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Cancer is one of the diseases with the highest mortality rate today, with breast cancer being the second most common type among the Brazilian population. Due to its etiological complexity and inefficiency of treatments, studies have focused on new forms of treatment. Among these forms of treatment, hormonal therapy seems to be an excellent auxiliary mechanism in tumoricidal activity, and melatonin has great potential as a modulator of the immune system. Thus, the present study is aimed at evaluating the effect of the hormone melatonin on the coculture of colostrum polymorphonuclear cells and MCF-7 cancer cells and evaluates the effect of this hormone using a modified transport system. A feasibility analysis was performed by fluorescence microscopy at three cell incubation times, 2 hours, 24 hours, and 72 hours. The measurement of cytokines in the cell supernatant occurred in 24 hours, and the apoptosis assay was performed in 72 hours using flow cytometry. The results showed higher levels of cell viability in groups treated with melatonin and less viability in groups containing a coculture of polymorphonuclear cells and MCF-7 after 72 hours of incubation. Furthermore, the apoptosis and necrosis rates were higher in coculture polymorphonuclear and MCF-7 cells, especially in groups containing microemulsion as a modified release agent. These data suggest that melatonin, especially if associated with a modified release system, has immunomodulatory effects on human colostrum polymorphonuclear cells. These cells can play a crucial role in the resolution of the tumor through their mediation and inflammatory action.
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The therapeutic administration of cytokines has been introduced aiming to modulate the immune response system, seeking for different approaches to face pathologies such as cancer, auto immune and infectious diseases. The objective of this study was to investigate the effects of a stable oil-in-water (O/W) nanoemulsion system carrying the cytokine Interferon gamma (IFN-γ) on the activity of phagocytes and MCF-7 human breast cancer cells. Nanoemulsions were prepared through ultra-homogenization, and they consisted of distilled water, triglycerides of capric acid/caprylic, sorbitan-oleate, polysorbate 80, and 1-butanol. IFN-γ (100 ng ml-1 ) was incorporated into two O/W nanoemulsion formulations, and these formulations were characterized in terms of their preliminary and accelerated physicochemical stability, rheological properties, droplet size, polydispersity and surface charge. We identified the most optimal IFN-γ nanoemulsion (IFN-γNE2), which remained stable under extreme temperature variations for 90 days, contained an average dose of 97 ng ml-1 of IFN-γ and exhibited a biocompatible pH and a relative stable rheological profile. Cell viability and intracellular Ca2+ release assays conducted showed that IFN-γNE2 reduced the cell viability of MCF-7 cells without affecting the cell viability of phagocytes. Furthermore, IFN-γNE2 was able to induce cellular activity of phagocytes as evidenced by increased intracellular Ca2+ release in these cells. Our findings on this IFN-γ nanoemulsion suggest that it can be a promising therapeutic agent for immunostimulation and cancer treatment.
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Antineoplásicos/administración & dosificación , Portadores de Fármacos/química , Emulsiones/química , Factores Inmunológicos/administración & dosificación , Interferón gamma/administración & dosificación , Adulto , Antineoplásicos/farmacología , Células Cultivadas , Femenino , Humanos , Factores Inmunológicos/farmacología , Interferón gamma/farmacología , Células MCF-7 , Masculino , Neoplasias/tratamiento farmacológico , Adulto JovenRESUMEN
AIM: We hypothesized that IL-1ß concentrations are augmented in overweight adolescents, who do not display metabolic syndrome. Additionally, we aimed to correlate the IL-1ß concentrations with several established risk factors for CVD. METHODS: Overweight or control subjects, aging from 14-18 years, were classified according to their adjusted body mass index and evaluated for biochemical and anthropometric parameters. The proinflammatory cytokine IL-1ß was assessed in the serum. RESULTS: Increased body fat percentage, waist circumference, triglycerides, total cholesterol, Very Low-Density Lipoprotein (VLDL) cholesterol, Low-Density Lipoprotein (LDL) cholesterol, Castelli I index, IL-1ß, and IL-8 levels, were observed in overweight adolescents. No differences were observed in systolic blood pressure, diastolic blood pressure, glucose or High-Density Lipoprotein (HDL) cholesterol. Positive correlations between IL-1ß with anthropometric and or biochemical parameters were found. CONCLUSION: In conclusion, increased IL-1ß levels correlate to dyslipidemic factors and may further support low-grade inflammation. IL-1ß may further predict the early onset of cardiovascular disease in this population, taking into consideration its important regulatory role.
