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BACKGROUND: Kanamycin (KAN) residues in animal-derived foods continuously enter the human body, which will pose serious threats to human health such as hearing loss, nephrotoxicity and other complications. Therefore, to sensitively detect KAN residues by a reliable technology is extremely urgent in food quality and safety. Compared with traditional methods being limited by cost and complexity, photoelectrochemical (PEC) biosensors benefit from some merits such as rapid response, excellent sensitivity and good stability. In this study, the construction of a highly efficient PEC platform to realize KAN residues detection is discussed. RESULTS: Herein, a novel p-n heterojunction consisting of flower-like BiOI microspheres and graphite carbon nitride (g-C3N4) nanoflakes was developed to establish a PEC aptasensor for KAN detection at 0 V. The prepared g-C3N4/BiOI heterostructure showed not only significantly enhanced PEC activity due to the larger specific surface area but also greatly increased charge separation efficiency owing to the strong internal electric field. Meanwhile, using g-C3N4/BiOI as a highly efficient photoactive material for binding amine-functionalized aptamers to capture KAN, the photocurrent signals showed a 'turn off' mode to achieve the sensitive detection of KAN. The proposed PEC aptasensor exhibited linear response for KAN from 5 × 10-9 to 3 × 10-7 mol L-1 with a low detection limit of 1.31 × 10-9 mol L-1, and satisfactory recoveries (97.44-107.38 %) were obtained in real food samples analysis. SIGNIFICANCE: This work presented a novel p-n heterojunction-based PEC aptasensor with strong selectivity and stability, rendering it allowed to detect KAN in animal-derived foods including milk, honey and pork. Additionally, the detection range satisfied the MRLs for KAN specified by the national standards, demonstrating the potential application for food analysis. The study provides a new insight into the development of efficient and practical biosensors for antibiotic residues detection.

Asunto(s)

Aptámeros de Nucleótidos , Técnicas Biosensibles , Técnicas Electroquímicas , Grafito , Kanamicina , Aptámeros de Nucleótidos/química , Técnicas Electroquímicas/métodos , Grafito/química , Técnicas Biosensibles/métodos , Kanamicina/análisis , Procesos Fotoquímicos , Límite de Detección , Contaminación de Alimentos/análisis , Compuestos de Nitrógeno/química , Animales , Nitrilos/química , Antibacterianos/análisis , Bismuto

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The intestine and its flora have established a strong link with each other and co-evolved to become a micro-ecological system that plays an important role in human health. Plant polyphenols have attracted a great deal of attention as potential interventions to regulate the intestinal microecology. In this study, we investigated the effects of apple peel polyphenol (APP) on the intestinal ecology by establishing an intestinal ecological dysregulation model using lincomycin hydrochloride-induced Balb/c mice. The results showed that APP enhanced the mechanical barrier function of mice by upregulating the expression of the tight junction proteins at the transcriptional and translational levels. In terms of the immune barrier, APP downregulated the protein and mRNA expression of TLR4 and NF-κB. As for the biological barrier, APP promoted the growth of beneficial bacteria as well as increasing the diversity of intestinal flora. In addition, APP treatment significantly increased the contents of short-chain fatty acids in mice. In conclusion, APP can alleviate intestinal inflammation and epithelial damage as well as inducing potentially beneficial changes in the intestinal microbiota, which helps to reveal the potential mechanisms of host-microbial interactions and polyphenol regulation of intestinal ecology.

Asunto(s)

Microbioma Gastrointestinal , Malus , Humanos , Ratones , Animales , FN-kappa B/genética , Antibacterianos/farmacología , Malus/metabolismo , Receptor Toll-Like 4/genética , Proteínas de Uniones Estrechas/metabolismo , Polifenoles/farmacología , Disbiosis/inducido químicamente , Disbiosis/tratamiento farmacológico , Disbiosis/genética

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PURPOSE: After patent foramen ovale interventional closure, puncture of the interatrial septum through the occluder is difficult but sometimes needed for further interventional treatment. This paper presents findings from an in vivo experimental study of a reserved atrial septal puncture area patent foramen ovale occluder. MATERIALS AND METHODS: A patent foramen ovale model was established in canines using trans-septal puncture of the fossa ovale and high-pressure balloon dilation. Then, patent foramen ovale closure was performed with a reserved atrial septal puncture area and all canines were raised for 3 months. Then, the occluder was crossed and left atrial angiography was performed on the septal area with the occluder. Finally, DSA angiography, echocardiography, and histology were used to evaluate the performance and feasibility of the reserved atrial septal puncture area. RESULTS: A patent foramen ovale model was successfully established in 10 canines using the atrial septal puncture method. The average diameter of the patent foramen ovale was 3.77 ±0.19 mm, and the patent foramen ovale was successfully closed in all canines using a reserved atrial septal puncture area. As assessed using transoesophageal echocardiography, the new occluder exhibited an ideal position and was occluded entirely without a residual shunt intraoperatively and postoperatively. A 100% success rate of atrial septum puncture was achieved across the new occluder. The occluders were completely endothelialised 3 months post-implantation. CONCLUSIONS: The reserved atrial septal puncture area was effective in patent foramen ovale closure and exhibited positive sealing performance and biological compatibility. Trans-septal puncture was feasible and effective after reserved atrial septal puncture area patent foramen ovale closure.

