RESUMEN
BACKGROUND: Giant aneurysms are challenging lesions with unacceptable high rates of aneurysm recanalization and rerupture following embolization. Reliable in vivo models are urgently needed to test the performance of new more efficient endovascular devices. MATERIALS AND METHODS: Aneurysms were created in 11 New Zealand white rabbits (4.5-5.5kg): A long venous pouch (length 25-30mm) mimicking the aneurysm sac was derived from the external jugular vein and sutured into a microsurgically created bifurcation between both common carotid arteries. After 4 weeks the rabbits underwent 3T Magnetic resonance angiography (3T-MRA). Exemplary computational fluid dynamics (CFD) simulations were performed to compare the flow conditions of giant rabbit and human aneurysms. We used species-related boundary conditions, in particular, we measured blood viscosity values. Biaxial mechanical tests were performed for the mechanical characterization and comparison. COMPARISON WITH EXISITING METHOD(S): None. RESULTS: No peri- or postoperative mortality was observed. 3T-MRA showed aneurysm patency in 10 out of 11 aneurysms (90.9%). Aneurysm lengths ranged from 21.5-25.6mm and aneurysm necks from 7.3-9.8mm. CFD showed complex flow profiles with multiple vortices in both, rabbit and human aneurysms. Lower blood viscosity values of the rabbit (3.92mPas vs. human 5.34mPas) resulted in considerable lower wall shear stress rates (rabbit 0.38Pa vs. human 1.66Pa). Mechanical tests showed lower stiffness of rabbit aneurysms compared to unruptured human aneurysms. CONCLUSIONS: The proposed model showed favorable aneurysm patency rates, low morbidity and good hemodynamic comparability with complex flow patterns. Biomechanical testing suggests that experimental aneurysms might be even more fragile compared to human aneurysms.
Asunto(s)
Aneurisma , Modelos Animales de Enfermedad , Aneurisma/diagnóstico por imagen , Aneurisma/fisiopatología , Animales , Fenómenos Biomecánicos , Viscosidad Sanguínea , Enfermedades de las Arterias Carótidas/diagnóstico por imagen , Enfermedades de las Arterias Carótidas/fisiopatología , Arteria Carótida Común/diagnóstico por imagen , Arteria Carótida Común/fisiopatología , Arteria Carótida Común/cirugía , Simulación por Computador , Estudios de Factibilidad , Hidrodinámica , Imagenología Tridimensional , Venas Yugulares/cirugía , Angiografía por Resonancia Magnética , Microcirugia/métodos , Modelos Cardiovasculares , Conejos , Reproducibilidad de los ResultadosRESUMEN
BACKGROUND: Thin cerebral aneurysm wall thickness (AWT) is connected to high aneurysm rupture risk. MR imaging of AWT leads to overestimations. The aim of the present study was to quantify MR inaccuracy by comparison with accurate light microscopic measurements. METHODS: In 13 experimental microsurgical bifurcation aneurysms in rabbits, 3 Tesla (3 T)-MR imaging using contrast-enhanced T1 Flash sequences (resolution: 0.4 × 0.4 × 1.5 mm³) was performed. The aneurysms were retrieved immediately after MR acquisition, cut longitudinally, and calibrated photographs were obtained. AWT (dome, neck) and parent vessel thickness (PVT) were measured on the MR images and microscopic photographs by independent investigators. All parameters were statistically compared (Wilcoxon test, Spearman correlation). RESULTS: AWT and PVT could be imaged and measured in all aneurysms with good quality. Comparison with the "real" light microscopic measurements showed a progressive tendency of MR AWT overestimation with smaller AWT: AWT at the dome (0.24 ± 0.06 mm vs. MR 0.30 ± 0.08 mm; p = 0.0078; R = 0.6125), AWT at the neck (0.25 ± 0.07 mm vs. MR 0.29 ± 0.07 mm; p = 0.0469; R = 0.7451), and PVT (0.46 ± 0.06 mm vs. MR 0.48 ± 0.06 mm; p = 0.5; R = 0.8568). CONCLUSION: In this experimental setting, 3 T-MR imaging of cerebral AWT showed unacceptable inaccuracies only below the image resolution threshold. Theoretically, AWT for clinical usage could be classified in ranges, defined by the maximum image resolution.