related to the tumor behaviors in nasal NK/T-cell lymphoma. EXPERIMENTAL DESIGN: A chemokine protein array was used to examine specific chemokines produced by SNK-6 and SNT-8 (Epstein-Barr virus-positive nasal NK/T-cell lymphoma lines). The expression of interferon gamma-inducible protein 10 (IP-10) and the IP-10 receptor CXCR3 was investigated by ELISA and flow cytometry. Cell growth and invasion were assessed by the MTT and Matrigel invasion assays, respectively. Immunohistologic staining and ELISA were used to examine IP-10 expression in biopsies and sera from patients, respectively. RESULTS: IP-10 was specifically produced by SNK-6 and SNT-8. Moreover, CXCR3 was expressed on the NK cell lines. Functionally, IP-10 did not affect cell proliferation but enhanced cell invasion. In biopsy samples, IP-10 and CXCR3 expressions were detected in the lymphoma cells. Serum IP-10 levels in the patients were much higher than those of healthy controls and the levels were decreased during the complete remission phase after treatments. CONCLUSIONS: These results suggest that IP-10 may play an important role in cell invasion in nasal NK/T-cell lymphoma through an autocrine mechanism.
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Quimiocina CXCL10/biosíntesis , Células Asesinas Naturales/metabolismo , Linfoma de Células T/metabolismo , Adulto , Anciano , Biopsia , Línea Celular Tumoral , Proliferación Celular , Quimiocinas/metabolismo , Femenino , Humanos , Masculino , Persona de Mediana Edad , Invasividad Neoplásica , Inducción de RemisiónRESUMEN
This study examined the factors affecting the ball velocity and rotation for side-foot soccer kick using a numerical investigation. Five experienced male university soccer players performed side-foot kicks with various attack angles and impact points using a one-step approach. The kicking motions were captured three-dimensionally by two high-speed cameras at 2500 fps. The theoretical equations of the ball velocity and rotation were derived based on impact dynamic theory. Using the theoretical equations, the relationships of the ball velocity and rotation to the attack angle and impact point were obtained. The validity of the theoretical equations was verified by comparing the theoretical relationships with measurement values. Furthermore, simulations of the ball velocity and rotation were conducted using the theoretical equations. The theoretical relationships were in good agreement with the measurement values. The theoretical results confirmed the previously reported experimental results, and indicated that the impact point is more influential on the ball velocity than the attack angle and the attack angle is more influential on the ball rotation than the impact point. The simulation results indicated the following. The ball velocity produced by impact for all impact patterns is largely affected by the foot velocity immediately before impact but barely affected by the degree of slip between the foot and the ball. The ball rotation produced by an impact with a large attack angle is affected by the foot velocity immediately before impact and the degree of slip between the foot and the ball; however, these factors affect the ball rotation less than the attack angle.
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Pierna/fisiología , Modelos Biológicos , Movimiento/fisiología , Fútbol/fisiología , Equipo Deportivo , Análisis y Desempeño de Tareas , Simulación por Computador , Humanos , Masculino , Movimiento (Física) , Estrés Mecánico , Adulto JovenRESUMEN
Nasal natural killer (NK)/T-cell lymphoma (NNKTL) is a clinical illness characterized by progressive unrelenting ulceration and necrosis of the nasal cavity and midline facial tissues. Histological features of the lymphoma include angiocentric and polymorphous lymphoreticular infiltrates, called polymorphic reticulosis. Surface antigens and the NK-cell marker, CD56, as well as pan-T antigen CD2, cytoplasmic CD3 (CD3epsilon), and CD45 are expressed in the lymphoma cells. The origin of the lymphoma is thought to be either NK-cell linkage without T-cell receptor (TCR) rearrangement or gammadeltaT-cell linkage with gammadeltaTCR rearrangement. Since the authors of this study first demonstrated the presence of Epstein Barr virus (EBV)-DNA and EBV oncogenic proteins in NNKTL, the lymphoma has been classified as one of the EBV-associated malignancies. The NNKTL cells produce interleukin (IL)-9, IL-10, and interferon-gamma-inducible protein-10 (IP-10), possibly due to EBV-oncogenic proteins in the lymphoma cells, and such cytokines take an important part in the cell proliferation and invasion, acting in an autocrine manner. Clinically, the serum EBV-DNA copy number is useful as a specific tumor marker and a predictive prognostic factor. Even in early clinical stages, the lymphoma shows poor prognosis caused by the rapid progression of the lesion into distinct organs. Our newly designed arterial infusion chemotherapy, from the superficial temporal artery, in combination with radiotherapy, has shown a favorable outcome in patients with NNKTL. In this article, the clinical, pathological, and virological characteristics of the lymphoma are reviewed, along with a report of our investigations.
