RESUMEN
The indirect tube LAI (leukocyte adherence inhibition) test has revealed that peripheral blood lymphocytes of rats of the inbred Lewis strain immunized with liver B77 tumor cells incubated in the presence of a specific tumor extract, release a factor into the culture medium that inhibits adherence of normal syngeneic peritoneal cells. Supernatants in which lymphocytes of healthy - control animals had been incubated in the presence of the same extract (B77), or lymphocytes of immunized donors incubated in the presence of another type of tumor extract (MC-1) were devoid of such a property. This soluble factor was found in the supernatants only after 20 hours of incubation of sensibilized lymphocytes with a specific tumor extract. Th percentage of adhering cells decreased in dependence on the concentration of the soluble factor. The results are discussed in connection with questions relevant to the mechanism of LAI reactivity and the application of this phenomenon in studies on cell immunity.
Asunto(s)
Inmunidad Celular , Inmunización , Técnicas Inmunológicas , Prueba de Inhibición de Adhesión Leucocitaria , Neoplasias Experimentales/inmunología , Animales , Femenino , Linfocitos/inmunología , Linfocinas/fisiología , RatasRESUMEN
A modified form of the leukocyte adherence inhibition (LAI) test has been used to follow cell-mediated immunity (CMI) in rats of the Lewis inbred strain (LW), F1 hybrids inbred strains LW x AVN with transplanted syngeneic tumors B77, MC-1, and the random-bred Sprague Dawley strain with spontaneous REF tumor. Peripheral blood leukocytes of animals incubated witha specific tumor extract showed an adherence capacity by 30--40% lower than the same cell population cultured without this specific extract, or with a foreign nonspecific extract. The results are commented in connection with a possible application of this modified form of LAI test in studies of the immunological state of patients with a malignant disorder.