RESUMEN
A dot immunobinding assay that uses inactivated antigen for the detection of rabies viral antibodies was compared with the rapid fluorescent focus inhibition test. Results of testing pre- and postvaccination sera from humans (n = 33) and canines (n = 22) were identical for both tests. Endpoint titers of positive sera also were approximately the same by both methods. When a mouse monoclonal antibody was used, the dot immunobinding assay antigen was shown to possess detectable rabies virus glycoprotein and core antigens.
Asunto(s)
Anticuerpos Antivirales/análisis , Virus de la Rabia/inmunología , Rabia/diagnóstico , Animales , Perros , Humanos , Técnicas Inmunológicas , Vacunas Antirrábicas/inmunología , VacunaciónRESUMEN
A highly antigenic isolate of Mycoplasma gallisepticum (MG) was utilized in the production of an inactivated, oil-emulsified MG bacterin (MGB). Laboratory tests indicated that the bacterin was capable of protecting chickens from clinical signs of MG caused by intrasinus challenge with the R, S-6, PG-31, or 1150 strain of MG. Vaccinated turkeys also were protected from clinical signs of disease when challenged with MG. Use of the MGB in chickens under laboratory conditions resulted in a reduction in airsacculitis from 44% in nonvaccinates to 10% in vaccinates and further reduced the number of organisms present in the trachea post-challenge. Commercial chickens vaccinated subcutaneously midway or lower in the nape of the neck showed no untoward effects due to the bacterin. Those improperly vaccinated at the base of the skull developed a transient edema around the eye(s). This swelling did not appear to affect the performance of the chickens and had been reabsorbed by the next observation period. Subcutaneous inoculation should be at the mid or lower neck region. Field trials at a commercial egg operation comparing production efficiency showed that chickens vaccinated with the MGB had higher egg production, a greater percentage of eggs graded large and over, a smaller percentage of undergrades, and better feed conversion than chickens vaccinated with a live-culture, low-virulence Conn-F strain vaccine. The results of these studies indicate that the oil-emulsified MG bacterin is safe and highly efficacious.