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Herpesvirus Humano 4/aislamiento & purificación , Células Asesinas Naturales/patología , Linfoma de Células T , Neoplasias Nasales , Quimiocinas/biosíntesis , Citocinas/biosíntesis , Humanos , Linfoma de Células T/diagnóstico , Linfoma de Células T/genética , Linfoma de Células T/inmunología , Linfoma de Células T/terapia , Mutación , Neoplasias Nasales/diagnóstico , Neoplasias Nasales/genética , Neoplasias Nasales/inmunología , Neoplasias Nasales/terapia , PronósticoRESUMEN
Nasal natural killer (NK)/T cell lymphoma is a peculiar lymphoma with a unique immunophenotype. Etiologically, in 1990, the authors first demonstrated the presence of Epstein-Barr virus (EBV) genomes and their products in this lymphoma. EBV-specific cytotoxic T lymphocytes (CTL) are very important in controlling the long-term persistence of EBV infection. Amino acid changes encoding the CTL epitope on the lymphoma cells may result in a reduced CTL response. We focused on two major CTL epitopes SSCSSCPLSK (codon 340 to 349) and FLYALALLLL (codon 356 to 364) of the LMP2A gene and determined the sequence isolated from nasal NK/T cell lymphoma tissues. All isolates from 7 nasal NK/T cell lymphomas showed the same amino acid change from serine to threonine at codon 348 in the CTL epitope SSCSSCPLSK. Threonine or serine substitution at codon 348 was almost equally observed in peripheral blood EBV isolates from healthy individuals in various ethnic origins. The predominant threonine substitution of nasal NK/T cell lymphoma patients may represent disease-associated polymorphism rather than a geographic or race-associated polymorphism. The LMP2A strain including threonine substitution at codon 348 may be selected within tumors and play a role for tumor genesis in Japanese patients with nasal NK/T cell lymphoma through reduced immune recognition.
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Sustitución de Aminoácidos/genética , Antígenos Virales/genética , Epítopos de Linfocito T/genética , Herpesvirus Humano 4/genética , Linfoma de Células T/virología , Neoplasias Nasales/virología , Proteínas de la Matriz Viral/genética , Adulto , Anciano , Pueblo Asiatico , Femenino , Herpesvirus Humano 4/aislamiento & purificación , Humanos , Masculino , Persona de Mediana Edad , Mutación Missense , Análisis de Secuencia de ADNRESUMEN
Quantitative real-time polymerase chain reaction (PCR) was utilized to measure serum EBV DNA levels of BamHI W fragment and latent membrane protein 1 (LMP1) in 20 nasal natural killer (NK)/T-cell lymphoma patients. Both serum EBV DNAs were detected at high levels in all patients, but the levels were below the limit of detection in all healthy controls. The BamHI Z fragment, Epstein-Barr-replication activator (ZEBRA) expression was detected in a small proportion (0.1-3%) of lymphoma cells from 10 (50%) of the patients. Patients with ZEBRA expression showed significantly higher DNA levels of BamHI W and LMP1 (P = 0.0081, P = 0.004), suggesting that EBV DNA may be caused by EBV replication from lymphoma cells. Kaplan-Meier and univariate analyses revealed that high DNA levels of BamHI W and LMP1 at pre-treatment and high BamHI W DNA level at post-treatment were associated with short disease-free survival and overall survival (P < 0.05, each). Although the DNA levels of BamHI W and LMP1 correlated significantly, their dynamics were not always parallel. Patients with low pre-treatment level of both EBV DNAs showed a favorable course, in contrast to patients with high pre-treatment level of both EBV DNAs who showed an aggressive course (P = 0.0085). More importantly, the high pre-treatment level of both EBV DNAs was determined as the only independent prognostic factor among various prognostic factors. These data suggest that simultaneous measurement of serum levels of both BamHI W and LMP1 DNAs may be useful for diagnosis, disease monitoring, and prediction of prognosis for nasal NK/T-cell lymphoma patients.
