RESUMEN
To study the pathogenesis of X-linked recessive myotubular myopathy (XLMTM), we used a nerve-muscle coculture system which allows the reconstitution of functional motor units in vitro after coupling of human skeletal muscle cells with embryonic rat spinal cord explants. We used three skeletal muscle cell lines derived from subjects with known mutations in the MTM1 gene (two from embryonic tissues, associated with mutations predicted to give a severe phenotype, and one from a neonate still alive at 3 years 6 months and exhibiting a mild phenotype). We compared these three XLMTM muscle cell cultures with control cultures giving special attention to behaviour of living cocultures (formation of the myofibres, contractile activity, survival), expression of muscular markers (desmin, dystrophin, alpha-actinin, troponin-T, myosin heavy chain isoforms), and nerve-muscle interactions (expression and aggregation of the nicotinic acetylcholine receptors). We were unable to reproduce any 'myotubular' phenotype since XLMTM muscle cells behaved like normal cells with regard to all the investigated parameters. Our results suggest that XLMTM muscle might be intrinsically normal and emphasize the possible involvement of the myotubularin-deficient motor neurons in the development of the disease.
Asunto(s)
Diferenciación Celular , Músculo Esquelético/inervación , Músculo Esquelético/patología , Miopatías Estructurales Congénitas/patología , Tejido Nervioso/citología , Animales , Antígenos de Diferenciación/biosíntesis , Diferenciación Celular/fisiología , Supervivencia Celular , Células Cultivadas , Técnicas de Cocultivo , Humanos , Masculino , Contracción Muscular , Músculo Esquelético/embriología , Músculo Esquelético/metabolismo , Mutación , Miofibrillas/metabolismo , Miofibrillas/ultraestructura , Miopatías Estructurales Congénitas/genética , Miopatías Estructurales Congénitas/metabolismo , Tejido Nervioso/embriología , Tejido Nervioso/metabolismo , Fenotipo , Proteínas Tirosina Fosfatasas/genética , Proteínas Tirosina Fosfatasas no Receptoras , Ratas , Receptores Nicotínicos/metabolismo , Médula Espinal/citología , Médula Espinal/embriología , Cromosoma X/genéticaRESUMEN
Friedreich ataxia (FRDA), the most common autosomal recessive ataxia, is characterized by degeneration of the large sensory neurons and spinocerebellar tracts, cardiomyopathy and increased incidence in diabetes. FRDA is caused by severely reduced levels of frataxin, a mitochondrial protein of unknown function. Yeast knockout models as well as histological and biochemical data from heart biopsies or autopsies of FRDA patients have shown that frataxin defects cause a specific iron-sulfur protein deficiency and intramitochondrial iron accumulation. We have recently shown that complete absence of frataxin in the mouse leads to early embryonic lethality, demonstrating an important role for frataxin during mouse development. Through a conditional gene-targeting approach, we have generated in parallel a striated muscle frataxin-deficient line and a neuron/cardiac muscle frataxin-deficient line, which together reproduce important progressive pathophysiological and biochemical features of the human disease: cardiac hypertrophy without skeletal muscle involvement, large sensory neuron dysfunction without alteration of the small sensory and motor neurons, and deficient activities of complexes I-III of the respiratory chain and of the aconitases. Our models demonstrate time-dependent intramitochondrial iron accumulation in a frataxin-deficient mammal, which occurs after onset of the pathology and after inactivation of the Fe-S-dependent enzymes. These mutant mice represent the first mammalian models to evaluate treatment strategies for the human disease.