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Factores de Riesgo de Enfermedad Cardiaca , Inflamación/sangre , Interleucina-1beta/sangre , Sobrepeso/sangre , Adolescente , Brasil/epidemiología , Enfermedades Cardiovasculares/sangre , Enfermedades Cardiovasculares/diagnóstico , Enfermedades Cardiovasculares/epidemiología , Enfermedades Cardiovasculares/etiología , Estudios de Casos y Controles , Enfermedad Crónica , Femenino , Humanos , Inflamación/epidemiología , Inflamación/patología , Masculino , Sobrepeso/complicaciones , Sobrepeso/epidemiología , Obesidad Infantil/sangre , Obesidad Infantil/complicaciones , Obesidad Infantil/epidemiología , Pronóstico , Factores de RiesgoRESUMEN
The resurgence of cases of Zika virus (ZIKV) infection, accompanied by epidemic of microcephaly in Brazil, has aroused worldwide interest in understanding the biological mechanisms of the virus that allow patient management and the viral dissemination control. Colostrum and human milk are possible sources of virus spread. Therefore, the objective of this study was to analyze the repercussions of ZIKV infection on rheological parameters and inflammatory cytokines of colostrum. The prospective cohort study included 40 puerperal donors of colostrum, divided into 2 groups: control (without ZIKV infection, n = 20) and a group infected with ZIKV during the gestational period (n = 20). Analyses were performed for the detection of ZIKV by polymerase chain reaction (PCR). In addition to obtaining the rheological parameters and quantification of IL-10 and IL-6 cytokines by flow cytometry, ZIKV and other flaviviruses were not detected in colostrum. However, maternal infection reflected increased viscosity, decreased levels of IL-10, and elevated levels of IL-6. The higher viscosity may represent a mechanical barrier that hinders the spread of the virus. The lower levels of anti-inflammatory mediators and higher inflammatory cytokines may possibly alter the viscosity, and it seems the higher viscosity represents a possible mechanism of adaptation of breastfeeding against a response to ZIKV.
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Calostro/inmunología , Interacciones Huésped-Patógeno/inmunología , Interleucina-10/inmunología , Interleucina-6/inmunología , Complicaciones Infecciosas del Embarazo/inmunología , Infección por el Virus Zika/inmunología , Virus Zika/patogenicidad , Adulto , Estudios de Casos y Controles , Calostro/química , Femenino , Expresión Génica , Humanos , Interleucina-10/genética , Interleucina-6/genética , Periodo Posparto/inmunología , Embarazo , Complicaciones Infecciosas del Embarazo/genética , Complicaciones Infecciosas del Embarazo/virología , Reología , Viscosidad , Virus Zika/inmunología , Infección por el Virus Zika/genética , Infección por el Virus Zika/virologíaRESUMEN
BACKGROUND AND OBJECTIVES: Breastfeeding promotion is an important public health strategy for counter-balancing the negative effects of maternal overweight and obesity. Colostrum contains melatonin, which can attenuate the impacts of excessive maternal weight and boost the infant's immune system. Therefore, the objective of this study was to analyze the effects of melatonin on mononuclear (MN) phagocytes from the colostrum of women with pre-gestational obesity. Materials and Methods: Colostrum samples were collected postpartum from 100 women at a public hospital in São Paulo, Brazil. The donors were divided into two groups: the control group and the high body mass index (BMI) group. Melatonin levels in the colostrum were determined by an ELISA Kit, and the functional activity of MN cells was assessed using the phagocytosis assay by flow cytometry, and reactive oxygen species (ROS), intracellular calcium, and apoptosis were assessed by fluorimetry using a microplate reader. RESULTS: The colostrum of mothers with pre-gestational high BMI exhibited higher melatonin levels (p < 0.05) and lower phagocytosis (p < 0.05) and ROS release (p < 0.05). Superoxide release was similar between the normal and high BMI groups (p > 0.05). Intracellular calcium release and apoptosis were also higher in the high BMI group (p < 0.05). Melatonin levels likely increased the phagocytosis rate and reduced intracellular calcium release and the apoptosis index (p < 0.05). CONCLUSIONS: The results suggest that melatonin is a possible mechanism for maternal-infant protection against obesity and restores the functional activity of colostrum phagocytes in obese mothers.
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Calostro/inmunología , Melatonina/fisiología , Obesidad/fisiopatología , Fagocitos/fisiología , Fagocitosis/fisiología , Adulto , Índice de Masa Corporal , Lactancia Materna , Calostro/química , Femenino , Humanos , Melatonina/análisis , Melatonina/farmacología , Fagocitos/efectos de los fármacos , Especies Reactivas de Oxígeno/metabolismoRESUMEN
Adiponectin and leptin play roles in the hunger response, and they can induce the inflammatory process as the initial mechanism of the innate immune response. It is possible for alterations in the levels of these adipokines to compromise the functional activity of human colostrum phagocytes. Therefore, the objective of this study is to analyze the effects of adiponectin and leptin on colostrum mononuclear (MN) cells. Colostrum was collected from 80 healthy donors, who were divided into two groups: the control group and the high body mass index (BMI) group. MN cells were used to analyze phagocytosis by flow cytometry, and reactive oxygen species (ROS), intracellular calcium, and apoptosis were assessed by fluorimetry using a microplate reader. Adipokines restored the levels of phagocytosis to the high BMI group (p < 0.05), with a mechanism that is action-dependent on the release of ROS and intracellular calcium. However, adiponectin and leptin simultaneously contributed to better microbicidal activity, thus reflecting an increase in the apoptosis level (p < 0.05) in the high BMI group. Probably, the maintenance of the balance between adiponectin and leptin levels enhances the protection and decreases the indices of neonatal infection in the breastfeeding infants of women with high BMI values. Therefore, policies that support pre-gestational weight control should be encouraged.
