RESUMEN

Localization of cytoplasmic messenger RNA transcripts is widely used to target proteins within cells. For many transcripts, localization depends on cis-acting elements within the transcripts and on microtubule-based motors; however, little is known about other components of the transport machinery or how these components recognize specific RNA cargoes. Here, we show that in Drosophila the same machinery and RNA signals drive specific accumulation of maternal RNAs in the early oocyte and apical transcript localization in blastoderm embryos. We demonstrate in vivo that Egalitarian (Egl) and Bicaudal D (BicD), maternal proteins required for oocyte determination, are selectively recruited by, and co-transported with, localizing transcripts in blastoderm embryos, and that interfering with the activities of Egl and BicD blocks apical localization. We propose that Egl and BicD are core components of a selective dynein motor complex that drives transcript localization in a variety of tissues.

Asunto(s)

Proteínas de Drosophila/metabolismo , Drosophila melanogaster/embriología , Oogénesis , ARN Mensajero/metabolismo , Transducción de Señal , Animales , Anticuerpos/inmunología , Transporte Biológico , Blastodermo/metabolismo , Citoplasma/metabolismo , Proteínas de Drosophila/genética , Drosophila melanogaster/genética , Drosophila melanogaster/fisiología , Dineínas/metabolismo , Embrión no Mamífero/metabolismo , Femenino , Microtúbulos/metabolismo , Oocitos/metabolismo , Transporte de Proteínas

RESUMEN

The evolutionarily conserved Ras/mitogen-activated protein kinase (MAPK) cascade is an integral part of the processes of cell division, differentiation, movement and death. Signals received at the cell surface are relayed into the nucleus, where MAPK phosphorylates and thereby modulates the activities of a subset of transcription factors. Here we report the cloning and characterization of a new component of this signal transduction pathway called Mae (for modulator of the activity of Ets). Mae is a signalling intermediate that directly links the MAPK signalling pathway to its downstream transcription factor targets. Phosphorylation by MAPK of the critical serine residue (Ser 127) of the Drosophila transcription factor Yan depends on Mae, and is mediated by the binding of Yan to Mae through their Pointed domains. This phosphorylation is both necessary and sufficient to abrogate transcriptional repression by Yan. Mae also regulates the activity of the transcriptional activator Pointed-P2 by a similar mechanism. Mae is essential for the normal development and viability of Drosophila, and is required in vivo for normal signalling of the epidermal growth factor receptor. Our study indicates that MAPK signalling specificity may depend on proteins that couple specific substrates to the kinase.

Asunto(s)

Proteínas Portadoras/metabolismo , Proteínas de Drosophila , Drosophila melanogaster/metabolismo , Proteínas del Ojo/metabolismo , Proteínas de Insectos/metabolismo , Péptidos y Proteínas de Señalización Intracelular , Sistema de Señalización de MAP Quinasas , Proteínas Quinasas Activadas por Mitógenos/metabolismo , Proteínas Proto-Oncogénicas/metabolismo , Proteínas Represoras/metabolismo , Factores de Transcripción/metabolismo , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Tipificación del Cuerpo , Proteínas Portadoras/química , Proteínas Portadoras/genética , ADN/genética , ADN/metabolismo , Proteínas de Unión al ADN , Drosophila melanogaster/embriología , Drosophila melanogaster/enzimología , Drosophila melanogaster/genética , Receptores ErbB/metabolismo , Proteínas del Ojo/química , Proteínas del Ojo/genética , Regulación de la Expresión Génica , Genes Esenciales/genética , Genes Reporteros/genética , Inmunohistoquímica , Proteínas de Insectos/química , Proteínas de Insectos/genética , Datos de Secuencia Molecular , Proteínas del Tejido Nervioso , Fosforilación , Fosfoserina/metabolismo , Fosfotreonina/metabolismo , Unión Proteica , Estructura Terciaria de Proteína , Proteínas Proto-Oncogénicas/química , Proteínas Proto-Oncogénicas c-ets , Proteínas Represoras/química , Proteínas Represoras/genética , Especificidad por Sustrato , Técnicas del Sistema de Dos Híbridos