Asunto(s)
Cardiomiopatías/genética , Ataxia de Friedreich/genética , Neuropatías Hereditarias Sensoriales y Autónomas/genética , Proteínas de Unión a Hierro , Proteínas Hierro-Azufre/metabolismo , Mitocondrias/metabolismo , Fosfotransferasas (Aceptor de Grupo Alcohol)/genética , Animales , Cardiomiopatías/patología , Ataxia de Friedreich/patología , Marcación de Gen , Neuropatías Hereditarias Sensoriales y Autónomas/patología , Ratones , Ratones Mutantes , Mutagénesis , FrataxinaRESUMEN
Oligodendrocytes are glial cells devoted to the production of myelin sheaths. Myelination of the CNS occurs essentially after birth. To delineate both the times of oligodendrocyte proliferation and myelination, as well as to study the consequence of dysmyelination in vivo, a model of inducible dysmyelination was developed. To achieve oligodendrocyte ablation, transgenic animals were generated that express the herpes virus 1 thymidine kinase (HSV1-TK) gene under the control of the myelin basic protein (MBP) gene promoter. The expression of the MBP-TK transgene in oligodendrocytes is not toxic on its own; however, toxicity can be selectively induced by the systemic injection of animals with nucleoside analogs, such as FIAU [1-(2-deoxy-2-fluoro-beta-delta-arabinofuranosyl)-5-iodouracil]. This system allows us to control the precise duration of the toxic insult and the degree of ablation of oligodendrocytes in vivo. We show that chronic treatment of MBP-TK mice with FIAU during the first 3 postnatal weeks triggers almost a total depletion of oligodendrocytes in the CNS. These effects are accompanied by a behavioral phenotype characterized by tremors, seizures, retarded growth, and premature animal death. We identify the period of highest oligodendrocytes division in the first 9 postnatal days. Delaying the beginning of FIAU treatments results in different degrees of dysmyelination. Dysmyelination in MBP-TK mice is always accompanied by astrocytosis. Thus, this transgenic line provides a model to study the events occurring during dysmyelination of various intensities. It also represents an invaluable tool to investigate remyelination in vivo.
Asunto(s)
Arabinofuranosil Uracilo/análogos & derivados , Enfermedades Desmielinizantes/metabolismo , Vaina de Mielina/metabolismo , Oligodendroglía/metabolismo , Proteínas Recombinantes de Fusión/metabolismo , Timidina Quinasa/metabolismo , Animales , Antígenos de Diferenciación/biosíntesis , Northern Blotting , Encéfalo/metabolismo , Encéfalo/patología , Enfermedades Desmielinizantes/inducido químicamente , Enfermedades Desmielinizantes/genética , Modelos Animales de Enfermedad , Femenino , Proteína Ácida Fibrilar de la Glía/biosíntesis , Gliosis/patología , Herpesvirus Humano 1/genética , Hibridación in Situ , Masculino , Ratones , Ratones Transgénicos , Proteína Básica de Mielina/genética , Vaina de Mielina/genética , Vaina de Mielina/patología , Oligodendroglía/efectos de los fármacos , Oligodendroglía/patología , Nervio Óptico/patología , Nervio Óptico/ultraestructura , Regiones Promotoras Genéticas , ARN Mensajero/efectos de los fármacos , ARN Mensajero/metabolismo , Proteínas Recombinantes de Fusión/genética , Nervio Ciático/patología , Nervio Ciático/ultraestructura , Timidina Quinasa/genéticaRESUMEN
BACKGROUND: Polarised trafficking of proteins is critical for normal expression of the epithelial phenotype, but its genetic control is not understood. The regulatory gene lin-26 is essential for normal epithelial differentiation in the nematode Caenorhabditis elegans. To identify potential effectors of lin-26, we characterised mutations that result in lin-26-like phenotypes. Here, we report the phenotypic and molecular analysis of one such mutant line, che-14. RESULTS: Mutations in che-14 resulted in several partially penetrant phenotypes affecting the function of most epithelial or epithelial-like cells of the ectoderm, including the hypodermis, excretory canal, vulva, rectum and several support cells. The defects were generally linked to the accumulation of vesicles or amorphous material near the apical surface, suggesting that secretion was defective. The CHE-14 protein showed similarity to proteins containing sterol-sensing domains, including Dispatched, Patched and NPC1. A fusion protein between full-length CHE-14 and the green fluorescent protein became localised to the apical surface of epithelial cells that require che-14 function. Deletions that removed the predicted transmembrane domains or extracellular loops of CHE-14 abolished apical localisation and function of the protein. CONCLUSIONS: We propose that CHE-14 is involved in a novel secretory pathway dedicated to the exocytosis of lipid-modified proteins at the apical surface of certain epithelial cells. Our data raise the possibility that the primordial function of proteins containing a sterol-sensing domain is to control vesicle trafficking: CHE-14 and Dispatched in exocytosis, Patched and NPC1 in endocytosis.