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Adiponectina/farmacología , Inflamación/patología , Leptina/farmacología , Leche Humana/metabolismo , Obesidad/patología , Adulto , Apoptosis/efectos de los fármacos , Índice de Masa Corporal , Calcio/metabolismo , Calostro/efectos de los fármacos , Femenino , Humanos , Fagocitosis/efectos de los fármacos , Embarazo , Estallido Respiratorio/efectos de los fármacosRESUMEN
Purpose: Changes in regulatory T cells (Treg) in peripheral blood are associated with a number of pathologies, including diabetes. However, the immunological responses of pregnant diabetic women remain scarcely known, and the effects of Treg cells in these patients have yet to be investigated. The present study characterized the expression of regulatory T cells in the maternal blood, cord blood and placenta of diabetic pregnant women. Materials and methods: The women were divided according to glycemic status into a non-diabetic (ND; N = 20) or type 2 diabetic (T2DM; N = 20) group. Cell subsets were determined by flow cytometry. Results: Compared to ND, T2DM blood cells exhibited a higher expression of CD25+, Foxp3+, CD4+CD25+, CD4+Foxp3+ and CD25+Foxp3+; and cord blood cells showed a lower expression of CD25+, CD4+Foxp3+ and CD25+Foxp3+. In the placenta of T2DM, the villous layer of the proportion, CD3+ and CD25 was lower than that of CD4+Foxp3+ and CD25+Foxp3+, and the extravillous placenta layer contained the lowest levels of CD4+ and CD25+ and highest proportions of CD4+Foxp3+. In maternal blood from T2DM, the frequency of CD3+CD95+ and CD3CD4+ T cells expressing CD95+ was lower. In cord blood from T2DM, the rate of CD3+CD95+ was lower. The placenta villous layer of T2DM showed a lower count of CD3+CD95+ and of CD3CD4+ T cells expressing CD95+, whereas the number of cells expressing CD3+CD45RO+ decreased in both placental layers. Conclusion: The data obtained suggest that hyperglycemia changes the phenotypes of regulatory T cells and Fas expression in memory T cells.
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Diabetes Mellitus Tipo 2/inmunología , Diabetes Gestacional/inmunología , Linfocitos T Reguladores/citología , Adulto , Estudios de Casos y Controles , Femenino , Sangre Fetal/citología , Sangre Fetal/inmunología , Humanos , Placenta/inmunología , Embarazo , Adulto JovenRESUMEN
AIMS: The interleukin-10 (IL-10) is an immuno-regulatory cytokine that plays a protective effect in the vasculature. IL-10 binding to its receptor, activating the IL-10/JAK1/STAT3 cascade to exert its effects. Therefore, STAT3 phosphorylation is essential for IL-10 actions. O-Glycosylation with linked ß-N-acetylglucosamine (O-GlcNAc) is a post-translational modification able to regulate many proteins by interfering with protein on a phosphorylation level. Our aim was to determine whether O-GlcNAc promotes the inhibition of IL-10-pathway (JAK1/STAT3/IL-10), inactivationg its action in the vasculature. MAIN METHODS: Mice (C57BL/6) aortic segments were incubated with vehicle or Thiamet G (0.1â¯mM, for 24â¯h) to increase global O-GlcNAc levels. Aortas from knockout mice for IL-10 were also used. Vascular reactivity and western blot tests were performed to evaluate protein expression. KEY FINDINGS: High levels of O-GlcNAc, induced by Thiamet G incubation, increased vascular expression of JAK1, but decreased expression and activity of STAT3. In addition, IL-10 levels were diminished in arteries treated with Thiamet G. Absence of IL-10, as well as augmented O-GlcNAcylation, increased vascular reactivity to constrictor stimuli, an effect that was abolished by ERK 1/2 inhibitor. High levels of O-GlcNAc and the absence of IL-10 also leads to increased vascular expression of ERK1/2. SIGNIFICANCE: Our data suggest that O-GlcNAc modification seems to (dys)regulate IL-10 signaling pathway and consequently, compromise the protective effect of this cytokine in vasculature. It is possible that there is a promising relationship in pathophysiological conditions where changes in O-GlcNAcylation and IL-10 levels are observed, such as hypertension and diabetes.
