RESUMEN
DNA samples obtained from the populations of the Red Data Book species Bellevalia sarmatica (Georgi) Worovow from Volgograd oblast were examined using RAPD and AFLP analyses. DNA marking revealed considerable differences in the levels of interpopulation and intraspecific polymorphism of the Bellevalia. Furthermore, RAPD analysis, despite of lower levels of interpopulation polymorphism identified, made it possible to obtain clearer data on population subdivision upon statistical treatment of the results. The results of this study can be used in developing conservation strategies for maintenance of the species abundance and genetic diversity, as well as in elaborating the criteria for construction genetic collections.
Asunto(s)
Liliaceae/genética , Filogeografía , Hojas de la Planta/genética , Polimorfismo Genético , Análisis del Polimorfismo de Longitud de Fragmentos Amplificados/métodos , Genoma de Planta , Técnica del ADN Polimorfo Amplificado Aleatorio/métodos , Federación de RusiaRESUMEN
We have analyzed genetic effects of heterologous plant genes insertion on genome functioning of higher plants, belonging to different systematic groups (tobacco, Arabidopsis). Plants of different species were responding differently to the insertion of the same transgene, which is likely to be associated with the location of alien DNA insertion and could manifest in morphological changes spectrum and target gene expression level.
Asunto(s)
Arabidopsis/genética , Vectores Genéticos , Mutagénesis Insercional , Nicotiana/genética , Transgenes , Mutagénesis Insercional/métodosRESUMEN
The possibility of using RAPD and AFLP methods for genetic monitoring of populations of Matthiola fragrans (Bunge), a species included in the Red Book of the USSR, was shown for the first time. An analysis of inter- and intrapopulation and interspecies genome polymorphism was performed. Differences in the genetic structure of Matthiola populations from various geographical collection points were revealed. A simple method of performing RAPD analysis and the great number of unique markers found in each population compared with the AFLP analysis, as well as the good division of populations under statistical treatment, allow us to draw the conclusion that using the RAPD method in genetic monitoring of rare and insufficiently studied species is well founded.
Asunto(s)
Análisis del Polimorfismo de Longitud de Fragmentos Amplificados , Brassicaceae/genética , Monitoreo del Ambiente/métodos , Técnica del ADN Polimorfo Amplificado Aleatorio , Extinción Biológica , Variación Genética , Polimorfismo Genético , Federación de RusiaRESUMEN
The stability of marker nptII and target gene of serine proteinase inhibitor BWI-1a (ISP) from buckwheat seeds after agrobacterial transformation in the new foreign genome environment has been investigated. Different vegetative and seed progeny of self-pollinated transgenic tobacco was studied after propagation at selective and nonselective in vitro conditions. For the majority of transgene lines among random sample of independent transgenic plants stable and coupled PCR confirmed inheritance and expression of marker and target genes has been demonstrated after prolonged microcutting and repeated regeneration cycles at unselective in vitro conditions. The most of the seed T1 progeny of the studied lines has maintained Km-resistance too. However all transgenic lines had significant disturbances at the microsporogenesis process. Two lines (C5 and C12) after 4-year microcutting lacked Km-resistance and at the same time lacked their antibacterial activity. Two other clones revealed variability in Km-resistance (line C7) and seedlings size (line C22) that might be a result of more than 1 transgene insertion or chimaeric nature of transgene plants so it require an additional study.
Asunto(s)
Nicotiana/genética , Proteínas de Plantas/genética , Semillas/genética , Caulimovirus/genética , Resistencia a Medicamentos , Marcadores Genéticos , Kanamicina/farmacología , Plantas Modificadas Genéticamente , Regiones Promotoras Genéticas , Semillas/citología , Semillas/efectos de los fármacos , Nicotiana/citología , Nicotiana/efectos de los fármacos , TransgenesRESUMEN
The notion of 'psychic trauma" is defined. Medical criteria and characteristic clinical manifestations are discussed that may be used for expert estimation of the degree of health hazard in the form of psychogenic psychic disorder. It is shown that they are applicable to the evaluation of the consequences of a psychic trauma as exemplified by the results of forensic psychiatric expert examination of sexual offence victims carried out at V.P. Serbsky Research Centre of Social and Forensic Psychiatry by clinical and psychopathological methods.
Asunto(s)
Víctimas de Crimen/psicología , Testimonio de Experto , Psiquiatría Forense/legislación & jurisprudencia , Trastornos Mentales/psicología , Delitos Sexuales/legislación & jurisprudencia , Estrés Psicológico/psicología , Adaptación Psicológica , Abuso Sexual Infantil/legislación & jurisprudencia , Abuso Sexual Infantil/psicología , Preescolar , Víctimas de Crimen/legislación & jurisprudencia , Femenino , Humanos , Trastornos Mentales/diagnóstico , Federación de Rusia , Índice de Severidad de la Enfermedad , Estrés Psicológico/diagnósticoRESUMEN
Based on the analysis of 51 cases of sexual crimes committed against persons with mean age 11,1 years, main points of expert analysis of statement or exclusion of cause-effect relationship between mental disorder of a victim and psychogenic action of sexual infringement in the legal expertise of "health harm severity" are presented. The regularities of development of mental disorder in judicially significant situations with regard to the influence of different types of psychogenic factors (specific sexual traumatization, additional and concomitant factors, non-specific psychogenic factors) are described. Definitions of different variants of cause-effect relationships (directed, modified) are formulated. The author suggests the grounds for choosing a form (definite, probable) of expert conclusion.
Asunto(s)
Adaptación Psicológica/fisiología , Abuso Sexual Infantil/psicología , Víctimas de Crimen/psicología , Psiquiatría Forense/métodos , Trastornos Mentales/etiología , Estrés Psicológico/complicaciones , Niño , Abuso Sexual Infantil/legislación & jurisprudencia , Conducta Infantil/psicología , Víctimas de Crimen/legislación & jurisprudencia , Femenino , Estudios de Seguimiento , Humanos , Masculino , Trastornos Mentales/diagnóstico , Trastornos Mentales/psicología , Índice de Severidad de la Enfermedad , Estrés Psicológico/diagnóstico , Estrés Psicológico/psicologíaRESUMEN
The authors propose and discuss approaches to the expert evaluation of the severity of harm to the health in the form of mental disorder associated with the traumatic impact. The following qualifying signs of the degree of harm to the health included in the current legislation are considered in terms of forensic psychiatry: "life-threatening harm" (Criminal Code of the Russian Federation, article 111) and "length/brevity of impairment of the health" (Criminal Code of the Russian Federation, articles 112, 115). The well-grounded proposals of changes in the existing legislation envisage that the qualifying sign "permanent maladjustment" should be introduced into the corresponding legal innovations of the Criminal Code as useful for the forensic medical examination of victims with mental disorders. Substantive definition of such notions as "transient disadaptation", "permanent maladjustment" and degree of its manifestation (mild, moderate, severe) are given.