RESUMEN

An open reading frame, rbcR, was identified 226 bp upstream of rbcAB, i.e., the ribulose 1,5-bisphosphate carboxylase genes expressed in the phototrophic purple bacterium Chromatium vinosum. Several features reveal that rbcR encodes a member of the LysR family of transcriptional regulators, in which an anomalous content of lysine and arginine residues (Lys/Arg anomaly) was found. The expression of rbcR in Escherichia coli as a protein fused to the N-terminal region of beta-galactosidase led to reduced expression of rbcAB. Thus, rbcR is likely to encode a trans-acting transcriptional regulator of rbcAB expression in C. vinosum.

Asunto(s)

Proteínas Bacterianas , Chromatium/genética , Regulación Bacteriana de la Expresión Génica/genética , Genes Reguladores/genética , Factores de Transcripción/genética , Secuencia de Aminoácidos , Arginina/genética , Secuencia de Bases , Chromatium/enzimología , Escherichia coli/metabolismo , Lisina/genética , Datos de Secuencia Molecular , Sistemas de Lectura Abierta/genética , Proteínas Recombinantes de Fusión/biosíntesis , Ribulosa-Bifosfato Carboxilasa/genética , Alineación de Secuencia