RESUMEN

Vehicle re-identification holds great significance for intelligent transportation and public safety. Extracting vehicle recognition information from multi-view vehicle images has become one of the challenging problems in the field of vehicle recognition. Most recent methods employ a single network extraction structure, either a single global or local measure. However, for vehicle images with high intra-class variance and low inter-class variance, exploring globally invariant features and discriminative local details is necessary. In this paper, we propose a Feature Fusion Network (GLFNet) that combines global and local information. It utilizes global features to enhance the differences between vehicles and employs local features to compactly represent vehicles of the same type. This enables the model to learn features with a large inter-class distance and small intra-class distance, significantly improving the model's generalization ability. Experiments show that the proposed method is competitive with other advanced algorithms on three mainstream road traffic surveillance vehicle re-identification benchmark datasets.

RESUMEN

Marker-based prediction holds great promise for improving current plant and animal breeding efficiencies. However, the predictabilities of complex traits are always severely affected by negative factors, including distant relatedness, environmental discrepancies, unknown population structures, and indeterminate numbers of predictive variables. In this study, we utilised two independent F1 hybrid populations in the years 2012 and 2015 to predict rice thousand grain weight (TGW) using parental untargeted metabolite profiles with a partial least squares regression method. A stable predictive model for TGW was built based on hybrids from the population in 2012 (r = 0.75) but failed to properly predict TGW for hybrids from the population in 2015 (r = 0.27). After integrating hybrids from both populations into the training set, the TGW of hybrids could be predicted but was largely dependent on population structures. Then, core hybrids from each population were determined by principal component analysis and the TGW of hybrids in both environments were successfully predicted (r > 0.60). Moreover, adjusting the population structures and numbers of predictive analytes increased TGW predictability for hybrids in 2015 (r = 0.72). Our study demonstrates that the TGW of F1 hybrids across environments can be accurately predicted based on parental untargeted metabolite profiles with a core hybridisation strategy in rice. Metabolic biomarkers identified from early developmental stage tissues, which are grown under experimental conditions, may represent a workable approach towards the robust prediction of major agronomic traits for climate-adaptive varieties.

Asunto(s)

Grano Comestible/crecimiento & desarrollo , Metaboloma , Oryza/crecimiento & desarrollo , Biomarcadores , Grano Comestible/metabolismo , Ambiente , Hibridación Genética , Análisis de los Mínimos Cuadrados , Oryza/metabolismo , Fitomejoramiento

RESUMEN

As knowledge of the structure and function of nucleic acid molecules has increased, sequence-specific DNA detection has gained increased importance. DNA biosensors based on nucleic acid hybridization have been actively developed because of their specificity, speed, portability, and low cost. Recently, there has been considerable interest in using nano-materials for DNA biosensors. Because of their high surface-to-volume ratios and excellent biological compatibilities, nano-materials could be used to increase the amount of DNA immobilization; moreover, DNA bound to nano-materials can maintain its biological activity. Alternatively, signal amplification by labeling a targeted analyte with nano-materials has also been reported for DNA biosensors in many papers. This review summarizes the applications of various nano-materials for DNA biosensors during past five years. We found that nano-materials of small sizes were advantageous as substrates for DNA attachment or as labels for signal amplification; and use of two or more types of nano-materials in the biosensors could improve their overall quality and to overcome the deficiencies of the individual nano-components. Most current DNA biosensors require the use of polymerase chain reaction (PCR) in their protocols. However, further development of nano-materials with smaller size and/or with improved biological and chemical properties would substantially enhance the accuracy, selectivity and sensitivity of DNA biosensors. Thus, DNA biosensors without PCR amplification may become a reality in the foreseeable future.