RESUMEN
Biodegradation is the economically viable solution to restore land contaminated by hazardous pollutants such as benzo(a)pyrene (BaP). The present study focuses on the biodegradation of benzo(a)pyrene by Sphingobacterium spiritovorum in contaminated soil. The biodegradation kinetics and bacterial growth were evaluated while the biokinetic model that described the benzo(a)pyrene biodegradation was established. The Monod, Haldane, Powell and Edward models were used to model the bacterial growth in benzo(a)pyrene contaminated soil. Excel template was developed with Fourth order Runga-Kutta numerical algorithm to find the biokinetic parameters of the complex non-linear regression model. An Excel Solver function was used to obtain reasonable best-fit values of kinetic parameters. The Haldane and Edward models are well fit to describe the growth trend and model the kinetics of benzo(a)pyrene biodegradation. Enzyme substrate inhibition is the critical factor that affects the benzo(a)pyrene degradation by S. spiritovorum, which the model defines physically. The results demonstrated that removing benzo(a)pyrene showed positive interaction between substrate inhibition, the concentration of benzo(a)pyrene and sorption of the contaminants on soil particles.
RESUMEN
Pre-exposure of Echerichia coli to sub-lethal concentrations of cupric sulphate induced copper tolerance: pre-exposed (habituated) organisms were essentially unaffected by concentrations of Cu2+ which completely prevented colony formation by non-habituated ones. The observed copper tolerance was not dependent on the selection of copper-resistant mutants but resulted from a phenotypic change in the organisms during the pre-exposure period.