RESUMEN
Here, we present new antimicrobial nanoparticles based on silica nanoparticles (SNPs) coated with a quaternary ammonium cationic surfactant, didodecyldimethylammonium bromide (DDAB). Depending on the initial concentration of DDAB, SNPs immobilize between 45 and 275 µg of DDAB per milligram of nanoparticle. For high concentrations of DDAB adsorbed to SNP, a bilayer is formed as confirmed by zeta potential measurements, thermogravimetry, and diffuse reflectance infrared Fourier transform (DRIFT) analyses. Interestingly, these nanoparticles have lower minimal inhibitory concentrations (MIC) against bacteria and fungi than soluble surfactant. The electrostatic interaction of the DDAB with the SNP is strong, since no measurable loss of antimicrobial activity was observed after suspension in aqueous solution for 60 days. We further show that the antimicrobial activity of the nanoparticle does not require the leaching of the surfactant from the surface of the NPs. The SNPs may be immobilized onto surfaces with different chemistry while maintaining their antimicrobial activity, in this case extended to a virucidal activity. The versatility, relative facility in preparation, low cost, and large antimicrobial activity of our platform makes it attractive as a coating for large surfaces.
Asunto(s)
Antifúngicos/farmacología , Nanopartículas/química , Dióxido de Silicio/química , Dióxido de Silicio/farmacología , Antibacterianos/química , Antibacterianos/farmacología , Antifúngicos/química , Antivirales/química , Antivirales/farmacología , Candida albicans/efectos de los fármacos , Escherichia coli/efectos de los fármacos , Subtipo H1N1 del Virus de la Influenza A/efectos de los fármacos , Pruebas de Sensibilidad Microbiana , Compuestos de Amonio Cuaternario/química , Staphylococcus aureus/efectos de los fármacos , Propiedades de Superficie , Tensoactivos/química , Factores de Tiempo , Agua/químicaRESUMEN
Films of pseudoisocyanine iodide in a cellulose acetate matrix were prepared by spin coating and characterized by UV/Vis absorption and fluorescence spectroscopies. The comparison with self-supported films of the same dye enabled analysing the role of the matrix in the aggregation of pseudoisocyanine iodide ([PIC]I). It was proved that cellulose acetate is a suitable support for [PIC]I J-aggregates, which form during spinning, as shown by a very sharp J-band in the absorption spectra. This indicates a perfect coherence between stacked monomers in the supported J-aggregates. It was possible to individualize the emission spectrum of [PIC]I J-aggregates in cellulose acetate, by decomposition of the steady-state fluorescence spectra of the films. The dependence on the excitation wavelength of the relative emission intensities of monomers and J-aggregates, for lambda(em) = 587 nm, lead to confirm that the latter species have an absorption maximum at approximately 500 nm in cellulose acetate. Finally, polarised absorption spectra of films obtained by the vertical spin coating technique showed that cellulose acetate allows a partial orientation of J-aggregates.
Asunto(s)
Celulosa/análogos & derivados , Celulosa/química , Colorantes/química , Quinolinas/química , Estructura Molecular , Yoduro de Potasio/química , Espectrometría de Fluorescencia , Espectrofotometría UltravioletaRESUMEN
The symmetric (v1) and antisymmetric (v3) stretching bands of water monomers in toluene are revisited using two approaches: (i) calculation of dipole autocorrelation functions (ii) the solvatochromic behaviour of both vibration frequency shifts. The time constants extracted from the autocorrelations account for meaningful differences between the couplings established by the antisymmetric and symmetric vibrations with the solvent. The dipole autocorrelation function for the symmetric stretching band fits well a Lorentzian spectral density and shows a higher contribution to hindered rotation relatively to the one obtained from the antisymmetric stretching. The spectral shifts of stretching frequencies in toluene and in other relevant solvents were interpreted as arising from the electronic and orientational polarisations. Characteristic donor/acceptor interactions also contribute to the red shift and were tested by using empirical solvent basicity scales such as Kamlet-Taft beta and the recently proposed SB. The deviations detected in toluene as regards the continuum dielectric predictions are quantitatively treated and account for the specific interaction between the water and the molecular pi electron system referred to in the literature.