Asunto(s)
Contaminación Ambiental/efectos adversos , Enfermedades Genéticas Congénitas/etiología , Contaminantes del Suelo/efectos adversos , Contaminantes del Agua/efectos adversos , ADN/genética , Contaminación Ambiental/análisis , Enfermedades Genéticas Congénitas/diagnóstico , Humanos , Kazajstán , Mutación , Contaminantes del Suelo/análisis , Población Urbana , Contaminantes del Agua/análisisAsunto(s)
Anticuerpos Antinucleares/genética , Daño del ADN/genética , ADN/genética , Lupus Eritematoso Sistémico/genética , Linfocitos/inmunología , Neutrófilos/inmunología , Anticuerpos Antinucleares/sangre , ADN/inmunología , Daño del ADN/inmunología , Humanos , Lupus Eritematoso Sistémico/sangre , Lupus Eritematoso Sistémico/inmunología , Estructura MolecularRESUMEN
The present study was made to determine the genotoxicity of psychoemotional stress (PES) alone and its possible influence on chemical mutagenesis under combined treatment. Stress was registered by the classical parameters: 1) weights of the thymus and adrenal; 2) stomach and duodenal mucosa conditions. The unscheduled DNA synthesis (UDS) measurement in peripheral blood lymphocytes with simultaneous determination of chromosomal aberrations (CA) and micronuclei (MN) levels in bone marrow cells were applied for estimation the mutagenic activity. The results indicated that PES alone, as CP, induced UDS in peripheral blood lymphocytes. Intensity of UDS was dependent on the stress duration. The UDS kinetics in the lymphocytes from stressed mice and stressed mice treated with CP were different. PES and CP induced the similar amounts of CA in bone marrow cells. Value of CA had a comparable tendency with results of UDS. The combined effect of PES and CP in both tests was different from additive one. The effects in NM test were statistically significant only in case of combined PES and CP action. The mutagenic potency of PES and its role in the modification of environmental mutagenesis will be discussed.
Asunto(s)
Mutagénesis/genética , Mutación/genética , Estrés Psicológico/genética , Animales , Enfermedad Crónica , Ciclofosfamida/toxicidad , Genes/efectos de los fármacos , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos CBA , Factores de TiempoRESUMEN
It has been found that in 35-40% of patients with acute and chronic pneumonia, the reduced ability of peripheral blood lymphocytes for DNA repair is encountered 14 times as frequently as in normal subjects. After discontinuation of antibacterial therapy with penicillin or tetracycline antibiotics marked inhibition of the ability of lymphocytes for DNA repair is recorded. The tendency toward normalization of this characteristic occurs 1 week after discontinuation of antibiotic therapy for acute and 3 weeks for chronic pneumonia. The disorders of the ability of peripheral blood lymphocytes for DNA repair in the conditions under study are regarded as a possible cause of secondary immunodeficiency.