Asunto(s)
Secuencias de Aminoácidos , Proteínas de Caenorhabditis elegans , Caenorhabditis elegans/genética , Caenorhabditis elegans/metabolismo , Ectodermo/citología , Células Epiteliales/metabolismo , Proteínas del Helminto/genética , Proteínas del Helminto/fisiología , Transporte de Proteínas , Animales , Caenorhabditis elegans/citología , Caenorhabditis elegans/ultraestructura , Polaridad Celular , Vesículas Citoplasmáticas/metabolismo , Vesículas Citoplasmáticas/ultraestructura , Proteínas de Unión al ADN/genética , Proteínas de Unión al ADN/fisiología , Ectodermo/metabolismo , Células Epiteliales/citología , Exocitosis , Proteínas del Helminto/química , Microscopía Electrónica , Microscopía de Contraste de Fase , Neuronas/citología , Neuronas/ultraestructura , Fenotipo , Plásmidos/genética , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/metabolismo , Eliminación de Secuencia/genética , Esteroles/metabolismo , Factores de Transcripción/genética , Factores de Transcripción/fisiologíaRESUMEN
The DAX-1 (NR0B1) gene encodes an unusual member of the nuclear hormone receptor superfamily which acts as a transcriptional repressor. Mutations in the human DAX-1 gene cause X-linked adrenal hypoplasia congenita (AHC) associated with hypogonadotropic hypogonadism (HHG). We have studied the intracellular localization of the DAX-1 protein in human adrenal cortex and mouse Leydig tumor cells and found it to be both nuclear and cytoplasmic. A significant proportion of DAX-1 is associated with polyribosomes and is found complexed with polyadenylated RNA. DAX-1 directly binds to RNA, two domains within the protein being responsible for cooperative binding activity and specificity. Mutations in DAX-1 found in AHC-HHG patients significantly impair RNA binding. These findings reveal that DAX-1 plays multiple regulatory roles at the transcriptional and posttranscriptional levels.
Asunto(s)
Proteínas de Unión al ADN/genética , Proteínas de Unión al ADN/metabolismo , Polirribosomas/metabolismo , ARN Mensajero/metabolismo , Proteínas de Unión al ARN/metabolismo , Receptores de Ácido Retinoico/genética , Receptores de Ácido Retinoico/metabolismo , Proteínas Represoras , Factores de Transcripción/genética , Factores de Transcripción/metabolismo , Corteza Suprarrenal/metabolismo , Hiperplasia Suprarrenal Congénita/genética , Animales , Sitios de Unión , Línea Celular , Núcleo Celular/metabolismo , Citoplasma/metabolismo , Receptor Nuclear Huérfano DAX-1 , Humanos , Hipogonadismo/genética , Células Intersticiales del Testículo/metabolismo , Células Intersticiales del Testículo/patología , Masculino , Ratones , Mutación , Poli A/metabolismo , Biosíntesis de Proteínas , Proteínas de Unión al ARN/genética , Secuencias Repetitivas de Aminoácido , Ribonucleoproteínas/metabolismo , Ribonucleoproteínas/ultraestructura , Temperatura , Neoplasias Testiculares/metabolismo , Neoplasias Testiculares/patologíaRESUMEN
The gene encoding cellular retinol (ROL, vitA)-binding protein type I (CRBPI) has been inactivated. Mutant mice fed a vitA-enriched diet are healthy and fertile. They do not present any of the congenital abnormalities related to retinoic acid (RA) deficiency, indicating that CRBPI is not indispensable for RA synthesis. However, CRBPI deficiency results in an approximately 50% reduction of retinyl ester (RE) accumulation in hepatic stellate cells. This reduction is due to a decreased synthesis and a 6-fold faster turnover, which are not related to changes in the levels of RE metabolizing enzymes, but probably reflect an impaired delivery of ROL to lecithin:retinol acyltransferase. CRBPI-null mice fed a vitA-deficient diet for 5 months fully exhaust their RE stores. Thus, CRBPI is indispensable for efficient RE synthesis and storage, and its absence results in a waste of ROL that is asymptomatic in vitA-sufficient animals, but leads to a severe syndrome of vitA deficiency in animals fed a vitA-deficient diet.
