1.
Biomed Opt Express
; 13(2): 824-837, 2022 Feb 01.
Artículo
en Inglés
| MEDLINE
| ID: mdl-35284170
RESUMEN
The parallel-plate compression test is one of the simplest ways to measure the mechanical properties of a material. In this test, the Young's modulus ( E ) and the Poisson's ratio ( ν ) of the material are determined directly without applying any additional modelling and parameter fitting in the post-processing. This is, however, limited when dealing soft biological materials due to their inherent properties such as being inhomogeneous, microscopic, and overly compliant. By combining an interferometry-assisted parallel-plate compression system and a confocal microscope, we were able to overcome these limitations and measure the E (315 ± 52 Pa) and ν (0.210 ± 0.043) of fixated and permeabilized bovine oocytes.