RESUMEN

Delta-Notch signalling regulates cell-fate choices in a variety of tissues during development. We report the expression of Delta4 (D14) in arterial endothelium during mouse embryogenesis and in the endothelium of tumor blood vessels. The expression of D14 in the mouse begins at 8 dpc in the dorsal aortae, umbilical artery and the heart. Subsequent expression is restricted to smaller vessels and capillaries and is reduced in most adult tissues. However, it is high in the vasculature of xenograft human tumors in the mouse, in endogenous human tumors and is regulated by hypoxia. These data implicate D14 and the Notch signalling pathway in angiogenesis and suggest possible new targets for antiangiogenic tumor therapy.

Asunto(s)

Proteínas Sanguíneas/fisiología , Endotelio Vascular/fisiología , Sustancias de Crecimiento/fisiología , Péptidos y Proteínas de Señalización Intercelular , Neovascularización Patológica/fisiopatología , Neovascularización Fisiológica/fisiología , Proteínas Adaptadoras Transductoras de Señales , Animales , Arterias/fisiología , Proteínas de Unión al Calcio , Drosophila , Proteínas de Drosophila , Femenino , Humanos , Proteínas de la Membrana/fisiología , Ratones , Ratones Endogámicos BALB C , Especificidad de Órganos , Receptores Notch , Transducción de Señal/fisiología , Células Tumorales Cultivadas

RESUMEN

During Drosophila myogenesis, Notch signalling acts at multiple steps of the muscle differentiation process. In vertebrates, Notch activation has been shown to block MyoD activation and muscle differentiation in vitro, suggesting that this pathway may act to maintain the cells in an undifferentiated proliferative state. In this paper, we address the role of Notch signalling in vivo during chick myogenesis. We first demonstrate that the Notch1 receptor is expressed in postmitotic cells of the myotome and that the Notch ligands Delta1 and Serrate2 are detected in subsets of differentiating myogenic cells and are thus in position to signal to Notch1 during myogenic differentiation. We also reinvestigate the expression of MyoD and Myf5 during avian myogenesis, and observe that Myf5 is expressed earlier than MyoD, consistent with previous results in the mouse. We then show that forced expression of the Notch ligand, Delta1, during early myogenesis, using a retroviral system, has no effect on the expression of the early myogenic markers Pax3 and Myf5, but causes strong down-regulation of MyoD in infected somites. Although Delta1 overexpression results in the complete lack of differentiated muscles, detailed examination of the infected embryos shows that initial formation of a myotome is not prevented, indicating that exit from the cell cycle has not been blocked. These results suggest that Notch signalling acts in postmitotic myogenic cells to control a critical step of muscle differentiation.

Asunto(s)

Drosophila/fisiología , Regulación del Desarrollo de la Expresión Génica/fisiología , Proteínas de la Membrana/fisiología , Músculos/fisiología , Proteína MioD/fisiología , Animales , Drosophila/embriología , Proteínas de Drosophila , Ratones , Músculos/embriología , Receptores Notch , Transducción de Señal/genética

RESUMEN

In vertebrates with mutations in the Notch cell-cell communication pathway, segmentation fails: the boundaries demarcating somites, the segments of the embryonic body axis, are absent or irregular. This phenotype has prompted many investigations, but the role of Notch signalling in somitogenesis remains mysterious. Somite patterning is thought to be governed by a "clock-and-wavefront" mechanism: a biochemical oscillator (the segmentation clock) operates in the cells of the presomitic mesoderm, the immature tissue from which the somites are sequentially produced, and a wavefront of maturation sweeps back through this tissue, arresting oscillation and initiating somite differentiation. Cells arrested in different phases of their cycle express different genes, defining the spatially periodic pattern of somites and controlling the physical process of segmentation. Notch signalling, one might think, must be necessary for oscillation, or to organize subsequent events that create the somite boundaries. Here we analyse a set of zebrafish mutants and arrive at a different interpretation: the essential function of Notch signalling in somite segmentation is to keep the oscillations of neighbouring presomitic mesoderm cells synchronized.