Asunto(s)
Proteínas de Unión al Retinol/genética , Proteínas de Unión al Retinol/metabolismo , Vitamina A/metabolismo , Animales , Femenino , Homeostasis , Hibridación in Situ , Riñón/metabolismo , Riñón/patología , Hígado/metabolismo , Hígado/patología , Pulmón/metabolismo , Pulmón/patología , Masculino , Ratones , Ratones Noqueados , Proteínas de Unión al Retinol/deficiencia , Proteínas Celulares de Unión al Retinol , Vitamina A/administración & dosificación , Deficiencia de Vitamina A/metabolismo , Deficiencia de Vitamina A/patologíaRESUMEN
L1 is a murine multidomain glycoprotein implicated in cell aggregation, fasciculation. neurite outgrowth and synaptogenesis. Laminin, a trimeric polypeptide, is implicated in neuronal survival, growth cone guidance, neurite outgrowth and cell differentiation. Laminin can also interact with the cell adhesion molecule L1. Their expressions were investigated from embryonic day 15 (E15) to adult in the rat hypophysis, and in adult neurohemal zones. Detected in the neural lobe from E17, the L1 immunoreactivity increased during prenatal development and persisted in adulthood mainly related to the neuropeptidergic fibers. Pituicytes were only labelled on the plasmalemma apposed to axons. In the intermediate lobe, L1 appeared at birth on folliculo-stellate cells extensions, constituting a network which densified during postnatal development. L1 is also expressed in all neurohemal areas on neuronal profiles. Laminin was clearly detectable in the hypophysis at E15 before the first blood vessels penetrate the Rathke pouch. At E20, all the basal membranes of the blood vessels were stained. In the intermediate lobe, a spotted laminin immunoreactivity was detected at E21. At this stage, we observed the staining of intercellular spaces and the intracellular labelling of melanotrophs, concerning reticulum or vesicles. The staining of melanotrophs seemed to maintain during adulthood. In contrast with blood vessels of the adult cerebral tissue, adult capillaries of the neural lobe and the others neuro-hemal zones were intensely labelled with the anti-laminin antibody. These results suggest that neurite outgrowth and neurite guidance could be promoted by L1 and laminin in the neurointermediate lobe. The "intercellular tunnels" could also be an important guidance cue for migrating cells in the intermediate lobe. These data also demonstrate that melanotrophic cells. secreting the laminin, have a role in the ontogenesis of the gland. Finally, we suggest that L1 and laminin can collaborate to reinforce "neurons-capillaries" interactions in neurohemal zones.
Asunto(s)
Proteínas Fetales/biosíntesis , Regulación del Desarrollo de la Expresión Génica , Laminina/biosíntesis , Glicoproteínas de Membrana/biosíntesis , Proteínas del Tejido Nervioso/biosíntesis , Moléculas de Adhesión de Célula Nerviosa/biosíntesis , Sistemas Neurosecretores/embriología , Hipófisis/embriología , Animales , Movimiento Celular , Proteínas Fetales/genética , Edad Gestacional , Hipotálamo/embriología , Hipotálamo/crecimiento & desarrollo , Hibridación in Situ , Laminina/genética , Complejo de Antígeno L1 de Leucocito , Glicoproteínas de Membrana/genética , Neovascularización Fisiológica , Proteínas del Tejido Nervioso/genética , Moléculas de Adhesión de Célula Nerviosa/genética , Neuritas/fisiología , Sistemas Neurosecretores/crecimiento & desarrollo , Sistemas Neurosecretores/metabolismo , Hipófisis/irrigación sanguínea , Hipófisis/crecimiento & desarrollo , Hipófisis/inervación , Hipófisis/metabolismo , Ratas , Ratas WistarRESUMEN
Insulin-dependent diabetes mellitus (IDDM) is not a disease of unbridled destruction. The autoimmune attack on pancreatic beta cells has two distinct stages - insulitis and diabetes - and progression of the former to the latter appears to be highly regulated. Identifying the factors controlling this transition has been difficult because it is a complex process that occurs non-universally and asynchronously. We have overcome these difficulties by coupling a simplified TCR transgenic (tg) model of IDDM and the immunosuppressive drug cyclophosphamide (CY). Young BDC2.5 TCR tg mice show insulitis but not diabetes; CY treatment provoked diabetes in 100% of animals with rapid, highly reproducible kinetics. This allowed a detailed temporal analysis of changes in cellular organization and cytokine gene expression within the lesion. The monokines IL-18, IL-12 and TNF-alpha were pivotal, their induction occurring almost immediately and their coordinate action being required for the onset of aggression. Other cytokines with direct toxicity for beta cells, including IL-1 -beta, IL-6 and IFN-gamma, were subsequently induced; in contrast, there was no cellular or molecular evidence of cell contact-mediated mechanisms of beta cell death.
