RESUMEN

On the basis of monitoring the prevention of accumulation of lipid droplets in mouse 3T3-L1 preadipocyte cells and inhibition of the proliferation of human colon cancer HT-29 cells, effective anti-corpulence and anticancer compounds were isolated from the peel of Citrus fruits. These bioactive components were identified as polymethoxyflavones and coumarin derivatives by spectroscopic analyses. 5-Hydroxy-6,7,8,3',4'-pentamethoxyflavone had the greatest anti-corpulence effects and 3,5,6,7,8,3',5'-heptamethoxyflavone had the greatest anticancer effects. Furthermore, distributions of those bioactive components in the peel of 10 species of Citrus fruits were demonstrated by HPLC analyses.

Asunto(s)

Fármacos Antiobesidad/aislamiento & purificación , Antineoplásicos/aislamiento & purificación , Citrus/química , Cumarinas/aislamiento & purificación , Flavonas/aislamiento & purificación , Células 3T3-L1 , Adipocitos/efectos de los fármacos , Animales , Fármacos Antiobesidad/farmacología , Antineoplásicos/farmacología , Línea Celular Tumoral , Cumarinas/farmacología , Evaluación Preclínica de Medicamentos , Flavonas/farmacología , Humanos , Ratones