RESUMEN
Aquaculture wastewater contains high concentrations of nitrogen and phosphorus compounds, which can be used as nutrients for microalgal growth. In this study, the ability of Chlorella sorokiniana (C. sorokiniana) to purify aquaculture wastewater from an intensive recirculating aquaculture system (RAS) carp sp. farm was evaluated. We then assessed the safety risk of C. sorokiniana cultured from aquaculture wastewater and conducted an 8-week fish feeding trial to evaluate its nutritional value as a feed protein source. The three diets were supplemented with 0 (FM, control), 5% (MM5) or 15% (MM15) C. sorokiniana to replace the fish meal. A total of 180 healthy gibel carp (Carassius gibelio) of similar size were randomly selected into 9 tanks (20 fish/tank, 3 tanks/group). At the end of C. sorokiniana purifying aquaculture wastewater, DIC and DTC gradually decreased by 80.6% and 16.5%, respectively, whereas DOC increased by 52.2%. The change curve of CODMn was similar to that of DOC, and the removal rates of NH4-N, DTN, DIP and DTP were 93.5%, 86.8%, 36.0% and 26.6%, respectively. The heavy metals and antibiotics contents of C. sorokiniana were low or not detected and conformed to the requirements of the aquatic feed ingredient standards. The ARA, EPA and total polyunsaturated fatty acids contents of C. sorokiniana were 13.67, 33.82 and 76.81% of the total fatty acids content, respectively. At the end of the fish feeding trial, we found that the replacement of fishmeal with C. sorokiniana did not affect the growth of the fish or the amino acids contents of the muscle but promoted the body colour values of the fish and the relative content of n-3 polyunsaturated fatty acids in the muscle. In addition, 5% dietary C. sorokiniana can upregulate the relative expression of cat and increase the activity of CAT in the liver; upregulate the relative expression of the proinflammatory factor inf-γ and the anti-inflammatory factors il-4 and tgf-ß; and reduce the relative abundance of pathogenic bacteria, such as Citrobacter, Staphylococcus and Pseudomonas, in the gut of gibel carp. However, 15% dietary C. sorokiniana significantly increased the relative expression of inf-γ and hsp70 in the liver and only reduced the relative abundance of Citrobacter. Overall, C. sorokiniana has the ability to remove nutrients from aquaculture wastewater and can be an alternative protein source for fish. On the basis of growth performance, antioxidant capacity, fatty acid contents of muscle, and the gut microbiota, 5% dietary C. sorokiniana is recommended.
RESUMEN
An 88-day feeding trial was conducted to evaluate the effects of dietary inosine 5'-monophosphate (5'-IMP) on the growth performance and salinity and oxidative stress resistance in the juvenile gibel carp CAS III (Carassius auratus gibelio; initial body weight: 7.48 g). Four isonitrogenous and isoenergetic diets containing exogenous 5'-IMP were formulated. P1, P2, P3 and P4 were diets containing 5'-IMP at four concentrations (0, 1, 2 and 4 g kg-1). The four diets were randomly allotted to triplicate tanks in a recirculating system. After the feeding trial, six fish per tank were netted randomly and placed into 12‱ saline water to test their response to salinity stress. The results indicated that the feed conversion rate was enhanced by dietary supplementation with 5'-IMP. The appetite, plasma neuropeptide Y level and feeding rate of the P3 group were lower than those in the control treatment group. Dietary supplementation with 5'-IMP improved the osmoregulatory adaptation of gibel carp under acute salinity stress. Six hours after the salinity stress treatment, in the dietary 5'-IMP treatment group, the plasma cortisol and K+ concentrations were lower and the Na+/K+-ATPase activity was greater than that in the control group. Dietary supplementation with 5'-IMP promoted the expression of the glucocorticoid receptors NKA-α1b and NKCC and retarded the expression of Hsp70 in P4-treated gill filaments and kidneys. Dietary supplementation with 5'-IMP resulted in a stable oxidative-stress-resistant phenotype characterized by increased levels of cellular antioxidants, including SOD, catalase, glutathione peroxidase, glutathione reductase and MPO. The above results of the current study demonstrate that supplementation of 5'-IMP can promote feed utilization and have positive influences on the salinity and oxidative stress resistance of gibel carp.
RESUMEN
The active ingredients extracted from yeast are important for regulating animal health. The aim of the current research was to explore the impacts of dietary yeast glycoprotein (YG) on the growth performance, intestinal morphology, antioxidant capacity, immunity and disease resistance of largemouth bass (Micropterus salmoides). A total of 375 juvenile fish (6.00 ± 0.03 g) were allocated into 15 fiberglass tanks. Triplicate tanks were assigned to each diet. The dietary YG inclusion was as follows: the first group was given a high fishmeal diet (40% fishmeal, 0% YG) (FM) and the second group was given a low fishmeal diet (30% fishmeal and 15% soybean meal, 0% YG) (LFM). The fish in the third, fourth and fifth groups were fed the LFM diet supplemented with 0.5% (LFM+YG0.5), 1.0% (LFM+YG1.0) and 2.0% (LFM+YG2.0) YG, respectively. After a 60- day feeding trial, a challenge test using A. hydrophila was carried out. The results showed that the final body weight (FBW) and weight gain rate (WGR) in the LFM+YG2.0 group were significantly higher than those in the LFM group and were no significantly different from those in the FM group. This may be partially related to the activation of the target of rapamycin (TOR) signaling pathway. Dietary YG supplementation enhanced intestinal physical barriers by upregulating the intestinal tight junction protein related genes (claudin1, occludin and zo2) and improving the structural integrity of the gut, which may be partially associated with AMPK signaling pathway. Moreover, dietary YG increased the antioxidant capacity in the gut, upregulated intestinal anti-inflammatory factors (il-10, il1-1ß and tgf-ß) and downregulated proinflammatory factors (il-1ß and il-8), which may be partially related to the Nrf2/Keap1 signaling pathways. The results of the challenge test indicated that dietary supplementation with 0.5 or 1.0% YG can increase the disease tolerance of largemouth bass against A. hydrophila. In conclusion, the present results indicated that dietary supplementation with YG promotes the growth performance, intestinal immunity, physical barriers and antioxidant capacity of largemouth bass. In addition, 1.0% of dietary YG is recommended for largemouth bass based on the present results.