Asunto(s)

Tipificación del Cuerpo/fisiología , Proteínas de la Membrana/fisiología , Transducción de Señal , Animales , Tipificación del Cuerpo/genética , Embrión no Mamífero/citología , Embrión no Mamífero/fisiología , Desarrollo Embrionario , Péptidos y Proteínas de Señalización Intracelular , Ligandos , Proteínas de la Membrana/biosíntesis , Mesodermo/fisiología , Mutación , Receptores Notch , Somitos/fisiología , Pez Cebra

RESUMEN

Somitic segmentation provides the framework on which the segmental pattern of the vertebrae, some muscles and the peripheral nervous system is established. Recent evidence indicates that a molecular oscillator, the 'segmentation clock', operates in the presomitic mesoderm (PSM) to direct periodic expression of c-hairy1 and lunatic fringe (l-fng). Here, we report the identification and characterisation of a second avian hairy-related gene, c-hairy2, which also cycles in the PSM and whose sequence is closely related to the mammalian HES1 gene, a downstream target of Notch signalling in vertebrates. We show that HES1 mRNA is also expressed in a cyclic fashion in the mouse PSM, similar to that observed for c-hairy1 and c-hairy2 in the chick. In HES1 mutant mouse embryos, the periodic expression of l-fng is maintained, suggesting that HES1 is not a critical component of the oscillator mechanism. In contrast, dynamic HES1 expression is lost in mice mutant for Delta1, which are defective for Notch signalling. These results suggest that Notch signalling is required for hairy-like genes cyclic expression in the PSM.

Asunto(s)

Proteínas Aviares , Proteínas de Homeodominio , Proteínas de la Membrana/metabolismo , Mesodermo/metabolismo , Proteínas Musculares/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico , Relojes Biológicos/genética , Tipificación del Cuerpo/genética , Embrión de Pollo , Clonación Molecular , Cartilla de ADN/genética , Regulación del Desarrollo de la Expresión Génica , Péptidos y Proteínas de Señalización Intracelular , Proteínas de la Membrana/genética , Ratones , Ratones Noqueados , Datos de Secuencia Molecular , Proteínas/genética , ARN Mensajero/genética , ARN Mensajero/metabolismo , Receptores Notch , Homología de Secuencia de Aminoácido , Transducción de Señal , Somitos/metabolismo , Factor de Transcripción HES-1

RESUMEN

Signals derived from antigen-presenting cells (APC) influence the functional differentiation of CD4(+) T cells. We report here that Serrate1 (Jagged1), a ligand for the Notch1 receptor, may contribute to the differentiation of peripheral CD4(+) T cells into either helper or regulatory cells. Our findings demonstrate that antigen presented by murine APC overexpressing human Serrate1 induces naive peripheral CD4(+) T cells to become regulatory cells. These cells can inhibit primary and secondary immune responses, and transfer antigen-specific tolerance to recipient mice. Our results show that Notch signalling may help explain 'linked' suppression in peripheral tolerance, whereby tolerance induced to one epitope encompasses all epitopes on that antigen during the course of an immune response.

Asunto(s)

Linfocitos T CD4-Positivos/inmunología , Tolerancia Inmunológica , Proteínas de la Membrana/metabolismo , Transducción de Señal , Animales , Células Presentadoras de Antígenos/inmunología , Células Presentadoras de Antígenos/metabolismo , Antígenos Dermatofagoides , Proteínas de Unión al Calcio , Células Cultivadas , Epítopos/inmunología , Femenino , Regulación de la Expresión Génica/inmunología , Glicoproteínas/inmunología , Humanos , Inmunidad Celular , Inmunización , Péptidos y Proteínas de Señalización Intercelular , Proteína Jagged-1 , Leucocitos Mononucleares/inmunología , Proteínas de la Membrana/genética , Ratones , Ovalbúmina/inmunología , Ratas , Receptores Notch , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Proteínas Serrate-Jagged

RESUMEN

Drosophila melanogaster pair-rule segmentation gene transcripts localize apically of nuclei in blastoderm embryos. This might occur by asymmetric (vectorial) export from one side of the nucleus or by transport within the cytoplasm. We have followed fluorescently labeled pair-rule transcripts postinjection into Drosophila embryos. Naked, microinjected fushi tarazu (ftz) transcripts do not localize in blastoderm embryos, indicating that cytoplasmic mechanisms alone are insufficient for apical targeting. However, prior exposure of ftz to Drosophila or human embryonic nuclear extract leads to rapid, specific, microtubule-dependent transport, arguing against vectorial export. We present evidence that ftz transcript localization involves the Squid (Hrp40) hnRNP protein and that the activity of hnRNP proteins in promoting transcript localization is evolutionarily conserved. We propose that cytoplasmic localization machineries recognize transcripts in the context of nuclear partner proteins.

