RESUMEN
Currently, nanomaterials had been used for several applications; one of them is as bio-markers. These nanomaterials contain fluorescent compounds as effective indicators for imaging and other applications in Biotechnology. In previous studies, we proposed a functionalized nanomaterial-based biomarker from silica and Eysenhardtia Polystachia, a medicinal tree known in Mexico as "palo azul" (Kidneywood). Our previous results showed the feasibility of the nanomaterial obtained as bio-marker. In this article, our purpose is to evaluate the effects of extraction solvents on fluorescence of that biomarker. The photoluminescence (PL) effect was evaluated at different pH (4, 7.4 and 8); four extraction solvents, ethanol, methanol, methanol-ethanol and methanol-ethanol-water were evaluated. A molecular dynamics simulation was performed to recognize molecular interaction between the compounds of the extracts with solvent molecules and to investigate the solvent molecules effect on photoluminescence spectra. The results were also compared with rhodamine 6G and we found that, at physiological pH (7.4), the fluorescent-coated silica nanoparticles obtained were also stable. We found that extraction solvents could be used for obtaining different nanomaterials for specific applications, and also found the best extraction solvent for obtaining EP nanomaterials for health care applications, specifically for imaging techniques.
Asunto(s)
Biomarcadores/análisis , Fabaceae/química , Extractos Vegetales/química , Solventes/química , Etanol/química , Fabaceae/metabolismo , Concentración de Iones de Hidrógeno , Metanol/química , Nanopartículas/química , Teoría Cuántica , Rodaminas/química , Dióxido de Silicio/química , Espectrofotometría Ultravioleta , Espectrometría Raman , Electricidad Estática , Agua/químicaRESUMEN
Despite the significant advances in the meniscus tissue engineering field, it is difficult to recreate the complex structure and organization of the collagenous matrix of the meniscus. In this work, we developed a meniscus prototype to be used as substitute or scaffold for the regeneration of the meniscal matrix, recreating the differential morphology of the meniscus by electrospinning. Synthetic biocompatible polymers were combined with the extracellular matrix component, collagen and used to replicate the meniscus. We studied the correlation between mechanical and structural properties of the polymer blend as a function of collagen concentration. Fibers were collected on a surface of a rapidly rotating precast mold, to accurately replicate each sectional morphology of the meniscus; different electro-tissues were produced. Detailed XRD analyses exhibited structural changes developed by electrospinning. We achieved to integrate all these electro-tissues to form a complete synthetic meniscus. Vascularization tests were performed to assess the potential use of our novel polymeric blend for promising meniscus regeneration.
Asunto(s)
Materiales Biocompatibles/química , Polímeros/química , Animales , Colágeno/química , Fuerza Compresiva , Módulo de Elasticidad , Femenino , Riñón/patología , Ácido Láctico/química , Meniscos Tibiales/fisiología , Microscopía Electrónica de Rastreo , Poliésteres , Ratas , Ratas Wistar , Regeneración , Ingeniería de Tejidos , Difracción de Rayos XRESUMEN
Abstract: The tibiofibular syndesmosis provides stability to the ankle mortise. The ankle syndesmosis is compromised in all Weber C type injuries. The radiographic method described by Merle D'Aubigné considers the bony relationships as a measure of syndesmotic widening. We sought to investigate whether the patients with a C type ankle fracture treated with ORIF and placement of a transyndesmal screw have an increment of the tibiofibular space and decrease of the tibiofibular overlap after the transyndesmal screw is removed. Our sample included 52 patients with Weber C ankle fractures treated by ORIF and transyndesmal screw at a level II trauma center. We measured the tibiofibular clear space and tibiofibular overlap in each phase of the treatment. The transyndesmal screw was removed at day 55.56 (± 21.83). We found an increase of the tibiofibular overlap of 0.20 mm (± 2.29, p = 0.532); and 0.21 mm (± 0.97, p = 0.146) in the tibiofibular clear space. The changes of 2.38% in the tibiofibular overlap and 5.29% in the tibiofibular clear space between the postoperative and post-removal periods were not statistically significant. After removal of the syndesmal screw, there is a slight radiographic broadening of the syndesmosis; however, it is small and statistically not significant.
Resumen: La sindesmosis tibioperonea distal proporciona estabilidad a la mortaja del tobillo, viéndose comprometida en todas las lesiones tipo C de Weber. El método radiográfico descrito por Merle D'Aubigné usa las relaciones óseas como una medida del ensanchamiento de la sindesmosis. Investigamos si los pacientes con una fractura de tobillo tipo C tratados con osteosíntesis y colocación de un tornillo transindesmal tienen un incremento del espacio y disminución de la superposición tibioperonea, después de que el tornillo se retira. 52 pacientes con fracturas de tobillo Weber C tratadas mediante osteosíntesis y colocación del tornillo transindesmal en un centro de trauma de nivel II. Medimos el espacio libre tibioperoneo y la superposición en cada fase del tratamiento. El tornillo se retiró el día 55.56 (± 21.83). Encontrando un aumento de la superposición tibioperonea de 0.20 mm (± 2.29, p = 0.532); y 0.21 mm (± 0.97, p = 0.146) en el espacio libre. El aumento del 2.38% en el solapamiento tibioperoneo y 5.29% en el espacio libre entre el postoperatorio y los períodos posteriores al retiro no fueron estadísticamente significativos. Después del retiro del tornillo sindesmal, hay una ligera ampliación radiográfica de la sindesmosis; sin embargo, es pequeña y estadísticamente no significativa.
