RESUMEN
<p><b>OBJECTIVE</b>To investigate the effect of saponins from Tribulus terrestris (STT) on the renal carcinoma cell (786-0) in vitro, and inhibitory mechanisms.</p><p><b>METHOD</b>Effects of SIT on the cytotoxicity, morphological changes of apoptosis, cell cycle and expression of Bcl-2 protein in the 786-0 were tested respectively by MTT method, Wright and acridine orange stain assay, as well as flow cytometry (FCM).</p><p><b>RESULT</b>After the 786-0 was treated by STY, it was shown that: 1) A significant cytotoxic effect was observed by MTT assay; 2) Apoptosis-induced was viewed by Wright and acridine orange stain assay; 3) The distribution of 786-0 on S phase was increased; 4.) The expression of Bcl-2 protein and cyclin D1 was decreased.</p><p><b>CONCLUSION</b>STT can significantly inhibit the growth of 786-0 in vitro, partially, by apoptosis.</p>
Asunto(s)
Humanos , Antineoplásicos Fitogénicos , Farmacología , Apoptosis , Carcinoma de Células Renales , Metabolismo , Patología , Línea Celular Tumoral , Proliferación Celular , Ciclina D1 , Metabolismo , Medicamentos Herbarios Chinos , Farmacología , Neoplasias Renales , Metabolismo , Patología , Plantas Medicinales , Química , Proteínas Proto-Oncogénicas c-bcl-2 , Metabolismo , Fase S , Saponinas , Farmacología , Tribulus , QuímicaRESUMEN
<p><b>OBJECTIVE</b>To investigate the inhibitory and apoptosis-inducing effects of saponins from Tribulus terrestris (STT) on liver cancer cell line BEL-7402.</p><p><b>METHOD</b>MTT, SRB, Wright staining, acridine orange staining, flow cytometry, and Immunofluorescence microscopy were used to evaluate the effects of STT on BEL-7402 cell line.</p><p><b>RESULT</b>SMT had potent inhibitory effect on BEL-7402 cell line in a concentration-dependent manner. BEL-7402 cells exibited typical morphological alteration of apoptosis when sub-G1 peak could be seen. The expression of Bcl-2 was decreased in STT treated cells as compared with untreated control cells.</p><p><b>CONCLUSION</b>STT exerts its cytotoxic effect on BEL-7402 cells by inducing apoptosis.</p>