Asunto(s)

Proteínas de Drosophila , Drosophila melanogaster/genética , Proteínas de Homeodominio/genética , Hormonas de Insectos/metabolismo , Proteínas de Unión al ARN/metabolismo , Regiones no Traducidas 3'/fisiología , Citoesqueleto de Actina/fisiología , Animales , Transporte Biológico/efectos de los fármacos , Transporte Biológico/fisiología , Blastodermo/metabolismo , Reactivos de Enlaces Cruzados , Citoplasma/metabolismo , Drosophila melanogaster/crecimiento & desarrollo , Embrión no Mamífero/metabolismo , Evolución Molecular , Femenino , Factores de Transcripción Fushi Tarazu , Regulación del Desarrollo de la Expresión Génica , Microinyecciones , Microtúbulos/fisiología , Proteínas Nucleares/farmacología , Ovario/química , Unión Proteica/fisiología , ARN Mensajero/farmacocinética , Transcripción Genética/fisiología , Rayos Ultravioleta

RESUMEN

A novel Drosophila melanogaster gene UBL3 was characterized and shown to be highly conserved in man and Caenorhabditis elegans (C. elegans). The human and mouse homologues were cloned and sequenced. UBL3 is a ubiquitin-like protein of unknown function with no conserved homologues in yeast. Mapping of the human and mouse UBL3 genes places them within a region of shared gene order between human and mouse chromosomes on human chromosome 13q12-13 and telomeric mouse chromosome 5 (MMU5).

Asunto(s)

Proteínas de Drosophila , Proteínas de Insectos/genética , Secuencia de Aminoácidos , Animales , Mapeo Cromosómico , Clonación Molecular , ADN Complementario , Drosophila melanogaster/genética , Humanos , Datos de Secuencia Molecular , Homología de Secuencia de Aminoácido

RESUMEN

Surprisingly small peptide motifs can confer critical biological functions. One example is the WRPW tetrapeptide present in the Hairy family of transcriptional repressors, which mediates recruitment of the Groucho (Gro) corepressor to target promoters. We recently showed that Engrailed (En) is another repressor that requires association with Gro for its function. En lacks a WRPW motif; instead, it contains another short conserved sequence, the En homology region 1 (eh1)/GEH motif, that is likely to play a role in tethering Gro to the promoter. Here, we characterize a repressor domain from the Goosecoid (Gsc) developmental regulator that includes an eh1/GEH-like motif. We demonstrate that this domain (GscR) mediates efficient repression in Drosophila blastoderm embryos and that repression by GscR requires Gro function. GscR and Gro interact in vitro, and the eh1/GEH motif is necessary and sufficient for the interaction and for in vivo repression. Because WRPW- and eh1/GEH-like motifs are present in different proteins and in many organisms, the results suggest that interactions between short peptides and Gro represent a widespread mechanism of repression. Finally, we investigate whether Gro is part of a stable multiprotein complex in the nucleus. Our results indicate that Gro does not form stable associations with other proteins but that it may be able to assemble into homomultimeric complexes.

