RESUMEN

Medicine mulberry (Morus nigra) mainly distributed in southern areas of Xinjiang Uighur Autonomous Region and introduced by grafting, is a unique Morus species, whose plant number is little. As a traditional herbal medicine, medicine mulberry with high levels of secondary metabolites has important values of scientific research and utilization. In order to solve the introduction problems for medicine mulberry, we have established its rapid propagation system through tissue culture since 2011. The shoots of medicine mulberry through tissue culture were transplanted into the field to carry out an introduction experiment. Here, we firstly reported that the growth status and pest and disease occurrence of medicine mulberry in the field of Chongqing and found that the medicine mulberry through tissue culture had well-developed root system, it showed better growth than medicine mulberry by grafting technique, and Pseudodendrothrips moil was a major pest of medicine mulberry. The introduction technique for medicine mulberry established successfully in this study could lay the foundation for large-scale cultivation and high efficiency utilization of medicine mulberry.

Asunto(s)

Agricultura/métodos , Morus/crecimiento & desarrollo , Plantas Medicinales/crecimiento & desarrollo , Medicamentos Herbarios Chinos , Técnicas de Cultivo de Tejidos

RESUMEN

HWTX-I is a peptide neurotoxin purified from the crude venom of the Chinese bird Spider Selenocosmia Huwena, which has analyesic activity. rHWTX-I expressed by P. pastoris and secreted to culture supernatant was first precipitated by (NH4)2SO4, then it was isolated and desalted by ultrofiltration following by ion exchange chromatography of CM column, after reverse phase HPLC of C18 column and vacuum drying, the pure HWTX-I protein was obtained which was proved to be recombinant HWTX-I by Tricine SDS-PAGE, MALDI-TOF mass spectrometry, amino acid composition analysis, the N-terminal amino acid sequence and its biological activity. The final yield of the purified HWTX-I was about 80 mg/L accounting for 23.6% of its total secretory proteins.

Asunto(s)

Expresión Génica , Neurotoxinas/genética , Venenos de Araña/genética , Secuencia de Aminoácidos , Animales , Cromatografía Líquida de Alta Presión/métodos , Cromatografía por Intercambio Iónico/métodos , Medios de Cultivo , Concentración de Iones de Hidrógeno , Masculino , Ratones , Datos de Secuencia Molecular , Unión Neuromuscular/efectos de los fármacos , Neurotoxinas/aislamiento & purificación , Neurotoxinas/farmacología , Pichia , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/aislamiento & purificación , Proteínas Recombinantes de Fusión/farmacología , Proteínas de Reptiles , Túbulos Seminíferos/efectos de los fármacos , Análisis de Secuencia de Proteína , Venenos de Araña/aislamiento & purificación , Venenos de Araña/farmacología , Arañas , Transmisión Sináptica/efectos de los fármacos , Factores de Tiempo