Asunto(s)
Diabetes Mellitus Tipo 1/etiología , Animales , Autoantígenos , Ciclofosfamida/toxicidad , Citocinas/biosíntesis , Citocinas/genética , Diabetes Mellitus Tipo 1/inmunología , Diabetes Mellitus Tipo 1/patología , Modelos Animales de Enfermedad , Humanos , Islotes Pancreáticos/efectos de los fármacos , Islotes Pancreáticos/inmunología , Islotes Pancreáticos/patología , Ratones , Ratones Endogámicos NOD , Ratones Transgénicos , Microscopía Electrónica , ARN Mensajero/genética , ARN Mensajero/metabolismo , Receptores de Antígenos de Linfocitos T/genéticaRESUMEN
The active derivatives of vitamin A (the retinoids) play important and multiple roles in mammalian development and homeostasis. We have previously shown that specific retinoic acid receptors are expressed in the chorioallantoic placenta of the mouse and that among these, RXRalpha is strongly expressed in the developing labyrinthine zone (Sapin, V., Ward, S. J., Bronner, S., Chambon, P., Dollé, P., Dev. Dyn. 208, 199-210, 1997). Here, we show that mouse fetuses with a targeted disruption of the RXRalpha gene develop defects of the chorioallantoic placenta. Both morphological abnormalities and alterations in the expression of molecular markers were found, mostly confined to the labyrinthine zone of placentas from mid-late gestation mutants. This region exhibited edema, abnormal stasis of maternal blood, and signs of disruption of the endothelial layer of fetal vessels. We also detected a reduction in the number of lipid droplets in the trophoblastic layer and abnormal fibrin deposits in the junctional zone of the mutant placentas. These abnormalities most probably result in an impairment of the functional capacities of exchange between the maternal and fetal circulations in the mutant placentas. Thus, placental defects could represent an extraembryonic cause of lethality for RXRalpha null mutant fetuses, in addition to the previously described embryonic cardiac defects.
Asunto(s)
Alantoides/anomalías , Corion/anomalías , Placenta/anomalías , Receptores de Ácido Retinoico/deficiencia , Factores de Transcripción/deficiencia , Alantoides/metabolismo , Animales , Biomarcadores , Corion/metabolismo , Edema , Endotelio Vascular/anomalías , Endotelio Vascular/patología , Femenino , Edad Gestacional , Hemostasis , Ratones , Ratones Noqueados , Placenta/metabolismo , Placenta/ultraestructura , Embarazo , Receptores de Ácido Retinoico/biosíntesis , Receptores de Ácido Retinoico/genética , Receptores X Retinoide , Factores de Tiempo , Factores de Transcripción/biosíntesis , Factores de Transcripción/genéticaRESUMEN
Friedreich ataxia is a progressive neurodegenerative disorder caused by loss of function mutations in the frataxin gene. In order to unravel frataxin function we developed monoclonal antibodies raised against different regions of the protein. These antibodies detect a processed 18 kDa protein in various human and mouse tissues and cell lines that is severely reduced in Friedreich ataxia patients. By immunocytofluorescence and immunocytoelectron microscopy we show that frataxin is located in mitochondria, associated with the mitochondrial membranes and crests. Analysis of cellular localization of various truncated forms of frataxin expressed in cultured cells and evidence of removal of an N-terminal epitope during protein maturation demonstrated that the mitochondrial targetting sequence is encoded by the first 20 amino acids. Given the shared clinical features between Friedreich ataxia, vitamin E deficiency and some mitochondriopathies, our data suggest that a reduction in frataxin results in oxidative damage.
