RESUMEN
To enhance the efficiency of the Stinger Polycrystalline Diamond Compact (PDC) cutter in breaking hard rocks, this study focuses on optimizing the cutter intrusion-cutting rock breaking parameters. A numerical calculation model for the rotational breaking of granite by a Stinger PDC cutter was established. A comprehensive statistical examination was performed to assess the influence of various factors on intrusion ability (IA), tangential force (TF), and mechanical specific energy (MSE). The Taguchi method was used to determine the optimal settings for each factor, while analysis of variance was employed to assess the significance and relative impact of these factors on the target outcomes. In addition, the multi-objective function was optimized using the gray relational analysis method. The primary process parameters obtained for the various performance characteristics are the cone top angle (α), the cone top radius (r), the cutter diameter (d), the cutter back inclination angle (ß), and weight on bit (P). The impact ratios of these parameters are 6.20%, 7.66%, 3.93%, 17.20%, and 65.02%, respectively. The optimal geometrical parameters are α = 60°, r = 2 mm, and d = 15 mm, while the optimal working parameters are ß = 30° and P = 800 N. In the optimal case, IA and MSE were reduced by 55.335% and 15.809%, respectively, compared to the initial case. Despite a 15.706% increase in TF, the overall GRG increased for all three evaluation criteria, with an overall increase in efficiency of 18.229%. The results of this paper can provide guidance for the design of Stinger cutter PDC drill bits.
RESUMEN
Shikonin, a botanical drug extracted from Lithospermum erythrorhizon, exhibits anti-cancer effects in various cancer cell lines. However, the mechanisms underlying these effects have not been completely elucidated yet. Here, we showed that Shikonin induces apoptosis and autophagy in A375 cells and inhibits their proliferation. Shikonin caused G2/M phase arrest through upregulation of p21 and downregulation of cyclin B1. Shikonin significantly triggered ER stress-mediated apoptosis by upregulating the expression of p-eIF2α, CHOP, and cleaved caspase-3. It also induced protective autophagy by activating the p38 pathway, followed by an increase in the levels of p-p38, LC3B-II, and Beclin 1. Upon suppression of autophagy by 3-methyladenine, Shikonin-induced apoptosis was enhanced in A375 cells. Moreover, after pretreatment with N-acetyl-cysteine, Shikonin increased the production of reactive oxygen species that are involved in regulating ER stress-mediated apoptosis and p38-activated autophagy, as evidenced by the reversion of cell viability and apoptosis and a decrease in p-eIF2α, CHOP, p-p38, LC3B-II, and Beclin 1 levels. Thus, we demonstrated that Shikonin induced apoptosis and autophagy in A375 cells via the activation of ROS-mediated ER stress and p38 pathways, indicating that Shikonin can serve as a potential agent for human melanoma therapy.