RESUMEN
The strategy to sequence repetitive DNA described in this article is based on partial restriction enzyme cleavage. It is an alternative to using nested deletion with exonuclease III or similar enzymes in which progressively more remote regions of the target DNA are brought into range for sequencing by universal primers.
Asunto(s)
ADN/química , Secuencias Repetitivas de Ácidos Nucleicos , Secuencia de Bases , Enzimas de Restricción del ADN , Datos de Secuencia MolecularRESUMEN
For an evaluation of the usefulness of event recording in identifying mechanisms for apparent life-threatening events, 94 infants (median age, 3.1 months; range, 0.5 to 12 months) with a history of two or more apparent life-threatening events of hitherto unknown cause underwent event recording of transcutaneous oxygen pressure, arterial oxygen saturation, (not recorded in all patients), breathing movements, pulse waveforms, electrocardiogram, and instantaneous heart rate. Recordings were triggered by a fall in transcutaneous oxygen pressure to less than 20 mm Hg. During a median duration of event recording of 1.3 months (0.1 to 10 months), 52 events were recorded in 34 patients; 7 of the events had to be excluded because of uninterpretable signals. The following mechanisms were identified in the remaining 30 patients (45 events): a sudden change in skin perfusion but without hypoxemia (6 in 5 patients), hypoxemia induced by an epileptic seizure (6 in 5 patients), hypoxemia induced by suffocation by a parent (4 in 4 patients), and parental fabrication of events and medical history (7 in 6 patients). In the remaining 22 events in 12 patients, the precise mechanism of events could not be identified. Analysis of these as-yet-unexplained events showed prolonged abnormal hypoxemia (a fall in transcutaneous oxygen pressure to between 4 and 18 mm Hg and a fall in arterial oxygen saturation to 5% to 75%), lasting for 40 to 500 seconds in all. Only five of these events involved prolonged (> 20 seconds) apneic pauses, and only four an episode of bradycardia (heart rate < 80 or 60 beats/min). Thus event recording identified various mechanisms of apparent life-threatening events. In the events that remained unexplained, prolonged apneic pauses or episodes of bradycardia were found in only a minority. The identification of hypoxemia therefore may be more relevant to the early detection of these events than the identification of apnea or bradycardia or both. The relevance of these findings with regard to sudden infant death syndrome remains to be determined.
Asunto(s)
Sistema de Conducción Cardíaco , Frecuencia Cardíaca , Hipoxia/fisiopatología , Monitoreo Fisiológico/métodos , Respiración , Apnea/fisiopatología , Monitoreo de Gas Sanguíneo Transcutáneo , Femenino , Humanos , Hipoxia/sangre , Lactante , Recién Nacido , Masculino , Estudios Prospectivos , Muerte Súbita del LactanteRESUMEN
Merozoite surface protein-1 (MSP-1, also referred to as P195, PMMSA or MSA 1) is one of the most studied of all malaria proteins. The protein is found in all malaria species investigated and structural studies on the gene indicate that parts of the molecule are well-conserved. Studies on Plasmodium falciparum have shown that the protein is in a processed form on the merozoite surface, a result of proteolytic cleavage of the large precursor molecule. Recent studies have identified some of these cleavage sites. During invasion of the new red cell most of the MSP1 molecule is shed from the parasite surface except for a small C-terminal fragment which can be detected in ring stages. Analysis of the structure of this fragment suggests that it contains two growth factor-like domains that may have a functional role.
Asunto(s)
Antígenos de Protozoos , Antígenos de Superficie , Plasmodium/metabolismo , Precursores de Proteínas , Proteínas Protozoarias , Secuencia de Aminoácidos , Animales , Antígenos de Protozoos/biosíntesis , Antígenos de Protozoos/química , Antígenos de Protozoos/genética , Antígenos de Superficie/biosíntesis , Antígenos de Superficie/química , Antígenos de Superficie/genética , Calcio/fisiología , Secuencia de Consenso , Eritrocitos/parasitología , Genes Protozoarios , Proteína 1 de Superficie de Merozoito , Datos de Secuencia Molecular , Plasmodium/genética , Plasmodium/crecimiento & desarrollo , Plasmodium/inmunología , Precursores de Proteínas/biosíntesis , Precursores de Proteínas/química , Precursores de Proteínas/genética , Procesamiento Proteico-Postraduccional , Proteínas Protozoarias/biosíntesis , Proteínas Protozoarias/química , Proteínas Protozoarias/genética , Alineación de Secuencia , Homología de Secuencia de Aminoácido , Especificidad de la EspecieAsunto(s)
Niño Hospitalizado , Regulación Gubernamental , Jurisprudencia , Menores , Padres , Cooperación del Paciente , Transfusión Sanguínea , Niño , Maltrato a los Niños , Gobierno Federal , Humanos , Recién Nacido , Rol Judicial , Neoplasias/tratamiento farmacológico , Medición de Riesgo , Estados Unidos , United States Dept. of Health and Human Services , Privación de TratamientoRESUMEN
Las relaciones entre el paciente y el analista, el marco clínico del psicoanálisis, constituyen el núcleo central en torno del cual gira esta obra. Se desarrollan los siguientes temas: la situación clínica, la regresión como fenómeno clínico, la alianza terapéutica, transferencia, contratransferencia, resistencia, etc
RESUMEN
Las relaciones entre el paciente y el analista, el marco clínico del psicoanálisis, constituyen el núcleo central en torno del cual gira esta obra. Se desarrollan los siguientes temas: la situación clínica, la regresión como fenómeno clínico, la alianza terapéutica, transferencia, contratransferencia, resistencia, etc