Asunto(s)

Tabique Interatrial , Foramen Oval Permeable , Dispositivo Oclusor Septal , Humanos , Animales , Perros , Foramen Oval Permeable/diagnóstico por imagen , Foramen Oval Permeable/cirugía , Tabique Interatrial/diagnóstico por imagen , Tabique Interatrial/cirugía , Ecocardiografía , Ecocardiografía Transesofágica , Punciones , Cateterismo Cardíaco , Resultado del Tratamiento

RESUMEN

Purpose: To evaluate a noval bilateral asymmetric single-rivet occluder with reserved interatrial septal puncture area for treating patent foramen ovale (PFO). Materials and methods: The study established a pig model of patent foramen ovale (PFO) by puncturing the oval fossa and then performing high-pressure balloon dilation. A specially designed bilateral asymmetric occluder for the reserved interatrial septal puncture area was then. used to close the PFO through catheter-based intervention. The pigs were kept for 3 months before undergoing a second catheter-based intervention, involving interatrial septal puncture using a newly developed occluder in the reserved interatrial septal puncture area. During 6 months, the experimental pigs underwent assessment using digital subtraction angiography (DSA), echocardiography, and histological evaluation. Results: A patent foramen ovale (PFO) model was successfully established in 6 pigs using the puncture atrial septum high-pressure balloon dilation method. The diameter of the unclosed PFO was measured (3.56 ± 0.25 mm). Using the newly developed occluder device, all 6 pigs with unclosed PFO underwent successful catheter-based closure surgeries, with intraoperative and postoperative transesophageal echocardiography showing excellent device positioning and complete closure without residual shunting. After 3 months of implantation, the catheter-based interatrial septal puncture was performed through the reserved interatrial septal puncture area, and all procedures were successful. Immediately following euthanasia, a histological examination revealed intact and undamaged occluder devices with visible puncture holes in the reserved interatrial septal puncture area. No fracture of the nitinol wire was observed, and the surface of the occluder device showed coverage of endothelial and connective tissues. Utilizing a bilateral asymmetric single-rivet occluder device implanted through the reserved interatrial septal puncture area has proven effective in closing PFO. After implantation, the occluder device allows subsequent interatrial septal puncture procedures through the reserved area. Conclusion: The novel occluder device demonstrated excellent closure performance, biocompatibility, and puncturability in the experiment. This indicates the feasibility of conducting further catheter-based interventions on the interatrial septum.

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Virtual reality (VR) and augmented reality (AR) have widespread applications. The vergence-accommodation conflict (VAC), which causes 3D visual fatigue, has become an urgent challenge for VR and AR displays. Alvarez lenses, with precise and continuously tunable focal length based on the lateral shift of its two sub-elements, are a promising candidate as the key electro-optical component in vari-focal AR display systems to solve the VAC problem. In this paper, we propose and fabricate a compact Alvarez lens based on planar polymetric liquid crystal Pancharatnam-Berry optical elements. It can provide continuous diopter change from -1.4 D to 1.4 D at the wavelength of 532 nm with the lateral shift ranging from -5 mm to 5 mm. We also demonstrate an AR display system using this proposed Alvarez lens, where virtual images are augmented on the real world at different depths.

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The cytogenetic abnormalities and molecular mutations involved in acute myeloid leukemia (AML) lead to unique treatment challenges. Although adoptive T-cell therapies (ACT) such as chimeric antigen receptor (CAR) T-cell therapy have shown promising results in the treatment of leukemias, especially B-cell malignancies, the optimal target surface antigen has yet to be discovered for AML. Alternatively, T-cell receptor (TCR)-redirected T cells can target intracellular antigens presented by HLA molecules, allowing the exploration of a broader territory of new therapeutic targets. Immunotherapy using adoptive transfer of WT1 antigen-specific TCR-T cells, for example, has had positive clinical successes in patients with AML. Nevertheless, AML can escape from immune system elimination by producing immunosuppressive factors or releasing several cytokines. This review presents recent advances of antigen-specific TCR-T cells in treating AML and discusses their challenges and future directions in clinical applications.

