RESUMEN
Analysis of a region of chromosomal DNA lying between jadR1 and jadI in the gene cluster for jadomycin biosynthesis in Streptomyces venezuelae ISP5230 detected an ORF encoding 584 amino acids similar in sequence to the biotin carboxylase (BC) and biotin carboxyl carrier protein (BCCP) components of acyl-coenzyme A carboxylases. Multiple sequence alignments of the deduced Jad protein with acyl-coenzyme A carboxylases from various sources located the BC and BCCP components in the N- and C-terminal regions, respectively, of the deduced polypeptides. The organization and amino acid sequence of the deduced polypeptide most closely resembled those in other Gram-positive bacteria broadly classified as actinomycetes. Disrupting the gene, designated jadJ, severely reduced but did not eliminate jadomycin production. The disruption had no effect on growth or morphology of the organism, implying that the product of jadJ is not essential for fatty acid biosynthesis. It is concluded that jadJ supplies malonyl-coenzyme A for biosynthesis of the polyketide intermediate that is eventually processed to form the antibiotic jadomycin B.
Asunto(s)
Ligasas de Carbono-Carbono/genética , Isoquinolinas/metabolismo , Streptomyces/genética , Streptomyces/metabolismo , Secuencia de Aminoácidos , Ligasas de Carbono-Carbono/metabolismo , Regulación Bacteriana de la Expresión Génica , Datos de Secuencia Molecular , Alineación de SecuenciaRESUMEN
Over a period of 39 months, ending March, 1964, 1000 unselected cases were autopsied - 787 cases of neonatal death and 213 of still births. In the neonatal series 39 percent were premature males, 35 percent premature females, 17 percent full-term males, 29 percent full term females, 21 percent premature males, and 20 percent premature females. In the neonatal period 67 percent of the deaths occurred within the first seven days (AU)