Asunto(s)

Proteínas de Unión al ADN/metabolismo , Regulación del Desarrollo de la Expresión Génica , Proteínas de Homeodominio/metabolismo , Proteínas Represoras/metabolismo , Factores de Transcripción , Animales , Animales Modificados Genéticamente , Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico , Sitios de Unión , Núcleo Celular/metabolismo , Proteínas de Unión al ADN/genética , Drosophila/embriología , Drosophila/genética , Proteínas de Drosophila , Proteína Goosecoide , Proteínas de Homeodominio/genética , Proteínas Nucleares/metabolismo , Proteínas Represoras/genética

RESUMEN

The sensory patches in the vertebrate inner ear are similar in function to the mechanosensory bristles of a fly, and consist of a similar set of cell types. If they are truly homologous structures, they should also develop by similar mechanisms. We examine the genesis of the neurons, hair cells and supporting cells that form the sensory patches in the inner ear of the chick. These all arise from the otic epithelium, and are produced normally even in otic epithelium cultured in isolation, confirming that their production is governed by mechanisms intrinsic to the epithelium. First, the neuronal sublineage becomes separate from the epithelial: between E2 and E3.5, neuroblasts delaminate from the otocyst. The neuroblasts then give rise to a mixture of neurons and neuroblasts, while the sensory epithelial cells diversify to form a mixture of hair cells and supporting cells. The epithelial patches where this occurs are marked from an early stage by uniform and maintained expression of the Notch ligand Serrate1. The Notch ligand Delta1 is also expressed, but transiently and in scattered cells: it is seen both early, during neuroblast segregation, where it appears to be in the nascent neuroblasts, and again later, in the ganglion and in differentiating sensory patches, where it appears to be in the nascent hair cells, disappearing as they mature. Delta-Notch-mediated lateral inhibition may thus act at each developmental branchpoint to drive neighbouring cells along different developmental pathways. Our findings indicate that the sensory patches of the vertebrate inner ear and the sensory bristles of a fly are generated by minor variations of the same basic developmental program, in which cell diversification driven by Delta-Notch and/or Serrate-Notch signalling plays a central part.

Asunto(s)

Oído Interno/embriología , Embrión no Mamífero/fisiología , Mecanorreceptores/embriología , Proteínas de la Membrana/genética , Neuronas/citología , Animales , Proteínas de Unión al Calcio , Diferenciación Celular , Células Cultivadas , Drosophila/embriología , Proteínas de Drosophila , Oído Interno/citología , Embrión no Mamífero/citología , Inducción Embrionaria , Células Epiteliales/citología , Epitelio/embriología , Regulación del Desarrollo de la Expresión Génica , Células Ciliadas Auditivas/citología , Células Ciliadas Auditivas/embriología , Péptidos y Proteínas de Señalización Intercelular , Péptidos y Proteínas de Señalización Intracelular , Proteína Jagged-1 , Mecanorreceptores/citología , Neuronas/clasificación , Neuronas/fisiología , Receptores Notch , Proteínas Serrate-Jagged

RESUMEN

Little is known about the tissue interactions and the molecular signals implicated in the sequence of events leading to the subdivision of the somite into its rostral and caudal compartments. It has been demonstrated that rostrocaudal identity of the sclerotome is acquired at the presomitic (PSM) level. However, it is not known whether this compartment specification is fully determined in the PSM or whether it is dependent upon maintenance cues from the surrounding environment, as is the case for somite epithelialization. In this report, we address this issue by examining the expression profiles of C-Delta-1 and C-Notch-1, the avian homologues of mouse Delta-like1 (Delta1) and Notch1 which have been implicated in the specification of the somite rostrocaudal polarity in mouse. In chick, these genes are expressed in distinct but partially overlapping domains in the PSM and subsequently in the caudal regions of the somites. We have used an in vitro assay that consists of culturina PSM explants to examine the regulation of these genes in this tissue. We find that PSM explants cultured without overlying ectoderm continue to lay down stripes of C-Delta-1 expression, although epithelialization is blocked. These results suggest that somite rostrocaudal patterning is an autonomous property of the PSM. In addition, they demonstrate that segmentation is not necessarily coupled with the formation of somites.