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Acetilglucosamina/química , Interleucina-10/química , Interleucina-10/metabolismo , Procesamiento Proteico-Postraduccional , Vasoconstricción , Animales , Glicosilación , Transducción de SeñalRESUMEN
BACKGROUND AND OBJECTIVE: The adipose tissue has been recognized as an important endocrine organ, which is metabolically active and expresses and secretes various inflammatory cytokines. Inflammation is involved in obesity-related complications. As such, the present study investigated the correlation between biochemical parameters, serum proinflammatory cytokines and adipokines in individuals with obesity. METHODS: Based on the body mass index (BMI), 30 subjects were divided into 3 groups: eutrophic (GC, n = 10), overweight (GOW, n = 10) and obese (GOB, n = 10). Serum glucose, cholesterol (total-C, HDLC and LDL-C), triglycerides, total proteins, uric acid and insulin were determined, as well as cytokines IL-8, TNF-α, IL-1ß, and IL-6, leptin and adiponectin. RESULTS: GOB showed the highest glucose, total and LDL-C, triglycerides, uric acid, insulin, leptin, IL- 8, IL-1ß, IL-6, TNF-α and lowest adiponectin levels. In general, adiponectin exhibited an inverse correlation with BMI, abdominal circumference, LDL-C, IL-6, TNF-α, leptin and leptin-adiponectin ratio (LAR) and a positive correlation with HDL-C. Leptin was positively correlated with BMI, abdominal circumference, insulin, IL-6, TNF-α and LAR and negatively correlated with HDL-C and adiponectin. The LAR was positively correlated with BMI, waist circumference, insulin, TNF-α and negatively associated with HDL-C. CONCLUSION: The results confirm that obesity changes the lipid and glycemic profiles of individuals, increases the proinflammatory adipokine levels and reduces those of anti-inflammatory adipokines, promoting a state of chronic inflammation.
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Adipoquinas/sangre , Quimiocinas/sangre , Citocinas/sangre , Obesidad/sangre , Obesidad/diagnóstico , Adulto , Biomarcadores/sangre , Femenino , Humanos , Mediadores de Inflamación/sangre , Masculino , Persona de Mediana Edad , Circunferencia de la Cintura/fisiología , Adulto JovenRESUMEN
The study investigated the role of cytokines IL-4 and IL-17 in the modulation of the functional activity of mononuclear phagocytes in diabetic pregnant women with hyperglycemia. Sixty pregnant women were assigned to the following groups: nondiabetic (ND), mild gestational hyperglycemia (MGH), gestational diabetes mellitus (GDM), or type 2 diabetes mellitus (DM2). The functional activity of phagocytes from maternal blood, cord blood, and colostrum was assessed by determining their superoxide release, phagocytosis, microbicidal activity, and intracellular Ca2+ release. Irrespective of glycemic status, colostrum and blood cells treated with IL-4 and IL-17 increased superoxide release in the presence of enteropathogenic Escherichia coli (EPEC). The highest phagocytosis rate was observed in cells from the DM2 group treated with IL-4. In all the groups, phagocytes from colostrum, maternal blood, and cord blood exhibited higher microbicidal activity against EPEC when treated with cytokines. IL-17 increased intracellular Ca2+ release by colostrum phagocytes in diabetic groups. The results indicate that the IL-4 and IL-17 modulate the functional activity of phagocytes in the maternal blood, cord blood, and colostrum of diabetic mother. The natural immunity resulting from the interaction between the cells and cytokines tested may be an alternative procedure to improve the prognosis of maternal and newborn infections.
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Diabetes Gestacional/inmunología , Interleucina-17/fisiología , Interleucina-4/fisiología , Fagocitos/inmunología , Adolescente , Adulto , Femenino , Humanos , Persona de Mediana Edad , Fagocitosis , Embarazo , Adulto JovenRESUMEN
OBJECTIVES: To investigate the chronopharmacological effects of growth hormone on executive function and the oxidative stress response in rats. MATERIALS AND METHODS: Fifty male Wistar rats (36-40 weeks old) had ad libitum access to water and food and were separated into four groups: diurnal control, nocturnal control, diurnal GH-treated, and nocturnal GH-treated animals. Levels of Cu, Zn superoxide dismutase (Cu, Zn-SOD), and superoxide release by spleen macrophages were evaluated. For memory testing, adaptation and walking in an open field platform was used. GH-treated animals demonstrated better performance in exploratory and spatial open-field tests. RESULTS: The latency time in both GH-treated groups was significantly lower compared with the latency time of the control groups. The diurnal GH treatment did not stimulate superoxide release but increased the CuZn-SOD enzyme levels. The nocturnal GH treatment did not influence the superoxide release and CuZn-SOD concentration. GH treatment also resulted in heart atrophy and lung hypertrophy. CONCLUSION: Growth hormone treatment improved the performance of executive functions at the cost of oxidative stress triggering, and this effect was dependent on the circadian period of hormone administration. However, GH treatment caused damaging effects such as lung hypertrophy and heart atrophy.
