RESUMEN
Fabrication of engineered intestinal tissues with the structures and functions as humans is crucial and promising as the tools for developing drugs and functional foods. The aim of this study is to fabricate an engineered intestinal tissue from Caco-2 cells by air-liquid interface culture using a paper-based dual-layer scaffold and analyze its structure and functions. Just by simply placing on a folded paper soaked in the medium, the electrospun gelatin microfiber mesh as the upper cell adhesion layer of the dual-layer scaffold was exposed to the air, while the lower paper layer worked to preserve and supply the cell culture medium to achieve stable culture over several weeks. Unlike the flat tissue produced using the conventional commercial cultureware, Transwell, the engineered intestinal tissue fabricated in this study formed three-dimensional villous architectures. Microvilli and tight junction structures characteristic of epithelial tissue were also formed at the apical side. Furthermore, compared to the tissue prepared by Transwell, mucus production was significantly larger, and the enzymatic activities of drug metabolism and digestion were almost equivalent. In conclusion, the air-liquid interface culture using the paper-based dual-layer scaffold developed in this study was simple but effective in fabricating the engineered intestinal tissue with superior structures and functions.
Asunto(s)
Moco , Papel , Ingeniería de Tejidos , Andamios del Tejido , Andamios del Tejido/química , Humanos , Células CACO-2 , Moco/metabolismo , Intestinos/citología , Intestinos/fisiología , Mucosa Intestinal/metabolismo , Mucosa Intestinal/citología , Aire , Técnicas de Cultivo de Célula/métodosRESUMEN
An 8-month-old boy with a left-sided incarcerated inguinal hernia involving the appendix, cecum, and terminal ileum was successfully managed via an inguinal approach during an emergency operation. A mobile cecum seemed to have contributed to the left-sided incarceration. Only 13 similar cases with the left-sided Amyand's hernia have been reported in the literature.
Asunto(s)
Anomalías Múltiples/diagnóstico , Aorta Torácica/anomalías , Costilla Cervical , Conducto Arterioso Permeable/diagnóstico , Atresia Esofágica/diagnóstico , Anemia de Fanconi/diagnóstico , Retardo del Crecimiento Fetal/diagnóstico , Anomalías Múltiples/genética , Trasplante de Células Madre de Sangre del Cordón Umbilical , Daño del ADN , Análisis Mutacional de ADN , Conducto Arterioso Permeable/genética , Atresia Esofágica/genética , Anemia de Fanconi/genética , Proteínas del Grupo de Complementación de la Anemia de Fanconi/genética , Resultado Fatal , Retardo del Crecimiento Fetal/genética , Estudios de Seguimiento , Humanos , Lactante , Recién Nacido , Masculino , Fenotipo , Transducción de Señal/genéticaRESUMEN
We present a pediatric case of neurofibromatosis-1 (NF-1) complicated by acute lymphoblastic leukemia and hypereosinophilia, which caused multiple end-organ damage. Although clinical symptoms such as fever and coughing were noted only 1 week before admission, the condition deteriorated rapidly with a fatal outcome prior to antileukemic therapy. A postmortem examination demonstrated extensive endomyocardial fibrosis with thrombotic occlusion and recanalization of the coronary arteries. Leukemic cell infiltration was not seen in the cardiac tissue. When eosinophilia is diagnosed in patients with NF-1, eosinophilic end-organ damage, particularly cardiac involvement, in addition to hematological malignancies, should be screened for in order to start medical treatment at the early stage of the disease.