RESUMEN
Six Ligularia fischeri samples, two from Sichuan (samples 1 and 2) and four from Chongqing (samples 3-6), were examined for root chemicals and the DNA sequence of the internal transcribed spacers of the ribosomal RNA gene. Samples 2 and 3 contained benzofurans. The isolation of benzofurans shows that the chemical diversity in L. fischeri is higher than previously reported. Samples 1, 4, 5, and 6 contained eremophilanes. However, the compounds were different between sample 1 and samples 4-6, indicating variation within eremophilane producers. DNA data indicated that introgression could be a mechanism of benzofuran production in sample 2 and that sample 1 and samples 4-6 were genetically separate.
Asunto(s)
Asteraceae/metabolismo , Proteínas de Plantas/química , Proteínas de Plantas/metabolismo , Secuencia de Aminoácidos , Asteraceae/química , Asteraceae/genética , Secuencia de Bases , China , ADN Espaciador Ribosómico/genética , Demografía , Regulación de la Expresión Génica de las Plantas/fisiología , Estructura Molecular , Filogenia , Proteínas de Plantas/genética , Especificidad de la EspecieRESUMEN
Ligularia hodgsonii was found to be diverse in China. Furanoeremophilanes were isolated from samples collected in Yunnan Province, while such compounds were absent from samples from Sichuan, Gansu, and Chongqing. DNA sequencing showed that the Yunnan samples were also genetically distinct. γ-Humulene and a new bisabolane sesquiterpene were isolated.
Asunto(s)
Asteraceae/química , Asteraceae/genética , Variación Genética , Extractos Vegetales/química , Sesquiterpenos/química , China , Estructura Molecular , Sesquiterpenos Monocíclicos , Extractos Vegetales/aislamiento & purificación , Sesquiterpenos/aislamiento & purificaciónRESUMEN
Ligularia vellerea and L. melanothyrsa have been found to share chemical characteristics based on geography. In one chemotype (Shangrila type), the major components were either 15-oxygenated furanoeremophilanes or eremophilanolides; and in another (Luguhu type), either 1-angeloyloxyfuranoeremophilanes or corresponding lactones. The two L. vellerea samples were found to have experienced hybridization with either L. cyathiceps or L. kanaitzensis; however, compounds typical of L. vellerea alone were isolated from them.