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BACKGROUND: Obesity in pregnancy is associated with systemic inflammation, immunological changes and adverse maternal-fetal outcomes. Information on the association between maternal obesity and breast milk composition is scarce. This study describes changes and relationships between biochemical and immunological parameters of colostrum and serum of overweight and obese women. METHODS: Colostrum and blood samples were collected from 25 normal weight, 24 overweight and 19 obese women for determination of glucose, total protein, triglycerides, cholesterol, immunoglobulins, complement proteins (C3 and C4), fat and calorie content and C-reactive protein (CRP). RESULTS: Glucose was higher in colostrum of obese women (p = .002). In normal weight and obese women, total protein content was higher in colostrum than in serum (p = .001). Serum triglycerides (p = .008) and cholesterol (p = .010) concentrations were significantly higher in overweight and obese women than in their normal weight counterparts, but in colostrum their concentrations were similar across the three groups. Secretory IgA (sIgA) in colostrum and IgA in serum concentrations were significantly higher (p = .001) in overweight and obese mothers, whereas IgG and IgM concentrations did not vary among the groups (p = .825). Serum C3 (p = .001) and C4 (p = .040) concentrations were higher in obese women. No differences in colostrum complement proteins were detected among the groups. Calorie content (p = .003) and fat (p = .005) concentrations in colostrum and serum CRP (p = .002) were higher in obese women. CONCLUSIONS: The results corroborate the hypothesis that colostrum of overweight and obese women undergoes biochemical and immunological changes that affect its composition, namely increasing glucose concentrations, calorie content, fat and sIgA concentrations.
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Calostro/inmunología , Obesidad/inmunología , Complicaciones del Embarazo/inmunología , Adolescente , Adulto , Glucemia/metabolismo , Proteína C-Reactiva/inmunología , Estudios de Casos y Controles , Colesterol/metabolismo , Calostro/metabolismo , Complemento C3/inmunología , Complemento C4/inmunología , Estudios Transversales , Grasas/metabolismo , Femenino , Glucosa/metabolismo , Humanos , Inmunoglobulinas/inmunología , Obesidad/metabolismo , Sobrepeso/inmunología , Sobrepeso/metabolismo , Embarazo , Complicaciones del Embarazo/metabolismo , Proteínas/metabolismo , Triglicéridos/metabolismo , Adulto JovenRESUMEN
Melatonin is involved in a number of physiological and oxidative processes, including functional regulation in human milk. The present study investigated the mechanisms of action of melatonin and its effects on the functional activity of colostral phagocytes in diabetic women. Colostrum samples were collected from normoglycemic (Nâ=â38) and diabetic (Nâ=â38) women. We determined melatonin concentration, superoxide release, bactericidal activity and intracellular Ca(2+) release by colostral phagocytes treated or not with 8-(Diethylamino) octyl-3,4,5-trimethoxybenzoate hydrochloride (TMB-8) and incubated with melatonin and its precursor (N-acetyl-serotonin-NAS), antagonist (luzindole) and agonist (chloromelatonin-CMLT). Melatonin concentration was higher in colostrum samples from hyperglycemic than normoglycemic mothers. Melatonin stimulated superoxide release by colostral phagocytes from normoglycemic but not hyperglycemic women. NAS increased superoxide, irrespective of glycemic status, whereas CMTL increased superoxide only in cells from the normoglycemic group. Phagocytic activity in colostrum increased significantly in the presence of melatonin, NAS and CMLT, irrespective of glycemic status. The bactericidal activity of colostral phagocytes against enterophatogenic Escherichia coli (EPEC) increased in the presence of melatonin or NAS in the normoglycemic group, but not in the hyperglycemic group. Luzindole blocked melatonin action on colostrum phagocytes. Phagocytes from the normoglycemic group treated with melatonin exhibited an increase in intracellular Ca(2+) release. Phagocytes treated with TMB-8 (intracellular Ca(2+) inhibitor) decreased superoxide, bactericidal activity and intracellular Ca(2+) release in both groups. The results obtained suggest an interactive effect of glucose metabolism and melatonin on colostral phagocytes. In colostral phagocytes from normoglycemic mothers, melatonin likely increases the ability of colostrum to protect against EPEC and other infections. In diabetic mothers, because maternal hyperglycemia modifies the functional activity of colostrum phagocytes, melatonin effects are likely limited to anti-inflammatory processes, with low superoxide release and bactericidal activity.