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We propose a novel optical design to tailor the angular distribution of a micro-LED (µLED) display system and use vehicle display as an example to illustrate the design principles. The display system consists of a µLED array with a tailored LED structure, a small formfactor compound parabolic concentrator (CPC) system, and a functional engineered diffusor. It provides high efficiency, high peak brightness, and small formfactor. In the design process, a mix-level optical simulation model, including the angular distribution of polarized emission dipole (dipole emission characteristics), Fabry-Perot cavity effect (wave optics), and light propagation process (ray optics), is established to analyze the angular distribution of µLEDs. Such an optical design process from dipole emission to display radiation pattern can be extended to other µLED display systems for different applications.

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With rapid advances in high-speed communication and computation, augmented reality (AR) and virtual reality (VR) are emerging as next-generation display platforms for deeper human-digital interactions. Nonetheless, to simultaneously match the exceptional performance of human vision and keep the near-eye display module compact and lightweight imposes unprecedented challenges on optical engineering. Fortunately, recent progress in holographic optical elements (HOEs) and lithography-enabled devices provide innovative ways to tackle these obstacles in AR and VR that are otherwise difficult with traditional optics. In this review, we begin with introducing the basic structures of AR and VR headsets, and then describing the operation principles of various HOEs and lithography-enabled devices. Their properties are analyzed in detail, including strong selectivity on wavelength and incident angle, and multiplexing ability of volume HOEs, polarization dependency and active switching of liquid crystal HOEs, device fabrication, and properties of micro-LEDs (light-emitting diodes), and large design freedoms of metasurfaces. Afterwards, we discuss how these devices help enhance the AR and VR performance, with detailed description and analysis of some state-of-the-art architectures. Finally, we cast a perspective on potential developments and research directions of these photonic devices for future AR and VR displays.

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Non-mechanical beam steerers with lightweight, compact, high-efficiency, high-precision, and/or large-angle are pivotal for light detection and ranging (LiDAR) of autonomous vehicles, eye-tracking for near-eye displays, microscopy, optical tweezers, and high-precision three-dimensional (3D) printing. However, even the most matured optical phased array can only provide quasi-continuous, efficient beam steering within a small angle range. A telescope module with an angle magnification function can be coupled to enlarge the steering range or precision. But obtaining a compact, low-cost, lightweight, high-quality telescope module with conventional optics remains challenging. Patterned liquid crystal-based planar optical elements offer great design freedom for manipulating the phase profile of light in 2D space. Owing to the advantages of high efficiency, thinness, low cost, easy processing, flexibility, and response to environmental stimuli, a plethora of high-quality optical devices have been demonstrated. Here, a miniature planar telescope mediated by liquid crystal polymers is proposed to offer angle magnification independent of incident spatial location. It consists of two cascaded liquid crystal planar optical elements, each performing a predefined mathematical transformation. By this concept, planar optical elements are fabricated using a new exposure method and assembled into planar telescopes with different magnification factors. Within the incident field range, over 84.6% optical efficiency is achieved with small wavefront distortion. Such a miniature planar telescope shows the potential of cascaded liquid crystal planar optical elements for realizing functionalities that cannot be fulfilled by single optical elements, and enables lightweight, low loss, passive optical transmitters for widespread applications.

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We propose a glasses-like augmented reality (AR) display with an extended field-of-view (FOV) using a liquid crystal polarization-dependent combiner (PDC). Such a PDC consists of two polarization volume lenses (PVLs) that are based on patterned liquid crystals to selectively control the beam path according to the right-handed or left-handed circularly polarized light. By encoding the left and right half of the FOV into two orthogonal polarization states, the overall horizontal FOV can be doubled while maintaining an ultrathin and flat form factor. Based on this multiplexing concept, the FOV can be further extended by integrating more PVLs with different diffraction angles. The proposed configuration with polarization-time multiplexing provides a promising solution for overcoming the limited FOV issue in AR displays.

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Chiral liquid crystals are self-organized Bragg reflectors which respond to circularly polarized light. Manipulation of the chiral structure has aroused great research interest. The x-y plane two-dimensional patterning of chiral liquid crystals leads to reflective planar optics, and the z-axis modulation results in a variety of photonic bandgap controls. Here, the optical properties of even-number left- and right-handed chiral liquid crystal stacks are investigated, with emphasis on the linear polarization response. Under certain conditions, a linearly polarized incidence can result in a linearly polarized reflected light. More intriguingly, the linear polarization has different forms of response to thick and thin chiral liquid crystal sublayers and responds to the rotation of liquid crystal alignment. Based on the peculiar polarization response, a new type of wavelength-response camouflage and anti-counterfeiting is conceptually proposed, which can hide two different images simultaneously within a small spectral range. Our work paves the way for three-dimensional manipulation of chiral liquid crystals and enlightens novel applications.