Asunto(s)
Ataxia de Friedreich/metabolismo , Membranas Intracelulares/metabolismo , Proteínas de Unión a Hierro , Proteínas de la Membrana/metabolismo , Mitocondrias/metabolismo , Fosfotransferasas (Aceptor de Grupo Alcohol)/metabolismo , Secuencia de Aminoácidos , Animales , Anticuerpos Monoclonales/inmunología , Formación de Anticuerpos , Células COS , Técnica del Anticuerpo Fluorescente , Células HeLa , Humanos , Proteínas de la Membrana/inmunología , Ratones , Microscopía Inmunoelectrónica , Datos de Secuencia Molecular , Fosfotransferasas (Aceptor de Grupo Alcohol)/inmunología , FrataxinaRESUMEN
The product of the Xl-fli gene, a Xenopus laevis transcription factor of the ets family, specifically expressed in several lineage of migratory cells during Xenopus development (Meyer et al., Int. J. Dev. Biol. 39: 909-919, 1995) was overproduced during Xenopus embryogenesis, upon microinjection of a synthetic transcript in the fertilized egg or in the early embryo. This results in anomalies of the antero-posterior and dorso-ventral polarities, and in tissue differentiation, particularly in the eye- and head cartilage development, as well as erythroid differentiation (absence of erythrocyte differentiation in the circulating blood, often accompanied by ectopic localization of mature erythrocytes, leading to important hemangiomas). Cytological examination reveals at gastrulation the existence of abnormal cells separating the different embryonic layers, suggesting modifications of the cellular adhesion properties. The possible involvement of the fli gene in controlling the dissemination of migratory cells is discussed.
Asunto(s)
Embrión no Mamífero/fisiología , Eritropoyesis , Regulación del Desarrollo de la Expresión Génica , Cabeza/anomalías , Cardiopatías Congénitas , Factores de Transcripción/biosíntesis , Xenopus laevis/embriología , Animales , Diferenciación Celular , Eritrocitos/citología , Femenino , Corazón/embriología , Oocitos/fisiología , Reacción en Cadena de la Polimerasa , Biosíntesis de Proteínas , Transcripción Genética , Xenopus laevis/genéticaRESUMEN
The expression of the Xl-Fli gene, which belongs to the ets family of transcription factors, was studied by whole-mount in situ hybridization during Xenopus embryogenesis. Digoxigenin-labeled antisense RNA probes were synthesized by in vitro transcription and used in the hybridization reaction. In addition to expression in territories invaded by neural crest cells reported earlier (Meyer et al., 1993), we observed Xl-Fli gene expression in a number of regions affected by important cellular migrations and/or epithelium<==>mesenchyme transitions: in the endothelial cells of the heart, in blood vessels, along the pronephric duct migration pathway and at the level of hypophysis. The possibility that the FLI protein is involved in the expression of guidance cues and/or modification of the cellular adhesion properties is discussed. A screening of a promoter library with a consensus sequence, bound by the FLI protein with a high affinity, revealed the presence of putative FLI response elements in a number of genes encoding adhesion molecules or components of the extra-cellular matrix.
Asunto(s)
Regulación del Desarrollo de la Expresión Génica , Factores de Transcripción/genética , Xenopus laevis/embriología , Animales , Secuencia de Bases , Adhesión Celular , Movimiento Celular , Endotelio Vascular/embriología , Corazón/embriología , Hibridación in Situ , Datos de Secuencia Molecular , Hipófisis/embriología , Sondas ARN , ARN sin Sentido , Xenopus laevis/genéticaRESUMEN
Neural cell adhesion molecules (NCAMs) can undergo post-translational modifications, such as the addition of polysialic acid chains, thus generating PSA-NCAMs, which are expressed mainly during development. Since polysialylation considerably modifies NCAM adhesivity, expression of NCAMs and PSA-NCAMs has been investigated in the developing hypophysis by immunohistochemistry. At embryonic day 13 (E13), an antibody against NCAM outlined all cellular profiles in the entire Rathke's pouch; this labelling persisted until adulthood. NCAM expression increased in all lobes during development and concerned all pituitary cell types. In contrast, at E13, PSA-NCAMs were only detected in the neural lobe, solely constituted of pituicytes at this stage, and the tuberal lobe, the only lobe expressing hormonal mRNA at the same stage. PSA-NCAMs expression increased in the neural lobe at E17 with the arrival of the neurosecretory fibres and persisted into adulthood. In the anterior lobe, PSA-NCAMs appeared at E15 where their distribution was similar to that of the differentiating corticotrophic cells; at subsequent stages, their expression extended to the whole anterior lobe. Only two cell types, corticotrophic and somatotrophic cells, remained labelled in the adult gland. In the intermediate lobe, melanotrophic cells never expressed PSA-NCAMs but these were expressed on folliculo-stellate cells at birth, preceding the onset of innervation. These results suggest that NCAMs and PSA-NCAMs play a role in pituitary histogenesis, cell differentiation and neurointermediate lobe innervation.
