1.
FEBS Lett
; 381(3): 169-73, 1996 Mar 04.
Artículo
en Inglés
| MEDLINE
| ID: mdl-8601448
RESUMEN
Phosphatidate phosphohydrolase (PAP; EC 3.1.3.4) insensitive to N-ethylmaleimide was partially purified from rat liver membranes by a combination of chromatographic methods, immunoabsorption and glycerol gradient centrifugation. The specific activity was increased more than 600-fold over that of the membrane extract. Enzyme antibodies precipitating more than 80% of PAP were obtained and used for the identification of PAP protein on SDS-polyacrylamide gels employing the immunodetection method of Muilerman et al. [(1982) Anal. Biochem. 120, 46-51]. By this approach PAP was localized as a 31 kDa polypeptide.
Asunto(s)
Hígado/enzimología , Fosfatidato Fosfatasa/aislamiento & purificación , Fosfatidato Fosfatasa/metabolismo , Animales , Membrana Celular/enzimología , Centrifugación por Gradiente de Densidad , Cromatografía de Afinidad , Cromatografía DEAE-Celulosa , Cromatografía en Gel , Electroforesis en Gel de Poliacrilamida , Técnicas de Inmunoadsorción , Cinética , Masculino , Peso Molecular , Conejos , Ratas , Ratas Sprague-Dawley
2.
AMB Rev Assoc Med Bras
; 31(5-6): 76-80, 1985.
Artículo
en Portugués
| MEDLINE
| ID: mdl-2418474