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We demonstrate a large-diffraction-angle two-dimensional (2D) grating based on cholesteric liquid crystal (CLC). One dimension is a polarization volume grating (PVG) working in the Bragg regime, which is produced by a patterned photoalignment layer. The other dimension is a CLC grating working in the Raman-Nath regime, which is introduced by CLC self-assembly under a weak anchoring energy condition. The condition for the coexistence of the CLC Raman-Nath grating (RNG) and PVG is analyzed, and the efficiency and grating period of the CLC RNG are also characterized. Potential application of this 2D grating for enlarging the eyebox of augmented reality displays is discussed.

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We develop an optical model including the glare effect in the human vision system to analyze the halo effect of high-dynamic-range (HDR) mini-LED backlit liquid crystal displays (LCDs). In our model, an objective function is first introduced to evaluate the severity of the halo effect with different image contents. This function is further combined with PSNR to establish a new evaluation metric to analyze the image quality affected by the halo effect. A subjective visual experiment is also conducted to verify the above-mentioned evaluation metrics. In addition, we analyze the influence of ambient environment (viewing angle and ambient light illuminance) on the halo effect. After considering the requirements on local dimming zones, dynamic contrast ratio, gamma shift, and color shift for practical applications, we find that fringe-field-switching mode is a strong contender for the mini-LED backlit LCD system.

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Color-converted micro-light-emitting diode (micro-LED) displays with wide color gamut, high ambient contrast ratio, and fast response time are emerging as a potentially disruptive technology. However, due to limited optical density and thickness of the color-conversion film, the blue light leakage and low color-conversion efficiency still hinder their widespread applications. In this paper, we demonstrate a patterned cholesteric liquid crystal (CLC) polymer film with two special optical functionalities. On the green and red sub-pixels, the corresponding planar CLC texture acts as a distributed Bragg reflector for the blue light, which in turn improves the color conversion efficiency and expands the color gamut. On the blue sub-pixels, the corresponding focal-conic CLC texture acts as light scattering medium, which helps to reduce the angular color shift. Further analysis reveals that the patterned CLC film can alleviate the crosstalk between green and blue color filters. Therefore, compared to the display system without such a CLC film, our proposed device structure increases the color conversion efficiency by 143% (at ~90% Rec. 2020) and reduces average angular color shift Δu'v' from 0.03 to 0.018 at the viewing angle with the most severe color shift. Such a patterned CLC film is applicable to all kinds of color-conversion display systems, including organic and inorganic phosphors.

RESUMEN

Liquid-crystal (LC)-based ultrathin flat optical elements (FOEs) exhibit several attractive properties, such as a high degree of optical tunability, strong polarization selectivity, nearly 100% diffraction efficiency, and a simple fabrication process. Investigating the alignment patterning of LC-FOEs to diversify their performance has attracted broad interest in the optics field. In this mini-review, we start from the photoalignment (PA) process and then dive into device structures and performances. By generating and recording the desired polarization fields on the PA layer, the LC molecules will follow the recorded patterns and establish the phase profiles for different functionalities, such as gratings and lenses. Because of the polarization dependency, LC-FOEs have found useful applications in near-eye displays. Understanding the interactions between the PA mechanism and LC molecules helps to optimize the device performance for novel optical systems.

RESUMEN

The mechanisms of highland barley whole grain (BWG) with rich phenolics on obese db/db mice were investigated in this study. Oral consumption of BWG reduced food intake, body weight, organ/body weight indexes of liver and fat, levels of serum and hepatic lipids, liver injury, and oxidative stress. Furthermore, BWG recovered the disorder of cecal microbiota by augmenting the Bacteroidetes/Firmicutes ratio and Alistipes abundance and decreasing the abundances of Bacteroides and Desulfovibrionaceae to modulate lipid metabolism-related genes. BWG inhibited fatty acid biosynthesis via upregulating the phosphorylation of AMP-activated protein kinase α, while downregulating sterol regulatory element binding protein-1c, fatty acid synthase (FAS), and stearoyl-CoA desaturase 1 levels. BWG also significantly downregulated miRNA-122, miRNA-33, miRNA-34a, and miRNA-206 levels. Accordingly, BWG exhibited hypolipidemic potential through modulating cecal microbiota, AMPK/SREBP-1c/FAS pathway, and related miRNAs, triggering the alleviation of dyslipidemia. These findings suggested BWG as an effective candidate to ameliorate the symptoms of hyperlipidemia.

