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Due to the scarcity of wild fruiting bodies, submerged fermentation of the medicinal fungus Antrodia camphorata is attracting much attention, but the production of bioactive triterpenoids is low. Therefore, there is an urgent need to improve the triterpenoid yield of submerged fermentation. Here, the A. camphorata mutant E3-64 was generated from strain AC16101 through random mutagenesis breeding, producing 172.8 mg triterpenoid per gram of dry mycelia. Further optimization of culture parameters resulted in a yield of 255.5 mg/g dry mycelia (i.e., an additional >1.4-fold increase), which is the highest reported yield thus far. Notably, mutant E3-64 produced 94% and 178% more of the triterpenoid components antcin A and antcamphin A, respectively, while it produced 52% and 15% less antcin B and G, respectively. Mutant E3-64 showed increased expression of key genes involved in triterpenoid biosynthesis, as well as different genome-wide single-nucleotide polymorphisms as compared with AC16101. Triterpenoids of the E3-64 mycelia exhibited remarkably protective activity against acute CCl4-induced liver injury in mice. This study shows the potential of A. camphorata for scientific research and commercial application.

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The phospholipid fatty acid (PLFA) technique was used to investigate the effects of fertilization on soil characteristics and microbial community of tomato in a solar greenhouse in Shouguang, Shandong Province, China, based on a long-term (12-year) fertilization experiment. The experiment involved a control (CK) and five fertilization treatments, namely, traditional nitrogen application (CN), traditional nitrogen application+straw return (CNS), optimized nitrogen application (SN), optimized nitrogen application+straw return (SNS), and organic manure nitrogen application+straw return (MNS). Results showed that the contents of soil organic matter and avai-lable P and K under all fertilization treatments were significantly higher than that of CK, but no significant difference among fertilization treatments. The traditional nitrogen application (i.e., CN and CNS) significantly decreased soil pH. The reduction of nitrogen fertilizer (i.e., SN, SNS and MNS) did not affect soil pH. Compared with CK, no straw treatment (i.e., CN and SN) and MNS did not affect the content of soil available N, but nitrogen combined with straw returned (i.e., CNS and SNS) significantly increased soil available N content. The optimized N with straw return (i.e., SNS) treatment resulted in the highest soil available N. Compared with CK and treatments without straw (i.e., CN, SN), treatments with straw (i.e., MNS, CNS and SNS) substantially changed soil microbial community structure and increased the biomass of soil bacteria, fungi, actinomycetes, mycorrhizal fungi, and the total amount of PLFA. SNS treatment had the highest soil microbial diversity, the highest biomass of soil bacteria, fungi, and actinomycetes, the highest total amount of PLFA, the highest ratios of fungi/bacteria and monounsaturated fatty acids/saturated fatty acids (MONO/SAT), the two indicators for soil ecosystem stability. Further, it had the lowest ratios of iso/anteiso fatty acids (i/a) and gram-positive/gram-negative bacteria (G+/G-), the indicators for nutritional stress. Redundancy and correlation analysis revealed that soil organic matter was the main factor affecting soil microbial community structure, with a significant positive correlation with actinomycetes and gram-positive bacteria. In conclusion, straw returning combined with optimized nitrogen application (i.e., SNS, 8 t·hm-2 of wheat straw with N fertilizer reduced by 58.3%) could improve soil nutrient status, soil microbial biomass, soil microbial community structure, and soil ecological environment. It would be an effective measure for reducing fertilizer application and improving efficiency.

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RESUMEN

OBJECTIVE: To evaluate the clinical efficacy and safety of angong niuhuang pill (ANP) as an adjuvant treatment on moderate or severe neonatal hypoxic-ischemic encephalopathy (NHIE). METHODS: Thirty-nine neonates with NHIE in the control group were treated with conventional treatment, and 58 in the treated group were administered orally ANP additionally, and relative indexes were observed. RESULTS: The improvement of aspects such as recovery of consciousness, muscular tension, and primitive reflex and disappearance of convulsion, in the treated group was better than that in the control group (P < 0.01). CONCLUSION: ANP as an adjuvant treatment has a definite effect on NHIE, it can promote the recovery of patients, decrease the occurrence of sequelae and with high safety, therefore, is a drug feasible for clinical application.

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