RESUMEN
BACKGROUND: The Five Welfare Needs in UK animal welfare legislation underpin a legal duty of care and are an animal welfare assessment framework. Health and welfare problems arise when these needs are unmet. The veterinary professions work with others to address these problems, but there is no publicly funded U.K. companion animal welfare surveillance to identify priorities, or promote and monitor change. METHODS: The veterinary charity, the People's Dispensary for Sick Animals (PDSA), together with the market research organisation, YouGov, has undertaken a longitudinal nationwide survey, assessing whether the U.K's pet dogs, cats and rabbits are having their Five Welfare Needs met. Data from nationally representative samples of pet-owning adults, drawn from YouGov's online survey panel, have been used to produce the PDSA Animal Wellbeing (PAW) Report annually since 2011. RESULTS: Examples are given of how the PAW Report has been used to monitor trends in animal welfare problems, drive collaborative behaviour change campaigns, create evidence-based funding applications and inspire innovation in veterinary practice. CONCLUSION: The PAW Report has contributed to closing a gap in national companion animal welfare surveillance. When governments rely on non-governmental organisations to assist with animal welfare surveillance, reliable sources such as the PAW Report can inform research, policy and legislation.
Asunto(s)
Bienestar del Animal/normas , Práctica Clínica Basada en la Evidencia/organización & administración , Mascotas , Medicina Veterinaria , Adulto , Bienestar del Animal/legislación & jurisprudencia , Animales , Gatos , Perros , Humanos , Masculino , Mascotas/psicología , Conejos , Encuestas y Cuestionarios , Reino UnidoRESUMEN
The Lnk adapter protein negatively regulates the signaling of thrombopoietin (TPO), the main megakaryocyte (MK) growth factor. Lnk-deficient (-/-) mice have increased TPO signaling and increased MK number. Interestingly, several mouse models exist in which increased MK number leads to a high bone mass phenotype. Here we report the bone phenotype of these mice. MicroCT and static histomorphometric analyses at 20 weeks showed the distal femur of Lnk-/- mice to have significantly higher bone volume fraction and trabecular number compared to wild-type (WT) mice. Notably, despite a significant increase in the number of osteoclasts (OC), and decreased bone formation rate in Lnk-/- mice compared to WT mice, Lnk-/- mice demonstrated a 2.5-fold greater BV/TV suggesting impaired OC function in vivo. Additionally, Lnk-/- mouse femurs exhibited non-significant increases in mid-shaft cross-sectional area, yet increased periosteal BFR compared to WT femurs was observed. Lnk-/- femurs also had non-significant increases in polar moment of inertia and decreased cortical bone area and thickness, resulting in reduced bone stiffness, modulus, and strength compared to WT femurs. Of note, Lnk is expressed by OC lineage cells and when Lnk-/- OC progenitors are cultured in the presence of TPO, significantly more OC are observed than in WT cultures. Lnk is also expressed in osteoblast (OB) cells and in vitro reduced alkaline phosphatase activity was observed in Lnk-/- cultures. These data suggest that both direct effects on OB and OC as well as indirect effects of MK in regulating OB contributes to the observed high bone mass. J. Cell. Biochem. 118: 2231-2240, 2017. © 2017 Wiley Periodicals, Inc.
Asunto(s)
Péptidos y Proteínas de Señalización Intracelular/metabolismo , Osteoclastos/citología , Osteoclastos/metabolismo , Trombopoyetina/metabolismo , Proteínas Adaptadoras Transductoras de Señales , Fosfatasa Alcalina/genética , Fosfatasa Alcalina/metabolismo , Animales , Apoptosis/genética , Apoptosis/fisiología , Western Blotting , Células de la Médula Ósea/metabolismo , Ciclo Celular/genética , Ciclo Celular/fisiología , Diferenciación Celular/genética , Diferenciación Celular/fisiología , Proliferación Celular/genética , Proliferación Celular/fisiología , Femenino , Inmunoprecipitación , Péptidos y Proteínas de Señalización Intracelular/deficiencia , Péptidos y Proteínas de Señalización Intracelular/genética , Masculino , Megacariocitos/metabolismo , Proteínas de la Membrana , Ratones , Ratones Endogámicos C57BL , Osteoblastos/citología , Osteoblastos/metabolismo , Osteogénesis/genética , Osteogénesis/fisiología , Células RAW 264.7 , Trombopoyetina/genética , Microtomografía por Rayos XAsunto(s)
Composición Corporal/fisiología , Enfermedades de los Gatos/diagnóstico , Enfermedades de los Perros/diagnóstico , Sobrepeso/veterinaria , Tejido Adiposo/metabolismo , Animales , Enfermedades de los Gatos/epidemiología , Gatos , Enfermedades de los Perros/epidemiología , Perros , Sobrepeso/diagnóstico , Sobrepeso/epidemiologíaRESUMEN
BACKGROUND: Little is known about the frequency with which information from the Internet is presented by patients within primary care consultations or the subsequent impact that it may have on those consultations. OBJECTIVES: The aims of this study were to describe the frequency with which Internet information was presented within primary care consultations in one inner-city health authority and to describe the characteristics of the subsequent consultation from the perspective of the health professionals involved. METHODS: A postal survey was used to estimate the frequency of Internet information presentation and eight in-depth interviews were used to obtain health professionals' perceptions of the consultations that followed. RESULTS: Presentation of information from the Internet was relatively infrequent within primary care at the time of the survey (November 2000 to March 2001), but frequencies of presentation were higher for GPs than for any other health professional group. Health professionals have stereotypical views of Internet users and fear for their own professional status in relation to the Internet-informed patient or client. CONCLUSIONS: Although presentation of information from the Internet to date remains relatively infrequent, health professionals appear to feel threatened by it and adopt strategies that minimize its impact on the subsequent consultation.