RESUMEN
The tibiofibular syndesmosis provides stability to the ankle mortise. The ankle syndesmosis is compromised in all Weber C type injuries. The radiographic method described by Merle DAubigné considers the bony relationships as a measure of syndesmotic widening. We sought to investigate whether the patients with a C type ankle fracture treated with ORIF and placement of a transyndesmal screw have an increment of the tibiofibular space and decrease of the tibiofibular overlap after the transyndesmal screw is removed. Our sample included 52 patients with Weber C ankle fractures treated by ORIF and transyndesmal screw at a level II trauma center. We measured the tibiofibular clear space and tibiofibular overlap in each phase of the treatment. The transyndesmal screw was removed at day 55.56 (± 21.83). We found an increase of the tibiofibular overlap of 0.20 mm (± 2.29, p = 0.532); and 0.21 mm (± 0.97, p = 0.146) in the tibiofibular clear space. The changes of 2.38% in the tibiofibular overlap and 5.29% in the tibiofibular clear space between the postoperative and post-removal periods were not statistically significant. After removal of the syndesmal screw, there is a slight radiographic broadening of the syndesmosis; however, it is small and statistically not significant.
La sindesmosis tibioperonea distal proporciona estabilidad a la mortaja del tobillo, viéndose comprometida en todas las lesiones tipo C de Weber. El método radiográfico descrito por Merle DAubigné usa las relaciones óseas como una medida del ensanchamiento de la sindesmosis. Investigamos si los pacientes con una fractura de tobillo tipo c tratados con osteosíntesis y colocación de un tornillo transindesmal tienen un incremento del espacio y disminución de la superposición tibioperonea, después de que el tornillo se retira. 52 pacientes con fracturas de tobillo Weber C tratadas mediante osteosíntesis y colocación del tornillo transindesmal en un centro de trauma de nivel II. Medimos el espacio libre tibioperoneo y la superposición en cada fase del tratamiento. El tornillo se retiró el día 55.56 (± 21.83). Encontrando un aumento de la superposición tibioperonea de 0.20 mm (± 2.29, p = 0.532); y 0.21 mm (± 0.97, p = 0.146) en el espacio libre. El aumento del 2.38% en el solapamiento tibioperoneo y 5.29% en el espacio libre entre el postoperatorio y los períodos posteriores al retiro no fueron estadísticamente significativos. Después del retiro del tornillo sindesmal, hay una ligera ampliación radiográfica de la sindesmosis; sin embargo, es pequeña y estadísticamente no significativa.
RESUMEN
El comportamiento comunitario en la demanda de ayuda en nuestro medio fue analizado mediante los resultados obtenidos en la encuesta comunitaria COPCORD desarrollada en el municipio Lawton. Los datos sociodemográficos resultaron similares a los de la población general. La prevalencia de afecciones reumáticas pesquisada alcanzó el 43,9 por ciento de la serie, con la osteoarttritis como la más prevalerte afección y los niveles de discapacidad elevados. Los pacientes orientan su demandada de atención hacia el facultativo principalmente médico familiar, acude también al hospital donde Ortopédicos, Fisiatras y Reumatólogos le brindanatención. La medicina tradicional es frecuentemente utilizada con el 18.1 por ciento de la serie y no entraña abandono del tratamiento convencionalmente aprobado. La automedicación como práctica desaconsejada resultó frecuente en la población estudiada(AU)
Asunto(s)
Artritis Reumatoide/epidemiología , Medicina ComunitariaRESUMEN
Los primeros reportes en personas en edades gariatricas aparecieron en el 1983 en hijos de mujeres promiscuas, drogadictas y niños que recibieron transfusiones de sangre de donants infectados con el virus del SIDA...(AU)
Asunto(s)
Humanos , Niño , Síndrome de Inmunodeficiencia Adquirida/complicaciones , Enfermedades Reumáticas/complicacionesRESUMEN
En este trabajo se mide la actividad lupica en el lupus eritematoso sistemico mediante determinantes de estos. La actividad lupica se refiere a las manifestaciones clinicas debido a un proceso inflamatorio subyacente y reversible en un momento determinado.(AU)
Asunto(s)
Humanos , Femenino , Lupus Eritematoso Sistémico/diagnóstico , Estadística como AsuntoRESUMEN
OBJECTIVE: Rheumatic diseases are prevalent conditions around the world, but precise information is not easily obtainable in developing countries. The aim of this study was to estimate the prevalence of musculoskeletal complaints in the community in Cuba using the ILAR/COPCORD core questionnaire and published methodologies. METHODS: The ILAR/COPCORD core questionnaire was administered in the form of a home survey to 300 adult subjects. Cases, defined as those with present pain and no trauma, underwent a physical examination and selected laboratory or X ray evaluations. RESULTS: The questionnaire was filled out in a mean time of 8 minutes. Ninety-one subjects had present musculoskeletal pain not related to trauma and 83 had had pain in the past. The most frequently affected regions were the lower back (14%); cervical spine (14%); knee (11.5%) and shoulders (10%). Osteoarthritis was the most common diagnosis (19.6%). A total of 166 subjects sought professional help; 56 were treated by a rheumatologist. Most subjects were satisfied with the results of their medical treatment. CONCLUSIONS: Musculoskeletal symptoms were prevalent in this community. A larger study will be necessary to obtain a better estimate of diagnoses with a low prevalence.