RESUMEN
OBJECTIVE: To develop an HPLC method for the content determination of benzoic acid, 4-hydroxyphenylacetic acid and acetosyringonecas in Ficus microcarpa Leaves. METHODS: The determination was performed on Purospher® STAR C18 (250 mm x 4.6 mm,5 µm). The mobile phase was consisted of acetonitrile-0.2% phosphoric acid aqueous solution with linear gradient elution and the flow rate of 1.0 mL/min. The column temperature was set at 35 °C and the detection wavelength was 270 nm. RESULTS: The linear range of benzoic acid, 4-hydroxyphenylacetic acid and acetosyringonecas was 0.0121-1.21 µg (r = 0.9995), 0.423-42.32 [Lg ( r = 0.9999) and 0.047-4.70 pg( r = 0. 9996) , respectively. The average recovery was 100.7, 101.2 and 96.5 respectively. CONCLUSION: This method is simple,reproducible,and can be used for determination of three components of benzoic acid, 4-hydroxyphenylacetic acid and acetosyringonecas in Ficus microcarpa Leaves.
Asunto(s)
Cromatografía Líquida de Alta Presión , Ficus/química , Fitoquímicos/química , Hojas de la Planta/química , Plantas Medicinales/química , Fenilacetatos , Fitoquímicos/aislamiento & purificaciónRESUMEN
OBJECTIVE: To study the relevance between the drug effects and serum HPLC fingerprint of chemical constituents in blood from Ficus microcarpa leaves. METHODS: Fingerprints of the preparation and drug-containing serum of rats were established by HPLC. Based on the fingerprints, chemical constituents in blood were investigated. RESULTS: There were seven common peaks in the HPLC fingerprints of drug-containing serum of rats. Polarity fraction of the chemical constituents in blood existed in the ethyl acetate extract, and five fingerprint peaks of serum HPLC fingerprint existed in n-butanol extract. CONCLUSION: Determination of the component groups in blood provides some data on material basis study in vivo for Ficus microcarpa leaves.
Asunto(s)
Ficus/química , Hojas de la Planta/química , Animales , Cromatografía Líquida de Alta Presión , RatasRESUMEN
OBJECTIVE: To compare the volatile constituents of Plumeriae Flos from different origins. METHODS: Water distillation method according to the Chinese Pharmacopeia was used to extract the volatile constituents of fresh Plumeriae Flos samples (red flower and white flower) and dried samples. GC-MS method combined with NIST MS Search 2.0 data base was carried out to identify the volatile constituents and to calculate the relative percentage content. RESULTS: 55 peaks were detected in the GC-MS spectrum. Among of them, 26 volatile constituents were confirmed and calculated, which were mainly fatty alcohols and esters. The relative percentage content of geranyl benzoate in fresh Plumeriae Flos samples was significantly higher than the dried samples. CONCLUSION: The compositions of volatile constituents in Plumeriae Flos have no obvious correlation with the color of flowers, but are related to the flower fresh or dried.