Asunto(s)

Proteínas Quinasas Activadas por AMP/metabolismo , Ciego/microbiología , Microbioma Gastrointestinal , Hordeum/metabolismo , Hiperlipidemias/dietoterapia , Receptores de Leptina/deficiencia , Proteínas Quinasas Activadas por AMP/genética , Animales , Bacterias/clasificación , Bacterias/genética , Bacterias/aislamiento & purificación , Ciego/metabolismo , Modelos Animales de Enfermedad , Ácido Graso Sintasas/genética , Ácido Graso Sintasas/metabolismo , Humanos , Hiperlipidemias/genética , Hiperlipidemias/metabolismo , Hiperlipidemias/microbiología , Hígado/metabolismo , Masculino , Ratones , Ratones Noqueados , MicroARNs/genética , MicroARNs/metabolismo , Receptores de Leptina/genética , Semillas/metabolismo , Proteína 1 de Unión a los Elementos Reguladores de Esteroles/genética , Proteína 1 de Unión a los Elementos Reguladores de Esteroles/metabolismo

RESUMEN

As one of the most promising candidates for next-generation mobile platform, augmented reality (AR) and virtual reality (VR) have potential to revolutionize the ways we perceive and interact with various digital information. In the meantime, recent advances in display and optical technologies, together with the rapidly developing digital processers, offer new development directions to advancing the near-eye display systems further. In this perspective paper, we start by analyzing the optical requirements in near-eye displays poised by the human visual system and then compare it against the specifications of state-of-the-art devices, which reasonably shows the main challenges in near-eye displays at the present stage. Afterward, potential solutions to address these challenges in both AR and VR displays are presented case by case, including the most recent optical research and development, which are already or have the potential to be industrialized for extended reality displays.

RESUMEN

We demonstrate a full-color high dynamic range head-up display (HUD) based on a polarization selective optical combiner, which is a three-layer cholesteric liquid crystal (CLC) film. Such a CLC film has three reflection bands corresponding to the three primary colors. A key component in our HUD system is a polarization modulation layer (PML) consisting of a twisted-nematic LC polarization rotator sandwiched by two quarter-wave plates. This spatially switchable PML generates opposite polarization states for the displayed image and its background area. Thus, this optical combiner reflects the displayed image to the observer and transmits the background noise, making the black state darker. Furthermore, by matching the reflection spectra of the optical combiner with the colors of the display panel, the bright state gets brighter. Therefore, both bright state and dark state are improved simultaneously. Our experimental results show that the dark state of the new HUD is lowered by 3x and bright state is boosted by 2.5x. By applying antireflection coating to the optical components and optimizing the degree of polarization, our simulation results indicate that the dynamic range can be improved by ∼50x (17 dB). Potential applications of the proposed HUDs for improving the driver's safety are foreseeable.

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A standing wave polarization holography setup is proposed to generate the desired polarization field for fabricating both on-axis and off-axis liquid crystal Pancharatnum-Berry phase lenses. Compared to other interference exposure setups, standing wave interferometry can double the polarization field amplitude because it does not require a beam splitter. Moreover, the optical axis angle of the lenses can be easily adjusted without realigning the optical setup. Based on the design, we first theoretically derive the polarization field distribution. In the experiment, we build the recording optical system and fabricate a series of on-axis and off-axis lenses. Further optical characterization proves the high diffraction efficiency of the fabricated lenses.

RESUMEN

Nanostructured plasmonic materials can lead to the extremely compact pixels and color filters needed for next-generation displays by interacting with light at fundamentally small length scales. However, previous demonstrations suffer from severe angle sensitivity, lack of saturated color, and absence of black/gray states and/or are impractical to integrate with actively addressed electronics. Here, we report a vivid self-assembled nanostructured system which overcomes these challenges via the multidimensional hybridization of plasmonic resonances. By exploiting the thin-film growth mechanisms of aluminum during ultrahigh vacuum physical vapor deposition, dense arrays of particles are created in near-field proximity to a mirror. The sub-10-nm gaps between adjacent particles and mirror lead to strong multidimensional coupling of localized plasmonic modes, resulting in a singular resonance with negligible angular dispersion and ∼98% absorption of incident light at a desired wavelength. The process is compatible with arbitrarily structured substrates and can produce wafer-scale, diffusive, angle-independent, and flexible plasmonic materials. We then demonstrate the unique capabilities of the strongly coupled plasmonic system via integration with an actively addressed reflective liquid crystal display with control over black states. The hybrid display is readily programmed to display images and video.