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ADN Viral/sangre , Desoxirribonucleasa BamHI/genética , Herpesvirus Humano 4/aislamiento & purificación , Células Asesinas Naturales , Linfoma de Células T/diagnóstico , Neoplasias Nasales/diagnóstico , Proteínas de la Matriz Viral/genética , Proteínas Virales/genética , Adulto , Anciano , Antígenos Virales , Biomarcadores/sangre , Cartilla de ADN , Femenino , Herpesvirus Humano 4/genética , Humanos , Linfoma de Células T/sangre , Masculino , Persona de Mediana Edad , Neoplasias Nasales/sangre , Fragmentos de Péptidos/genética , Reacción en Cadena de la Polimerasa/métodos , PronósticoRESUMEN
We have investigated the association of immunoescape mechanisms in nasopharyngeal carcinoma (NPC) lesions with Epstein-Barr virus (EBV) infection and clinical course of the disease. Tumor biopsy specimens obtained from 36 Japanese NPC patients were examined for antigen processing machinery component and HLA class I antigen expression, CD8(+) T cell infiltration, and Fas, Fas ligand (FasL) and IL-10 expression using immunohistochemical staining. The results were correlated with the histopathological characteristics of the lesions, the clinical course of the disease and EBV infection. LMP2, TAP1, tapasin and HLA class I antigens were downregulated in more than 65% of the lesions tested, while FasL, Fas and IL-10 were expressed in at least 60% of the lesions. Statistical analysis showed that (i) HLA class I antigen expression was significantly correlated with LMP2 and tapasin expression (r = 0.39 and 0.45, respectively); (ii) CD8(+) T cell infiltration into tumor lesions was significantly correlated with HLA class I antigen, LMP2 and Fas expression (r = 0.34, 0.49 and 0.44, respectively); (iii) LMP2 and FasL expression was significantly correlated with IL-10 expression (r = 0.49 and 0.52, respectively); (iv) IL-10 expression was significantly associated with EBERs and EBV oncoprotein LMP1 expression (p = 0.00078 and 0.015, respectively) and (v) FasL overexpression was significantly associated with reduced patients' survival (p = 0.033). Multivariate analysis identified FasL overexpression as an independent unfavorable prognostic marker. These results suggest that NPC cells may utilize multiple immunoescape mechanisms, including dysfunction of HLA class I antigens and Fas/FasL apoptosis pathways. Furthermore, FasL expression appears to be associated with IL-10 upregulation in EBV positive NPC cells.
