RESUMEN
The synthesis of copper nanoparticles (CuNPs) using Wrightia tinctoria (Wt) R.Br extract is defined in this article as being convenient, environmentally friendly, and non-toxic. UV-visible spectrophotometry, FT-IR, XRD, particle size analyser, SEM-EDAX and TEM methods were used to describe the physicochemical properties of Wt extract mediated synthesized CuNPs (Wt-CuNPs). The Wt-CuNPs synthesized was found to be monodispersed and spherical, with an average size of 15 nm. Gas chromatography and mass spectrometry (GC-MS) research revealed that the Wt R.Br plant extract contains various phytochemical compounds. The properties of Wt-CuNPs were verified by the findings of characterization tests. Via in silico molecular docking experiments with established targets, the underlying mechanisms of cytotoxicity against breast cancer and larvicidal behaviour against Aedes aegypti of Wt-CuNPs were investigated. Interestingly, in vitro cytotoxicity studies showed 50% cell death (IC50) of Wt-CuNPs treated MCF-7 cells and Vero Cells (Kidney epithelial cells) were displayed at 119.23 µg.mL-1 and 898.75 µg.mL-1, respectively. Also, Wt-CuNPs showed least LC50 and LC90 values for larvicidal activity against A. aegypti were of 32.10 µg.mL-1 and 21.70 µg.mL-1, respectively. Furthermore, Wt-CuNPs is found to be less toxic and biocompatible in haemolytic assays. The findings clearly showed that biosynthesized Wt-CuNPs have been used as a possible anticancer and larvicidal agent, as well as being environmentally friendly.
RESUMEN
BACKGROUND: Aedes aegypti is a primary vector of dengue virus, and the causative agent of dengue is emerging globally as one of the most important arboviral diseases currently threatening human populations. Therefore, vector control is presently the primary intervention method of population reduction, in which natural A. aegypti populations would be reduced with inhabitant bacterial strains that are unable to transmit dengue virus. RESULT: Based on the pathogenicity of strains, only four isolates effectively show larvicidal activity. The 16S rRNA gene sequences and the phylogeny depicted that the potential isolates were Bacillus firmus (MK791255), Bacillus paramycoides (MK788268), Bacillus siamensis (MK788212), and Bacillus licheniformis (MK791256). After 24 and 48 hours exposure, the B. licheniformis strain (cell mass of 2.2 × 107 CFU mL-1 ) showed potent larvicidal activity with LC50 of 16.22 µg mL-1 and 9.57 µg mL-1 and the B. paramycoides (cell mass of 3.1 × 107 CFU mL-1 ) strain inhibits the larval and pupal development with LC50 of 42.62 µg mL-1 and 26.97 µg mL-1 . Intermittent stages and causes of abscess in the gut and siphon regions were observed through histopathological studies. These two bacterial strains extend larval duration up to 15-16 days as well as reduce development. CONCLUSION: These studies demonstrate the challenge for dengue vector in reducing developmental and reproduction competence. © 2020 Society of Chemical Industry.