Asunto(s)

Proteínas Aviares , Tipificación del Cuerpo/genética , Mesodermo/citología , Receptores de Superficie Celular , Somitos/citología , Factores de Transcripción , Animales , Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico , Embrión de Pollo , Ectodermo/citología , Regulación del Desarrollo de la Expresión Génica , Hibridación in Situ , Técnicas In Vitro , Péptidos y Proteínas de Señalización Intracelular , Proteínas de la Membrana/genética , Ratones , Proteínas/genética , Receptor Notch1

RESUMEN

Chick embryonic feather buds arise in a distinct spatial and temporal pattern. Although many genes are implicated in the growth and differentiation of the feather buds, little is known about how the discrete pattern of the feather array is formed and which gene products may be involved. Possible candidates include Notch and its ligands, Delta and Serrate, as they play a role in numerous cell fate decisions in many organisms. Here we show that Notch-1 and Notch-2 mRNAs are expressed in the skin in a localized pattern prior to feather bud initiation. In the early stages of feather bud development, Delta-1 and Notch-1 are localized to the forming buds while Notch-2 expression is excluded from the bud. Thus, Notch and Delta-1 are expressed at the correct time and place to be players in the formation of the feather pattern. Once the initial buds form, expression of Notch and its ligands is observed within each bud. Notch-1 and -2 and Serrate-1 and -2 are expressed throughout the growth and differentiation of the feathers whereas Delta-1 transcripts are downregulated. We have also misexpressed chick Delta-1 using a replication competent retrovirus. This results in induction of Notch-1 and-2 and a loss of feather buds from the embryo in either large or small patches. In large regions of Delta-1 misexpression, feathers are lost throughout the infected area. In contrast, in small regions of misexpression, Delta-1 expressing cells differentiate into feather buds more quickly than normal and inhibit their neighbors from accepting a feather fate. We propose a dual role for Delta-1 in promoting feather bud development and in lateral inhibition. These results implicate the Notch/Delta receptor ligand pair in the formation of the feather array.

Asunto(s)

Plumas/embriología , Proteínas de la Membrana/genética , Receptores de Superficie Celular/genética , Factores de Transcripción , Animales , Proteínas de Unión al Calcio , Diferenciación Celular/genética , Embrión de Pollo , Plumas/citología , Plumas/efectos de los fármacos , Factores de Crecimiento de Fibroblastos/farmacología , Regulación del Desarrollo de la Expresión Génica/efectos de los fármacos , Hibridación in Situ , Péptidos y Proteínas de Señalización Intercelular , Péptidos y Proteínas de Señalización Intracelular , Proteínas de la Membrana/fisiología , ARN Mensajero/genética , ARN Mensajero/metabolismo , Receptor Notch1 , Receptor Notch2 , Receptores de Superficie Celular/fisiología , Proteínas Serrate-Jagged , Transducción de Señal

RESUMEN

Relatively little is known about the molecular mechanisms involved in transcriptional repression, despite its importance in development and differentiation. Recent evidence suggests that some transcriptional repressors act by way of adaptor molecules known as corepressors. Here, we use in vivo functional assays to test whether different repressor activities are mediated by the Groucho (Gro) corepressor in the Drosophila embryo. Previously, Gro was proposed to mediate repression by the Hairy-related family of basic helix-loop-helix proteins. Our results indicate not only that repression by Hairy requires Gro, but that a repressor domain from the Engrailed (En) homeodomain protein is also Gro dependent. The latter result correlates with an ability of this En domain to bind to Gro in vitro. In contrast, repressor regions from the Even-skipped, Snail, Krüppel, and Knirps transcription factors are effective in the absence of Gro. These results show that Gro is not generally required for repression, but acts as a specific corepressor for a fraction of negative regulators, including Hairy and En.

Asunto(s)

Proteínas Bacterianas , Proteínas de Unión al ADN/fisiología , Proteínas de Drosophila , Drosophila melanogaster/genética , Regulación del Desarrollo de la Expresión Génica , Proteínas de Homeodominio/fisiología , Proteínas de Insectos/fisiología , Proteínas Represoras/fisiología , Factores de Transcripción , Animales , Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico , Drosophila melanogaster/embriología , Secuencias Hélice-Asa-Hélice , Sustancias Macromoleculares , Unión Proteica , Proteínas de Unión al ARN/genética , Proteínas Recombinantes de Fusión

RESUMEN

We have identified and characterized c-hairy1, an avian homolog of the Drosophila segmentation gene, hairy. c-hairy1 is strongly expressed in the presomitic mesoderm, where its mRNA exhibits cyclic waves of expression whose temporal periodicity corresponds to the formation time of one somite (90 min). The apparent movement of these waves is due to coordinated pulses of c-hairy1 expression, not to cell displacement along the anteroposterior axis, nor to propagation of an activating signal. Rather, the rhythmic c-hairy mRNA expression is an autonomous property of the paraxial mesoderm. These results provide molecular evidence for a developmental clock linked to segmentation and somitogenesis of the paraxial mesoderm, and support the possibility that segmentation mechanisms used by invertebrates and vertebrates have been conserved.