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Antioxidantes/farmacología , Calostro/citología , Calostro/inmunología , Melatonina/farmacología , Fagocitos/efectos de los fármacos , Fagocitos/inmunología , Adolescente , Adulto , Calcio/metabolismo , Estudios Transversales , Escherichia coli/inmunología , Femenino , Humanos , Hiperglucemia/inmunología , Hiperglucemia/metabolismo , Persona de Mediana Edad , Fagocitos/metabolismo , Fagocitosis/efectos de los fármacos , Fagocitosis/inmunología , Embarazo , Superóxidos/metabolismo , Adulto JovenRESUMEN
Viral and non-viral hepatitis are of great concern among developing nations because of their pathogenicity and virulence, and also their wide spreading by contaminated blood, food or water. The objective of this work was to evaluate the knowledge about hepatitis of academic students from three life/health sciences courses and also students from the last year of high school To measure the students' knowledge on hepatitis an instrument containing 22 questions was applied. Surprinsingly, it was verified that 41.9% of students had poor knowledge of viral hepatitis. Among the high school students, 31.8% ignored that viral hepatitis are infectious and transmissible diseases. Considering hepatitis symptomatology, just 18% of high school students declared knowledge of the symptons, but none of those cited the ictericia. Among the academic students, 75.9% of nursing students had adequate knowledge of hepatitis, followed by pharmacy (51.3%), and biology students (18.2%). Nursing students had also higher scores of right answers regarding viral hepatitis and chronic disease. On contrary, biology and high school students had poor knowledge of that matter (37% and 44.5%, respectively). Less than 15% of nursing and pharmacy students did not know that viral hepatitis are sexually transmissible, whereas 78.6% of the 3rd year and 52.4% of the 4th year biology course ignored the sexual transmission of viral hepatitis. Still considering the same question, 54.5% of the high school students also ignored that viral hepatitis are sexually transmitted diseases. Important conclusions can be drawn from this study, since the higher hepatitis knowledge scores were found among nursing students, followed by pharmacy academics. However, biology students, which will serve as high school teachers, had poor and insufficient knowledge on hepatitis. This finding could explain the same poor disease knowledge among high school pupils.
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Conocimientos, Actitudes y Práctica en Salud , Hepatitis Viral Humana , Estudiantes/estadística & datos numéricos , Brasil , Femenino , Humanos , Masculino , Encuestas y Cuestionarios , Adulto JovenRESUMEN
Although oxygen, nitrogen, and chlorine reactive species have been associated with disease pathogenesis, their partial absence is very harmful to the body's innate immune defense. Lacking of adequate release of free radicals from activated phagocytes is related to impaired ability on fungi, bacteria, and protozoa killing. We constructed an updated conceptual landmark regarding the paramount role of free radicals in phagocyte defense systems (phagocyte oxidase, myeloperoxidase, and nitric oxide/peroxynitrite system) on natural immunity. Diverse fungal, bacterial and protozoal pathogens evade the phagocytes' oxidative/nitrosative burst though antioxidant genes, enzymes and proteins. The most important evasion mechanisms were also described and discussed. These interconnected systems were reviewed and discussed on the basis of knowledge from relevant research groups around the globe. Phagocyte-derived free radicals are essential to destroy important human pathogens during the course of innate immunity.
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Evasión Inmune , Inmunidad Innata/inmunología , Estrés Oxidativo/inmunología , Fagocitos , Especies Reactivas de Oxígeno , Antioxidantes/metabolismo , Candida albicans/inmunología , Candida albicans/metabolismo , Candida glabrata/inmunología , Candida glabrata/metabolismo , Humanos , Macrófagos/inmunología , Macrófagos/metabolismo , Malaria/inmunología , Malaria/metabolismo , Mycobacterium tuberculosis/inmunología , Mycobacterium tuberculosis/metabolismo , Óxido Nítrico/inmunología , Óxido Nítrico/metabolismo , Oxidorreductasas/inmunología , Oxidorreductasas/metabolismo , Peroxidasa/inmunología , Peroxidasa/metabolismo , Ácido Peroxinitroso/inmunología , Ácido Peroxinitroso/metabolismo , Fagocitos/inmunología , Fagocitos/metabolismo , Especies Reactivas de Oxígeno/inmunología , Especies Reactivas de Oxígeno/metabolismo , Estallido Respiratorio/inmunologíaAsunto(s)
Personal Militar , Enfermedades Profesionales/psicología , Policia , Trastornos del Sueño del Ritmo Circadiano/psicología , Estrés Fisiológico/fisiología , Estrés Psicológico/psicología , Adulto , Brasil/epidemiología , Femenino , Humanos , Masculino , Personal Militar/psicología , Enfermedades Profesionales/epidemiología , Trastornos del Sueño del Ritmo Circadiano/complicaciones , Estrés Psicológico/epidemiología , Adulto JovenRESUMEN
BACKGROUND: Melatonin has immunomodulatory effects but very little is known about its influence in protozoan infections, such as Entamoeba histolytica, which causes amoebiasis, a disease with significant morbidity and mortality. In this study, we evaluated the effects of exogenous melatonin interference in experimental amoebiasis and on interactions between human blood cells and E. histolytica trophozoites. METHODS: The effect of melatonin was investigated in models of experimental amoebiasis in hamsters and rats by evaluating the area of necrosis induced by E. histolytica. The activity of melatonin on the interactions between leukocytes and amoebae was determined by examining leukophagocytosis. For in vitro tests, polymorphonuclear and mononuclear human blood leucocytes were incubated with E. histolytica trophozoites. RESULTS: The areas of amoebic necrosis were significantly reduced in animals treated with melatonin. Melatonin treatment increased leukophagocytosis but was associated with a greater number of dead amoebae. CONCLUSIONS: These results suggest that melatonin may play a beneficial role in the control of amoebic lesions, raising the possibility that this drug may be used as an adjuvant in anti-amoebic therapy.
