RESUMEN
This study investigated how the time interval between the last EMS (netting) and the acute confinement stress (AC stress) at the end of the experiment can influence growth, haematology, blood biochemistry, immunological response, antioxidant system, liver enzymes, and stress response of oscar (Astronotus ocellatus; 5.7 ± 0.8 g). Nine experimental treatments were tested, as follows: Control, Stress28 (EMS in weeks two and eight), Stress27 (EMS in weeks two and seven), Stress26 (EMS in weeks two and six), Stress25 (EMS in weeks two and five), Stress24 (EMS in week two and four), Stress23 (EMS in week two and three), Stress78 (EMS in week seven and eight), and Stress67 (EMS in week six and seven). After the nine-week experimental period, while it was not significant, fish exposed to Stress78 (26.78 g) and Stress67 (30.05 g) had the lowest growth rates. After AC stress, fish exposed to Stress78 (63.33%) and Control (60.00%) showed the lowest survival rate. The Stress78 fish displayed low resilience, illustrated by values of blood performance, LDL, total protein, lysozyme, ACH50, immunoglobin, complement component 4, complement component 3, cortisol, superoxide dismutase, catalase, and alanine aminotransferase. In conclusion, gathering consecutive stress and not enough recovery time in the Stress78 group negatively affected stress responsiveness and the health of oscar.
RESUMEN
OBJECTIVE: Early diagnosis is has a crucial role in both prevention and treatment of asphyxia-related complications. The current study aimed to evaluate the prognostic value of interleukin-6 (IL-6) and hypoxic-ischemic encephalopathy grade in the prediction of mortality and the developmental status of neonates affected by prenatal asphyxia. MATERIALS & METHODS: This cohort study was conducted on 38 term asphyxiated infants at Ghaem hospital, Mashhad, Iran, from 2013 to 2017. The HIE grade and serum IL-6 levels were determined at the time of birth. The developmental status was evaluated using the Denver II test at the end of the two-year follow-up. RESULTS: HIE grade 3 resulted in 83% mortality rate and developmental delay among all survivors. The mean IL-6 level was 2.7 ng/ml in the control group (not affected HIE), which increased up to 29, 175, and 136 ng/ml in those with HIE grades of 1, 2, and 3, respectively. According to the ROC curve analysis, the cut-off level of 24 pg/ml could predict the developmental delay with sensitivity and specificity of 96 and 92%, respectively. CONCLUSION: The IL-6 level and HIE grade are potential prognostic biomarkers for the determination of mortality and morbidity in asphyxiated neonates.