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Objective To investigate the neuroprotective effect of 2-methoxyestradiol (2ME2) on neurological functions and nerve cell apoptosis in rats with acute cerebral infarction.Methods Seventy-two adult male SD rats were randomly divided into sham-operated group,model group and treatment group (n=24).Middle cerebral artery infarction models in the later two groups were constructed by Longa method.Rats in treatment group were treated with 2ME2 (24 mg/kg) daily for 7 d,while rats in the sham-operated group and model group with physiological saline.Bederson standard scale was adopted to analyze the nerve function defect 7 d after operation.Caspase-3 protein expression in the rat brain tissues was analyzed by Western blotting and apoptosis index was analyzed by TUNEL.Results As compared with those in the model group (2.015±0.871),Bederson standard scale scores in the treatment group (1.235±0.849) were significantly lower (P＜0.05);as compared with the sham-operated group (0.286±0.160,7.8 ±6.9),the model group and treatment group had significantly increased Caspase-3 protein level and apoptosis index (P＜0.05),while Caspase-3 protein level and apoptosis index in treatment group (0.615±0.185,27.8±6.4) were significantlylower than those in the model group (1.109±0.224,34.9±5.9,P＜0.05).Conclusion 2ME2 can improve the neurological function,reduce the level of apoptosis in rat brain nerve cells and has a protective effect on nerve.
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Objective To investigate the effect of 2-methoxyestradiol (2ME2) on phosphorylation of Tau protein (p-Tau) and mortor function of rats after spinal cord injury (SCI).Methods A total of 72 adult male Sprague-Dawley rats were randomly divided into 3 groups:sham-operated group (n=24),SCI group (n=24) and SCI+2ME2 treated group (n=24); models of SCI were created by modified Allen's method; one d after SCI,rats in the SCI+2ME2 treated group were given intraperitoneal injection of 2ME2 (24 mg/kg) for seven d,and rats in the SCI group were given the same volume of normal saline.Basso,Beatti,Bresnahan (BBB) scale was performed to evaluate the hindlimb function one,three,seven,14,21 and 28 d after SCI; the expression changes ofp-Tau (Ser 262) seven d after SCI were observed by immunofluorescence and Western blotting.Results BBB scale scores in the SCI group and SCI+2ME2 group were significantly increased following the prolonging of injury times (1-28 d of injury,P＜0.05); on the 7th,14th,21st and 28th d of injury,the scores in the SCI+2ME2 group were significantly higher than those in the SCI group (P＜0.05).The p-Tau positive cells in the spinal cord and the p-Tau (Ser 262) expression in the SCI group and SCI+2ME2 group were significantly increased as compared with those in the sham-operated group (P＜0.05),and those in the SCI+2ME2 group (19.05± 1.34 and 0.283±0.094) were obviously lower as compared with those in the SCI group (10.36±1.28 and 0.607±0.105,P＜0.05).Conclusion 2ME2 offers protection in rats with SCI by reducing the level of tau phosphorylation.
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Objective To investigate the protective effects of low-frequency pulsed electromagnetic field (LFPMF) on neuron following cerebral ischemia-reperfusion injury in rats.Methods 24 adult male Sprague-Dawley rats were randomly divided into irradiation group (n=12) and control group (n=12). The global cerebral ischemia-reperfusion models of rats were produced by modified Pulsineli method. Rats in irradiation group were exposed to 20 mT intensity LFPMF of 10 Hz for 45 minutes per day for 4 days. The electroencephalogram (EEG) was recorded by powerlab system. Nissl staining was used to perform quantitative analysis of neuron number and immunohistochemistry staining provided the information of the distribution of positive cells to caspase-3.Results Compared with the control group, the amplitude of EEG was significantly higher in irradiation group [(10.27?1.12)?V versus (8.95?1.04)?V] (P