Asunto(s)
Moléculas de Adhesión Celular Neuronal/biosíntesis , Regulación del Desarrollo de la Expresión Génica , Hipófisis/metabolismo , Ácidos Siálicos/análisis , Animales , Moléculas de Adhesión Celular Neuronal/genética , Diferenciación Celular , Edad Gestacional , Hibridación in Situ , Hipófisis/citología , Hipófisis/embriología , Hipófisis/crecimiento & desarrollo , Hipófisis/inervación , Empalme del ARN , Ratas , Ratas Wistar , Tirosina 3-Monooxigenasa/análisisRESUMEN
The nature of the hormone(s) secreted by the pars tuberalis (PT) is still unknown. This pituitary lobe is mainly formed by specific glandular cells that differ in their ultrastructural features from the other adenohypophysial cell types. Data from the literature indicate the presence of thyroid-stimulating hormone immunoreactivity in the PT-specific cells of the rat and the Djungarian hamster but not of other species, including the mouse and guinea-pig. The PT also encloses variable numbers of pars distalis cells, essentially gonadotrophs that are mainly dispersed in its caudal area. We studied the expression of the glycoprotein hormone alpha-subunit in the PT of the rat, mouse and guinea-pig by in situ hybridization and immunocytochemistry. In situ hybridization, using an oligonucleotide probe complementary to rat cDNA sequence 196-237 revealed the expression of the alpha-subunit gene throughout the PT of the rat and the mouse; in the guinea-pig, the probe labelled no pituitary cells. Light- and electron-microscopic immunocytochemistry demonstrated alpha-subunit immunoreactivity in the secretory granules of the PT-specific cells in the three species examined. These cells did not react with a specific antibody against the beta-subunit of luteinizing hormone, an antibody that labelled scattered gonadotrophs. The present data suggest that hormone(s) produced by the PT-specific glandular cells are, at least partly, related to glycoprotein hormones.
Asunto(s)
Hormonas Glicoproteicas de Subunidad alfa/biosíntesis , Adenohipófisis/metabolismo , Animales , Cricetinae , Sondas de ADN , Femenino , Cobayas , Hibridación in Situ , Masculino , Ratones , Microscopía Inmunoelectrónica , Adenohipófisis/citología , Ratas , Especificidad de la EspecieRESUMEN
The expression of glucocorticoid and D2 dopamine receptors (GR and D2R) during rat pituitary ontogenesis was studied by in situ hybridization (ISH). On early stages, E13-E14, a weak specific signal for GR mRNA was obvious in the whole Rathke's pouch (RP) whereas subsequently, from E17-E18, strong labelling was restricted to the anterior lobe (AL) and the neural lobe (NL). At the same time, D2R mRNAs appeared in the intermediate lobe (IL) and the long isoform of the D2R (D2R 444) was detectable with specific probes. On the postnatal stages, until adult, GR mRNA, if present, was always undetectable in the IL using the conventional ISH technique. These data indicate a possible early regulation of POMC gene expression by glucocorticoid in corticotrophic cells of the AL and by dopamine in the melanotrophic cells of the IL. The possibility of a negative regulation of GR mRNA by dopamine (DA) in the IL as soon as E17 is discussed.
