RESUMEN
Nowadays, continuous efforts have been devoted to searching highly efficient electrochemiluminescence (ECL) emitters for applications in clinical diagnosis and food safety. In this work, triazinyl-based hydrogen bond organic frameworks (Tr-HOFs) were synthesized by N···H hydrogen bond self-assembly aggregation, where 6,6'-(1,4-phenylene)bis(1,3,5-triazine-2,4-diamine) (phenyDAT) was prepared via the cyclization reaction and behaved as a novel ligand. Impressively, the resulting Tr-HOFs showed strong ECL responses with highly enhanced ECL efficiency (21.3%) relative to the Ru(bpy)32+ standard, while phenyDAT hardly showed any ECL emission in aqueous phase. The Tr-HOFs innovatively worked as a new ECL luminophore to construct a label-free biosensor for assay of kanamycin (Kana). Specifically, the ECL response greatly weakened upon assembly of captured DNA with ferrocene (cDNA-Fc) onto the Tr-HOFs-modified electrode, while the ECL signals were adversely recovered by releasing linked DNA (L-DNA) from double-stranded DNA (dsDNA, hybridization of aptamer DNA (aptDNA) with L-DNA) due to the specific recognition of Kana with the aptDNA combined by the linkage of L-DNA and cDNA-Fc on the electrode. The as-built sensor showed a broadened linear range (1 nM-10 µM) and a limit of detection (LOD) down to 0.28 nM, which also displayed satisfactory results in the analysis of Kana in the milk and diluted human serum samples. This work offers a novel pathway to design an ECL emitter with organic molecules, holding great promise in biomedical analysis and food detection.
Asunto(s)
Técnicas Biosensibles , Técnicas Electroquímicas , Humanos , Enlace de Hidrógeno , Límite de Detección , Mediciones LuminiscentesRESUMEN
This study aimed to qualify the potential water sources and their responses to seasonal precipitations for the system of Platycladus orientalis and Vitex negundo var. heterophylla by IsoSource model based on stable hydrogen and oxygen isotopic analysis in Jiufeng Mountain area of Beijing. The results showed that the 18O of water from 0-20 cm soil layer was enriched, whereas that was depleted as the soil layer deepened. P. orientalis used water mainly from 0-30 cm soil la-yer, being composed of rainwater 2-3 days before at the beginning of dry season. The water absorbed by P. orientalis and V. negundo sourced from 0-10 cm and 10-30 cm soil layer, which was fed on recent rainwater at the end of dry season. In wet season P. orientalis mainly accessed the soil water (from 0-40 cm layer, 59.3%) and recent rainwater (12.5%), while V. negundo drank the water from 0-30 cm soil layer derived from recent heavy rain. P. orientalis actively uptook the deeper soil water with time, until the end of growing season (November), its available water was from 60-80 cm soil layer and sourced from the rainwater happened 2-3 days before. Meanwhile, V. negundo completed its growing cycle and was on the brink of death. This system faced less competition for water use, stating its vertical water availability for climate adaptation in this region, which could reduce water and soil loss and minimize the instantaneous damage under heavy rainstorm attack.