RESUMEN
OBJECTIVE: The purpose of this study was to identify genes related to bladder cancer with samples from normal and disease cases by microarray chip. METHODS: After downloading the gene expression profile GSE3167 from Gene Expression Omnibus database which includes 50 bladder samples, comprising 9 normal and 41 disease samples, differentially expressed genes were identified with packages in R language. The selected differentially expressed genes were further analyzed using bioinformatics methods. Firstly, molecular functions, biological processes and cell component analysis were researched by software Gestalt. Then, software String was used to search interaction relationships among differentially expressed genes, and hub genes of the network were selected. Finally, by using plugins of software Cytoscape, Mcode and Bingo, module analysis of hub-genes was performed. RESULTS: A total of 221 genes were identified as differentially expressed by comparing normal and disease bladder samples, and a network as well as the hub gene C1QBP was obtained from the network. The C1QBP module had the closest relationship to production of molecular mediators involved in inflammatory responses. CONCLUSION: We obtained differentially expressed genes of bladder cancer by microarray, and both PRDX2 and YWHAZ in the module with hub gene C1QBP were most significantly related to production of molecular mediators involved in inflammatory responses. From knowledge of inflammatory responses and cancer, our results showed that, the hub gene and its module could induce inflammation in bladder cancer. These related genes are candidate bio-markers for bladder cancer diagnosis and might be helpful in designing novel therapies.
Asunto(s)
Biomarcadores de Tumor/genética , Perfilación de la Expresión Génica , Regulación Neoplásica de la Expresión Génica , Análisis de Secuencia por Matrices de Oligonucleótidos , Neoplasias de la Vejiga Urinaria/genética , Vejiga Urinaria/metabolismo , Algoritmos , Estudios de Casos y Controles , Humanos , Pronóstico , ARN Mensajero/genética , Reacción en Cadena en Tiempo Real de la Polimerasa , Reacción en Cadena de la Polimerasa de Transcriptasa InversaRESUMEN
In this paper, the noise performances of 1.5 microm correlated photon pair generation based on spontaneous four wave-mixing in three types of fibers, i.e., dispersion shifted fiber, traditional highly nonlinear fiber and highly nonlinear microstructure fiber are investigated experimentally. Result of the comparison shows that highly nonlinear microstructure fiber has the lowest Raman noise photon generation rate among the three types of fibers while correlated photon pair generation rate is the same. Theoretical analysis indicates that the noise performance is determined by the nonlinear index and Raman response of the material in fiber core. The Raman response rises with increasing doping level, while, for the nonlinear index, the impact of doping level is weak. As a result, highly nonlinear microstructure fiber with pure silica core has the best noise performance and great potential in practical sources of correlated photon pairs and heralded single photons.
Asunto(s)
Simulación por Computador , Tecnología de Fibra Óptica/instrumentación , Fibras Ópticas , Fotones , Dispersión de Radiación , Espectrometría Raman/métodos , Diseño de EquipoRESUMEN
OBJECTIVE: To study how blood supply of the esophageal and gastric stumps influences the anastomotic healing after esophagogastrostomy in rabbits. METHODS: Twenty-seven New Zealand rabbits were randomly divided into 3 groups to receive esophagogastrostomy, followed by different procedures. Except for those in group I, all the rabbits were subjected to procedures of reducing the blood supply either of the esophageal or the gastric stump (group II and group III, respectively), followed by single-layer esophagogastric anastomoses using interrupted 5-0 polypropylene sutures. Ten days after operation, all the rats were killed and the anastomotic sites excised for measurement of the inner diameter, tensile strength, and hydroxyproline concentration. RESULTS: Healing of the esophagograstric anastomosis was obtained in all the rabbits but one with anastomotic leakage in group I and one with perforation of the gastric fundus in group III. The anastomotic inner diameters were similar in all the three groups, whereas the tensile strength and hydroxyproline concentration at the anastomoses decreased in group III in comparison with the other two groups (P<0.05) that had similar measurements (P>0.05). CONCLUSIONS: Extended length of the free esophageal stump does not significantly affect anastomotic healing as decrease of blood supply in the gastric stump.