RESUMEN
The effect of toxins of a Cyanobacterium sample of the Shershnevo Reservoir on DNA, which was presented by cyanobacteria of the Microcystis genera, on the bone marrow of male CBA mice (whose age was 3 months and weight 24 g) was evaluated. With intraperitoneal administration, LD50 and LD16 of this sample for male CBA mice were 48.4 and 42.1 mg/kg, respectively. Administration of Microcystis cyanobacterial sample from the Shershnevo Reservoir in doses of 1/10 of LD16 and 1/2 of LD16, and LD16 was found to cause a dose-dependent reduction in the number of bone marrow nucleated cells, a dose-dependent increase in the rate of cell apoptotic death, a reduction in the duration of a cell cycle (within the first 12 hours), which gave way to an increase in the duration of the cycle 24 hours after administration, a dose-dependent increase in the frequency of micronuclei in the murine bone marrow eryphrocytes, and a dose-dependent decrease in the polychromatophil/normochromatophil ratio in the murine bone marrow.
Asunto(s)
Apoptosis/efectos de los fármacos , Toxinas Bacterianas/toxicidad , Células de la Médula Ósea/efectos de los fármacos , Ciclo Celular/efectos de los fármacos , ADN/efectos de los fármacos , Enfermedades Ambientales/genética , Toxinas Marinas/toxicidad , Microcistinas/toxicidad , Microcystis/aislamiento & purificación , Animales , Células de la Médula Ósea/patología , Toxinas de Cianobacterias , Modelos Animales de Enfermedad , Enfermedades Ambientales/microbiología , Enfermedades Ambientales/patología , Masculino , Ratones , Ratones Endogámicos DBA , Neurotoxinas , Federación de Rusia , Contaminantes del Agua/análisisRESUMEN
The toxic properties of cyanobacteriae of the Shershnevo water storage basin have been studied. Over almost the whole vegetative period of 2005, the cyanobacteriae of the Shershnevo water storage basin may be characterized as high toxic (when intraperitoneally injected to male CBA mice, LD50 was less than 100 mg/kg). There were no interspecific differences between the noninbred animals in cyanotoxin susceptibility. Gender differences were revealed in cyanotoxin susceptibility in CBA and noninbred albino mice: quantitative differences in LD50 (males/females) were 1.34 in CBA mice and 1.3 times in noninbred albino mice. The cyanobacteriae of the Shershnevo water storage basin were found to have an irritating effect on the skin and ocular mucosa. It is suggested that the toxins of Shershnevo water storage basin cyanobacteriae may have a damaging effect on human beings.