Asunto(s)

Proteínas Aviares , Relojes Biológicos/genética , Proteínas/genética , Somitos/fisiología , Animales , Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico , Movimiento Celular/genética , Embrión de Pollo , Cicloheximida , Drosophila/genética , Regulación del Desarrollo de la Expresión Génica/fisiología , Mesodermo/citología , Mesodermo/fisiología , Datos de Secuencia Molecular , Periodicidad , Inhibidores de la Síntesis de la Proteína , ARN Mensajero/análisis , Homología de Secuencia de Aminoácido , Somitos/citología , Vertebrados

RESUMEN

Patterning of the non-segmental termini of the Drosophila embryo depends on signalling via the Torso receptor tyrosine kinase (RTK). Activation of Torso at the poles of the embryo triggers restricted expression of the zygotic gap genes tailless (tll) and huckebein (hkb). In this paper, we show that the Groucho (Gro) corepressor acts in this process to confine terminal gap gene expression to the embryonic termini. Embryos lacking maternal gro activity display ectopic tll and hkb transcription; the former leads, in turn, to lack of abdominal expression of the Krüppel and knirps gap genes. We show that torso signalling permits terminal gap gene expression by antagonising Gro-mediated repression. Thus, the corepressor Gro is employed in diverse developmental contexts and, probably, by a variety of DNA-binding repressors.

Asunto(s)

Tipificación del Cuerpo , Proteínas de Unión al ADN/metabolismo , Proteínas de Drosophila , Drosophila/embriología , Embrión no Mamífero/fisiología , Proteínas Tirosina Quinasas Receptoras/fisiología , Proteínas Represoras/metabolismo , Abdomen , Animales , Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico , Cruzamientos Genéticos , Proteínas de Unión al ADN/biosíntesis , Embrión no Mamífero/citología , Femenino , Regulación del Desarrollo de la Expresión Génica , Genes de Insecto , Hormonas de Insectos/biosíntesis , Masculino , Mutagénesis , Proteínas Represoras/biosíntesis , Transducción de Señal , Dedos de Zinc , Cigoto/fisiología

RESUMEN

BACKGROUND: Neurons of the vertebrate central nervous system (CNS) are generated sequentially over a prolonged period from dividing neuroepithelial progenitor cells. Some cells in the progenitor cell population continue to proliferate while others stop dividing and differentiate as neurons. The mechanism that maintains the balance between these two behaviours is not known, although previous work has implicated Delta-Notch signalling in the process. RESULTS: In normal development, the proliferative layer of the neuroepithelium includes both nascent neurons that transiently express Delta-1 (Dl1), and progenitor cells that do not. Using retrovirus-mediated gene misexpression in the embryonic chick retina, we show that where progenitor cells are exposed to Dl1 signalling, they are prevented from embarking on neuronal differentiation. A converse effect is seen in cells expressing a dominant-negative form of Dl1, Dl1(dn), which we show renders expressing cells deaf to inhibitory signals from their neighbours. In a multicellular patch of neuroepithelium expressing Dl1(dn), essentially all progenitors stop dividing and differentiate prematurely as neurons, which can be of diverse types. Thus, Delta-Notch signalling controls a cell's choice between remaining as a progenitor and differentiating as a neuron. CONCLUSIONS: Nascent retinal neurons, by expressing Dl1, deliver lateral inhibition to neighbouring progenitors; this signal is essential to prevent progenitors from entering the neuronal differentiation pathway. Lateral inhibition serves the key function of maintaining a balanced mixture of dividing progenitors and differentiating progeny. We propose that the same mechanism operates throughout the vertebrate CNS, enabling large numbers of neurons to be produced sequentially and adopt different characters in response to a variety of signals. A similar mechanism of lateral inhibition, mediated by Delta and Notch proteins, may regulate stem-cell function in other tissues.