Asunto(s)
Herpesvirus Humano 4/aislamiento & purificación , Neoplasias Nasofaríngeas/inmunología , Neoplasias Nasofaríngeas/virología , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Linfocitos T CD8-positivos/inmunología , Proteína Ligando Fas/inmunología , Femenino , Antígenos de Histocompatibilidad Clase I/inmunología , Humanos , Inmunohistoquímica , Interleucina-10/inmunología , Masculino , Persona de Mediana Edad , ARN Mensajero/genética , Receptor fas/inmunologíaRESUMEN
Nasal natural killer (NK)/T-cell lymphoma is a peculiar lymphoma with an unique immunophenotype. Etiologically, the authors previously first demonstrated the presence of Epstein-Barr virus (EBV) genomes and their products in this lymphoma (Lancet 1990; 335). It is suggested that some of sequence variations such as a 30-bp deletion and multiple base substitutions and as amino acid changes at HLA-A2 restricted CTL epitopes were associated with an increase in tumorigenicity and with a decrease in immune recognition. In this study, we determined full-length of LMP1 sequence isolated from 7 patients with nasal NK/T-cell lymphoma using polymerase chain reaction (PCR) method and compared the sequences with those referred to previous reports. In the carboxyl-terminal site, all 7 patients showed 4 copies of the 11 amino acids repeat (codon 254-302) and 30-bp deletion corresponding to codon 343-352 of the B95-8 strain. Within the NF-kB-activating domains, all 7 patients showed amino acid changes at codon 189 (Gln to Pro), 192 (Ser to Thr) and 212 (Gly to Ser) on either site of the PXQXT (codon 204-208) motif. In the major HLA-A2 restricted T-cell epitope sequence YLLEMLWRL (codon 125-133), all 7 patients showed amino acid changes at codon 126 (Leu to Phe) and 129 (Met to Ile). In the epitopes ALLVLYSFA (codon 51-59), VLFIFGCLL (codon 110-118) and WLLLFLAIL (codon 173-181), several patients showed novel amino acid changes at codon 59 (Ala to Gly), 110 (Val to Leu) and 174 (Leu to Ile), respectively. Although it is still not clear what the most specific and biologic variation of LMP1 gene in nasal NK/T-cell lymphoma is, the sequence data may be valuable on the study for pathogenesis of nasal NK/T-cell lymphoma and EBV molecular epidemiology.
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Variación Genética , Herpesvirus Humano 4/genética , Linfoma no Hodgkin/virología , Linfoma de Células T/virología , Mucosa Nasal/virología , Proteínas de la Matriz Viral/genética , Adulto , Anciano , Secuencia de Aminoácidos , Secuencia de Bases , Dominio Catalítico , Enzimas de Restricción del ADN/metabolismo , Femenino , Humanos , Células Asesinas Naturales/patología , Células Asesinas Naturales/virología , Masculino , Persona de Mediana Edad , Datos de Secuencia Molecular , Mucosa Nasal/patologíaRESUMEN
We have investigated the role of antigen-processing machinery (APM) component defects in HLA class I antigen down-regulation in laryngeal squamous cell carcinoma (SCC) lesions and assessed the clinical significance of these defects. To this end, 63 formalin-fixed, paraffin-embedded tumor lesions were examined for APM component and HLA class I antigen expression by immunohistochemistry. Calnexin, calreticulin, and ERp57 were down-regulated in approximately 25% of the lesions tested, whereas LMP2, TAP1, tapasin, and HLA class I antigens were down-regulated in at least 70% of the lesions tested. LMP2 and tapasin expression was significantly correlated with HLA class I antigen expression suggesting APM component defects as a mechanism underlying HLA class I antigen down-regulation in laryngeal SCC lesions. The expression of most APM components and HLA class I antigens was correlated with the extent of CD8+ T cell infiltration into tumor lesions. Furthermore, LMP2 and HLA class I antigen down-regulation and low CD8+ T cell infiltration were significantly associated with reduced patients' survival. Multivariate analysis identified HLA class I antigen down-regulation as an independent unfavorable prognostic marker. This association is likely to reflect the reduction in the extent of CD8+ T cell infiltration in laryngeal SCC lesions.
