RESUMEN

Pelodiscus sinensis is a common freshwater soft-shell turtle found in China, and is an important aquaculture species. In this study, 20 polymorphic microsatellite primers were developed from the transcriptome. The genetic diversity of three populations of P. sinensis was evaluated, using 72 individuals. The number of alleles per locus ranged from 3 to 26. The observed and expected heterozygosities varied from 0.208 to 0.958, and from 0.302 to 0.963, respectively. The polymorphic information content varied from 0.283 to 0.953. No significant linkage disequilibrium was detected. These markers will be useful for future population genetic studies and molecular breeding of P. sinensis.

Asunto(s)

Tortugas/genética , Animales , Acuicultura , Cruzamiento , Heterocigoto , Desequilibrio de Ligamiento , Repeticiones de Microsatélite , Filogenia , Polimorfismo Genético , Transcriptoma

RESUMEN

Nine microsatellite loci were isolated from the genome of Laodelphax striatellus (Homoptera: Delphacidae) by constructing (TC)(6)(AC)(5) and (AG)(6)(AC)(5) compound SSR-enriched libraries using suppression-PCR procedures. These loci were found to be highly polymorphic, with 13 to 30 alleles per locus in the three populations that we investigated (Jiangsu, Shandong and Zhejiang). The observed and expected heterozygosities ranged from 0.255 to 0.833 and 0.392 to 0.929, respectively. These microsatellite markers can be used for the study of population genetic structure and genetic diversity of L. striatellus.

Asunto(s)

Variación Genética/genética , Hemípteros/genética , Repeticiones de Microsatélite/genética , Animales