Asunto(s)
Expresión Génica , Hibridación in Situ , Hipófisis/embriología , Hipófisis/crecimiento & desarrollo , Receptores de Dopamina D2/genética , Receptores de Glucocorticoides/genética , Animales , Femenino , Hipófisis/metabolismo , Embarazo , Proopiomelanocortina/genética , ARN Mensajero/metabolismo , Ratas , Ratas WistarRESUMEN
The expression of the alpha-subunit of the glycoprotein hormones was studied in the developing rat pituitary by in situ hybridization and immunocytochemical techniques. The alpha-subunit mRNA labelling was detected in Rathke's pouch at 12 days' gestation (E12); at E13, it was exclusively concentrated in the pars tuberalis primordium (the lateral lobes) where its intensity rapidly increased at following stages. The alpha-subunit was first immunocytochemically detectable in the pars tuberalis at E14. Labelling of the whole pars tuberalis primordium with both techniques indicated that the specific glandular cells were involved. Immunocytochemical detection of bromodeoxyuridine, injected into the pregnant rat 4 h prior to fixation, revealed a total arrest of cell divisions in the pars tuberalis between E13 and E17, which was not the case in the pars distalis or the pars intermedia at any stage. In the pars distalis, the onset of alpha-subunit expression was detected much later than in the pars tuberalis, at E16-17 by in situ hybridization and at E17-18 by immunocytology. The functional significance of the early secretory differentiation of the pars-tuberalis-specific cells remains to be established.
Asunto(s)
Glicoproteínas/biosíntesis , Adenohipófisis/crecimiento & desarrollo , Adenohipófisis/metabolismo , Envejecimiento/metabolismo , Animales , Especificidad de Anticuerpos , Bromodesoxiuridina/farmacología , Femenino , Hormona Folículo Estimulante/biosíntesis , Sistema Hipotálamo-Hipofisario/embriología , Sistema Hipotálamo-Hipofisario/crecimiento & desarrollo , Sistema Hipotálamo-Hipofisario/metabolismo , Inmunohistoquímica , Hibridación in Situ , Hormona Luteinizante/biosíntesis , Adenohipófisis/embriología , Embarazo , ARN Mensajero/biosíntesis , Ratas , Ratas Wistar , Tirotropina/biosíntesisAsunto(s)
Envejecimiento/fisiología , Hormonas Estimuladoras de los Melanocitos/biosíntesis , Hipófisis/fisiología , Animales , Bromocriptina/farmacología , Dopamina/farmacología , Desarrollo Embrionario y Fetal , Femenino , Expresión Génica , Edad Gestacional , Inmunohistoquímica , Hibridación in Situ , Masculino , Hormonas Estimuladoras de los Melanocitos/análisis , Melanóforos , Hipófisis/efectos de los fármacos , Hipófisis/metabolismo , Embarazo , Proopiomelanocortina/biosíntesis , ARN Mensajero/análisis , Radioinmunoensayo , Ratas , Ratas WistarRESUMEN
Muscle regeneration was studied by light and electron microscopy in a case of exercise-induced acute myoglobinuria in a young patient with carnitine-palmityl-transferase deficiency. Various stages of regeneration existed in the foci of necrosis scattered throughout apparently normal muscle. Activated satellite cells, myoblasts and myotubes were found, some of them containing myofibrils. Among the cells accumulating in the necrotic fibres, some apparently contained surviving myonuclei. In some fibres of normal size, developing myofibrils were abundant. Surviving myonuclei may be of significance in the reaction of muscle cells after injury.
Asunto(s)
Ejercicio Físico , Músculos/fisiopatología , Mioglobinuria/etiología , Mioglobinuria/fisiopatología , Regeneración , Preescolar , Humanos , Masculino , Microscopía Electrónica , Músculos/patología , Mioglobinuria/patología , NecrosisRESUMEN
By injecting 6-hydroxydopamine into the pars compacta of the substantia nigra, we produced a hemiparkinsonian rat model in which there is almost complete destruction of the dopaminergic nigrostriatal pathway but sparing of the dopaminergic mesolimbic system. The lesion has been characterized by several criteria: a rotational behavior in response to apomorphine, a complete loss of tyrosine hydroxylase immunoreactivity in the lesioned substantia nigra, a near total depletion of dopamine and metabolites in the striatum ipsilateral to the lesion, and a supersensitivity of the dopamine D2 receptors in the ipsilateral striatum. Dopaminergic striatal deafferentation was accompanied by an increase of D2 receptor mRNA synthesis in the striatum ipsilateral to the lesion, suggesting that the increased D2 receptor density observed after the lesion is due to an increase of the synthesis of receptor molecules. This synthesis appears to be regulated at the transcriptional level.