Asunto(s)
Entamebiasis/patología , Entamebiasis/parasitología , Factores Inmunológicos/administración & dosificación , Melatonina/administración & dosificación , Adolescente , Adulto , Animales , Células Cultivadas , Cricetinae , Modelos Animales de Enfermedad , Entamoeba histolytica , Histocitoquímica , Humanos , Leucocitos/inmunología , Leucocitos/parasitología , Hígado/patología , Masculino , Microscopía , Fagocitosis/efectos de los fármacos , Ratas , Ratas Wistar , Enfermedades de los Roedores/parasitología , Enfermedades de los Roedores/patología , Adulto JovenRESUMEN
JUSTIFICATIVA E OBJETIVOS: A obesidade está associada a diversas doenças, como a hipertensão arterial, diabetes mellitus tipo 2, doenças cardiovasculares e cerebrovasculares, além do aumento do risco de neoplasias. No Brasil, o consumo de moderadores de apetite é um dos maiores do mundo. Deste modo, o objetivo deste estudo foi conhecer a prevalência de consumo de medicamentos para perda de peso e a prevalência de sobrepeso em população de estudantes universitários. MÉTODO: Foram avaliados 487 alunos do Campus Universitário do Araguaia, UFMT. Foi realizada avaliação do índice de massa corpórea (IMC) e aplicação de questionário para avaliação dos medicamentos para perda de peso mais consumidos, tempo de consumo, ocorrência de efeitos adversos, acompanhamento médico e motivo para seu uso. RESULTADOS: Dos entrevistados, 9% utilizaram medicamentos com intuito de emagrecer, dentre estes 47,7% estavam com IMC normal e 88,6% eram mulheres. A prevalência de sobrepeso nos estudantes foi de 22,9% dentre os homens e 9,6% dentre as mulheres. Os fármacos mais utilizados foram a quitosana (13,1%), seguidos pela alcachofra (Cynara scolymus L.), chá verde (Camelia sinensis), femproporex e sibutramina, com 6,7% cada. A reação adversa mais citada foi boca seca (21,6%). Dentre os estudantes, 41% afirmaram ter obtido informações acerca do medicamento utilizado com um médico. Além do medicamento para emagrecer, 52,5% dos entrevistados afirmaram utilizar dieta de restrição alimentar, 30% fizeram dieta e exercícios físicos. CONCLUSÃO: A maioria das mulheres que utilizaram medicamentos para perda de peso não precisavam fazê-lo.
BACKGROUND AND OBJECTIVES: Obesity associated with many diseases, such as arterial hypertension, type 2 diabetes mellitus, cardiovascular and cerebrovascular diseases, beyond the increased risk of neoplastic disorders. In Brazil, the consumption of anorexigen drugs is one of the most high in the world. Then, the objective of this work was to know the prevalence of overweight and use of weight loss drugs in a population of university students. METHOD: 487 students from the "Instituto Universitário do Araguaia", Universidade Federal de Mato Grosso (UFMT) were analyzed. Beyond the body mass index (BMI), a questionnaire was used to evaluate the most consumed weight loss drugs, time of drug use, occurrence of adverse health effects, medical consulting, and declared motive for using those drugs. RESULTS: Among the interviewed, 9.0% used drugs for weight loss, and between those 47.7% presented normal body mass index (BMI), and 88.6% were female. The prevalence of overweight was 22.9% for male and 9.6% for female students. The most used weight loss drugs were chitosan (13.1%), followed by alcachofra (Cynara scolymus L.), green tea (Camelia sinensis), fenproporex and sibutramine, with 6.7% each drug. The most frequent related adverse health effect was dry mouth (21.6%). Among the students, 41.0% declared had obtained drug information use with a medical doctor. Beyond use of weight loss drugs, 52.5% of the students declared use food restriction diets, and 30.0% were involved in both food restriction diets and practice of physical exercises. CONCLUSION: The majority of women had used weight loss drugs must not use it because of their normal body weight.