Asunto(s)
Carcinoma de Células Escamosas/inmunología , Cisteína Endopeptidasas/biosíntesis , Antígenos HLA-A/biosíntesis , Neoplasias Laríngeas/inmunología , Adulto , Anciano , Anciano de 80 o más Años , Anticuerpos Monoclonales/inmunología , Especificidad de Anticuerpos , Presentación de Antígeno/inmunología , Biomarcadores de Tumor/biosíntesis , Linfocitos T CD8-positivos/inmunología , Carcinoma de Células Escamosas/patología , Regulación hacia Abajo , Femenino , Antígenos HLA-A/inmunología , Humanos , Neoplasias Laríngeas/patología , Masculino , Persona de Mediana Edad , Estadificación de Neoplasias , PronósticoRESUMEN
PURPOSE: Nasal natural killer (NK)/T-cell lymphoma is associated with EBV and has distinct clinical and histologic features. However, little is known about its genetic features. In this study, we examined the genes expressed by SNK-6 and SNT-8 cells, which were established from nasal NK/T-cell lymphomas, and found that interleukin (IL)-9 was specifically expressed in these two cell lines. EXPERIMENTAL DESIGN: cDNA array was used to examine the genes expressed by SNK-6 and SNT-8 cells. Expression of IL-9 and IL-9 receptor was investigated by reverse transcription-PCR, ELISA, and flow cytometry. Cell growth was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Immunohistologic staining and ELISA were used to examine IL-9 expression in biopsies and sera from patients, respectively. RESULTS: In cDNA array, expression of IL-9 mRNA was much higher in SNK-6 and SNT-8 cells than in NK-92 cells from non-nasal NK-cell lymphoma and peripheral blood mononuclear cells from healthy volunteers. Furthermore, IL-9 was specifically expressed by SNK-6 and SNT-8 cells but not by other NK-cell, NK-like T-cell, and T-cell lymphoma/leukemia cell lines. IL-9 receptor was also expressed on the surfaces of SNK-6 and SNT-8 cells. An IL-9-neutralizing antibody inhibited the growth of these two cell lines, whereas recombinant human IL-9 enhanced their growth. Most significantly, IL-9 was present in biopsies and sera from patients with this lymphoma. CONCLUSIONS: These results suggest that IL-9 plays an important role in nasal NK/T-cell lymphoma possibly via an autocrine mechanism.
Asunto(s)
Regulación Neoplásica de la Expresión Génica/fisiología , Interleucina-9/genética , Células Asesinas Naturales/patología , Linfoma de Células T/genética , Neoplasias Nasales/genética , Comunicación Autocrina , Biopsia , Estudios de Casos y Controles , Ensayo de Inmunoadsorción Enzimática , Infecciones por Virus de Epstein-Barr/genética , Infecciones por Virus de Epstein-Barr/patología , Citometría de Flujo , Herpesvirus Humano 4/genética , Humanos , Linfoma de Células T/patología , Linfoma de Células T/virología , Neoplasias Nasales/patología , Neoplasias Nasales/virología , Análisis de Secuencia por Matrices de Oligonucleótidos , ARN Mensajero/metabolismo , Receptores de Interleucina/genética , Receptores de Interleucina-9 , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Células Tumorales CultivadasRESUMEN
To assess the possible negative aspects in the quality of life (QOL) of patients with allergic rhinitis based on the factors such as the symptoms and examination of the nasal cavity, we examined QOL deficits using the Japanese Rhino-conjunctivitis Quality of Life Questionnaire (JRQLQ No1). Thirteen hundred seventy eight allergic rhinitis patients who visited clinics or hospitals between April and July 2003 were assessed for clinical symptoms, had the examination of their nasal cavity graded and answered QOL questionnaires. Gender, period of the disease, antigen, clinical symptoms and the results of the nasal cavity examination were evaluated and analyzed to reveal risk factors associated with negative QOL factors. These negative QOL factors tended to be more severe in women than in men, and more severe in pollen allergy than in house dust mite allergy. There was no association of the period of the disease with negative QOL factors. It was revealed that nasal discharge was the strongest risk factor for problems in usual daily activities, outdoor activities and social functioning in six QOL domains, and nasal congestion was the major factor associated with sleep problems, general physical problems and emotional function. Nasal congestion was also the strongest risk factor in the total score of the QOL questionnaire and the overall face scale of the patients. In the nasal cavity examination, the amount of aqueous secretion was the strongest risk factor for all items associated with negative QOL factors. According to the risk factors we identified, we can treat allergic rhinitis patients taking their QOL into consideration.