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KEY MESSAGE: We developed the ZDX1 high-throughput functional soybean array for high accuracy evaluation and selection of both parents and progeny, which can greatly accelerate soybean breeding. Microarray technology facilitates rapid, accurate, and economical genotyping. Here, using resequencing data from 2214 representative soybean accessions, we developed the high-throughput functional array ZDX1, containing 158,959 SNPs, covering 90.92% of soybean genes and sites related to important traits. By application of the array, a total of 817 accessions were genotyped, including three subpopulations of candidate parental lines, parental lines and their progeny from practical breeding. The fixed SNPs were identified in progeny, indicating artificial selection during the breeding process. By identifying functional sites of target traits, novel soybean cyst nematode-resistant progeny and maturity-related novel sources were identified by allele combinations, demonstrating that functional sites provide an efficient method for the rapid screening of desirable traits or gene sources. Notably, we found that the breeding index (BI) was a good indicator for progeny selection. Superior progeny were derived from the combination of distantly related parents, with at least one parent having a higher BI. Furthermore, new combinations based on good performance were proposed for further breeding after excluding redundant and closely related parents. Genomic best linear unbiased prediction (GBLUP) analysis was the best analysis method and achieved the highest accuracy in predicting four traits when comparing SNPs in genic regions rather than whole genomic or intergenic SNPs. The prediction accuracy was improved by 32.1% by using progeny to expand the training population. Collectively, a versatile assay demonstrated that the functional ZDX1 array provided efficient information for the design and optimization of a breeding pipeline for accelerated soybean breeding.

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BACKGROUND: Pregabalin has received wide clinical attention as a new type of analgesic. We undertake a systematic review and meta-analysis to evaluate the effect of pregabalin on postoperative pain in patients undergoing cardiac surgery. METHODS: We searched PubMed, Embase, and Cochrane Library (from inception to July 2020) for eligible studies. The primary outcomes were the total morphine consumption at 24 h. A secondary outcome was intraoperative fentanyl consumption, extubation time postoperative, and length of stay in hospital. We calculated pooled weighted mean difference (WMD) or odds ratio (OR) and 95% CIs using random- or fixed-effects models. RESULTS: Seven trials involving 463 patients were listed. Meta-analysis showed that the total morphine consumption at 24 h in the pregabalin group was significantly less than the control group (WMD: -5.44, 95% CI: -10.42-0.46, P = 0.03). We found that there is no significant difference between the two groups in intraoperative fentanyl consumption. Compared with the control group, the length of stay in hospital in the pregabalin group was significantly shorter (WMD = -0.87, 95% CI: -1.42-0.32, P = 0.002). And we found that there were no significant differences between the two groups in extubation time (WMD: 17.24, 95% CI: -24.36-58.84, P = 0.42). CONCLUSIONS: Oral pregabalin for cardiac surgery patients can effectively reduce the patient's 24-hour morphine consumption after surgery, shorten the patient's hospital stay, and is more conducive to early postoperative recovery.

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Hydrogen is an ideal energy carrier due to its abundant reserves and high energy density. Electrolyzing water is one of the carbon free technologies for hydrogen production, which is limited by the sluggish kinetics of the half reaction of the anode - the oxygen evolution reaction (OER). In this study, a self-supported Cu3P nanowire (Cu3P NWs/CF) electrode is prepared by electrodeposition of a Cu(OH)2 nanowire precursor on conductive Cu foam (Cu(OH)2 NWs/CF) with a subsequent phosphating procedure under a N2 atmosphere. When used as an OER working electrode in 1.0 M KOH solution at room temperature, Cu3P NWs/CF exhibits excellent catalytic performance with an overpotential of 327 mV that delivers a current density of 20 mA cm-2. Notably, it can run stably for 22 h at a current density of 20 mA cm-2 without obvious performance degradation. This highly efficient and stable OER catalytic performance is mainly attributed to the unique nanostructure and stable electrode construction. Interestingly, this synthesis strategy has been proved to be feasible to prepare large-area working electrodes (e.g. 40 cm-2) with unique nanowire structure. Therefore, this work has provided a good paradigm for the mass fabrication of self-supporting non-noble metal OER catalysts and effectively promoted the reaction kinetics of the anode of the electrolyzing water reaction.