Asunto(s)
Humanos , Masculino , Femenino , Adulto , Obesidad/tratamiento farmacológico , Estudiantes , Pérdida de PesoRESUMEN
OBJECTIVE: To assess attitudes to breastfeeding among children in primary education and to evaluate the influence of educational lectures on their knowledge. METHODS: This was a cross-sectional study of 503 students of both sexes from the fourth to the eighth grades of five different schools. The children were divided into two groups: control (n = 215) and intervention (n = 288) in order to evaluate the influence of educational lectures. A questionnaire containing 30 questions about different features of breastfeeding was used for data collection. The intervention took the form of a 30-minute lecture, given at the schools. RESULTS: The lecture increased the number of schoolchildren who answered that breastmilk is most beneficial for infants (p < 0.05) and that breastfeeding is the most practical way of feeding babies (p < 0.05). There was a reduction in the number who would give supplementary feeding during the first month of life (p < 0.05) and also in how many would give a baby a pacifier (p < 0.05). After the intervention less than half of the girls would chose to breastfeed for 1 year or more (39.1% in the control group vs. 43.2% in the intervention group); while the opposite was true of the males (54.7% in the control group vs. 51.7% in the intervention group). More of the boys (37.1%) were able to cite specific advantages for mothers from breastfeeding when compared to the females (19.9%). CONCLUSIONS: The results indicate that lectures on health education, presented at schools, have a beneficial effect on breastfeeding knowledge, awareness and attitudes.
Asunto(s)
Lactancia Materna/psicología , Educación en Salud/métodos , Conocimientos, Actitudes y Práctica en Salud , Estudiantes/psicología , Niño , Estudios Transversales , Femenino , Humanos , Masculino , Encuestas y CuestionariosRESUMEN
OBJETIVO: Avaliar a percepção de estudantes do ensino fundamental em relação ao aleitamento materno e a influência de palestra educativa sobre seus conhecimentos. MÉTODOS: Realizou-se um estudo transversal em cinco escolas, envolvendo 503 estudantes da quarta à oitava série, de ambos os sexos, divididos em dois grupos, controle (n = 215) e de intervenção (n = 288), para avaliar a influência de palestra educativa. Para a coleta de dados, utilizou-se um questionário contendo 30 questões sobre diferentes aspectos da amamentação. A intervenção consistiu em uma palestra com duração de 30 minutos realizada nas escolas. RESULTADOS: A palestra aumentou o número de alunos que responderam que o leite materno é o mais adequado para a criança (p < 0,05) e que o ato de amamentar é a forma mais prática de alimentação (p < 0,05). Houve redução na intenção de realizar a suplementação alimentar no primeiro mês de vida (p < 0,05) e na oferta de chupeta ao bebê (p < 0,05). Após a intervenção, menos da metade das meninas optaria por um tempo de aleitamento igual a 1 ano ou mais (39,1 por cento no grupo controle versus 43,2 por cento no de intervenção); verificou-se o oposto para o sexo masculino (54,7 por cento no controle versus 51,7 por cento no de intervenção). Os meninos souberam citar vantagens específicas da amamentação para as mães com maior freqüência (37,1 por cento), quando comparados ao sexo feminino (19,9 por cento). CONCLUSÃO: Os resultados indicam que palestras de educação em saúde, realizadas em escolas, exercem influência benéfica sobre o conhecimento, percepção e atitudes em relação ao aleitamento materno.
OBJECTIVE: To assess attitudes to breastfeeding among children in primary education and to evaluate the influence of educational lectures on their knowledge. METHODS: This was a cross-sectional study of 503 students of both sexes from the fourth to the eighth grades of five different schools. The children were divided into two groups: control (n = 215) and intervention (n = 288) in order to evaluate the influence of educational lectures. A questionnaire containing 30 questions about different features of breastfeeding was used for data collection. The intervention took the form of a 30-minute lecture, given at the schools. RESULTS: The lecture increased the number of schoolchildren who answered that breastmilk is most beneficial for infants (p < 0.05) and that breastfeeding is the most practical way of feeding babies (p < 0.05). There was a reduction in the number who would give supplementary feeding during the first month of life (p < 0.05) and also in how many would give a baby a pacifier (p < 0.05). After the intervention less than half of the girls would chose to breastfeed for 1 year or more (39.1 percent in the control group vs. 43.2 percent in the intervention group); while the opposite was true of the males (54.7 percent in the control group vs. 51.7 percent in the intervention group). More of the boys (37.1 percent) were able to cite specific advantages for mothers from breastfeeding when compared to the females (19.9 percent). CONCLUSION: The results indicate that lectures on health education, presented at schools, have a beneficial effect on breastfeeding knowledge, awareness and attitudes.