Asunto(s)

Proteínas de la Membrana/fisiología , Neuronas/citología , Receptores de Superficie Celular/fisiología , Retina/citología , Transducción de Señal , Células Madre/citología , Factores de Transcripción , Animales , Diferenciación Celular/fisiología , División Celular , Embrión de Pollo , Péptidos y Proteínas de Señalización Intracelular , Morfogénesis , Receptor Notch1 , Retina/embriología

RESUMEN

Drosophila melanogaster neurogenesis requires the opposing activities of two sets of basic helix-loop-helix (bHLH) proteins: proneural proteins, which confer on cells the ability to become neural precursors, and the Enhancer-of-split [E(spl)] proteins, which restrict such potential as part of the lateral inhibition process. Here, we test if E(spl) proteins function as promoter-bound repressors by examining the effects on neurogenesis of an E(spl) derivative containing a heterologous transcriptional activation domain [E(spl) m7Act (m7Act)]. In contrast to the wild-type E(spl) proteins, m7Act efficiently induces neural development, indicating that it binds to and activates target genes normally repressed by E(spl). Mutations in the basic domain disrupt m7Act activity, suggesting that its effects are mediated through direct DNA binding. m7Act causes ectopic transcription of the proneural achaete and scute genes. Our results support a model in which E(spl) proteins normally regulate neurogenesis by direct repression of genes at the top of the neural determination pathway.

Asunto(s)

Proteínas de Unión al ADN/genética , Proteínas de Drosophila , Drosophila melanogaster/genética , Regulación del Desarrollo de la Expresión Génica/genética , Proteínas de Insectos/genética , Transactivadores/genética , Animales , Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico , Proteínas de Unión al ADN/fisiología , Drosophila melanogaster/crecimiento & desarrollo , Secuencias Hélice-Asa-Hélice , Proteínas de Insectos/fisiología , Masculino , Mutación , Sistema Nervioso/crecimiento & desarrollo , Fenotipo , Proteínas Recombinantes de Fusión , Proteínas Represoras/genética , Proteínas Represoras/fisiología , Transactivadores/fisiología , Factores de Transcripción/genética , Alas de Animales/crecimiento & desarrollo

RESUMEN

In vertebrate embryos, the precursor cells of the central nervous system (CNS) are induced by signaling from the organizer region. Here we report the isolation of a novel vertebrate achaete-scute homolog, cash4, which is expressed in the presumptive posterior nervous system in response to such signaling. cash4 is first expressed in epiblast cells flanking the late-phase organizer (Hensen's node), which retains its ability to induce cash4 during regression to the caudal end of the embryo. We show that these node-derived signals can be mimicked in vivo by the activity of fibroblast growth factor (FGF). We demonstrate that cash4 can substitute for the achaete/scute genes in the fly and that it also has proneural activity in vertebrate embryos. Together these results suggest that cash4 functions as a proneural gene downstream of node-derived signals (including FGF) to promote the formation of the neural precursors that will give rise to the posterior CNS in the chick embryo.

Asunto(s)

Proteínas Aviares , Sistema Nervioso Central/embriología , Embrión de Pollo/fisiología , Proteínas de Unión al ADN/genética , Proteínas de Drosophila , Factores de Transcripción/genética , Secuencia de Aminoácidos , Anfibios/embriología , Anfibios/genética , Animales , Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico , Diferenciación Celular/genética , Embrión de Pollo/citología , Clonación Molecular , ADN Complementario , Proteínas de Unión al ADN/efectos de los fármacos , Proteínas de Unión al ADN/metabolismo , Drosophila/embriología , Drosophila/genética , Embrión no Mamífero/fisiología , Inducción Embrionaria/genética , Desarrollo Embrionario y Fetal/genética , Factores de Crecimiento de Fibroblastos/farmacología , Regulación del Desarrollo de la Expresión Génica/efectos de los fármacos , Datos de Secuencia Molecular , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Homología de Secuencia de Aminoácido , Factores de Transcripción/efectos de los fármacos , Factores de Transcripción/metabolismo