RESUMEN
Elevated levels of cadmium (Cd) have the ability to impede plant development. Aldo-keto reductases (AKRs) have been demonstrated in a number of plant species to improve tolerance to a variety of abiotic stresses by scavenging cytotoxic aldehydes; however, only a few AKRs have been identified to improve Cd tolerance. The OsAKR1 gene was extracted and identified from rice here. After being exposed to Cd, the expression of OsAKR1 dramatically rose in both roots and shoots, although more pronounced in roots. According to a subcellular localization experiment, the nucleus and cytoplasm are where OsAKR1 is primarily found. Mutants lacking OsAKR1 exhibited Cd sensitive phenotype than that of the wild-type (WT) Nipponbare (Nip), and osakr1 mutants exhibited reduced capacity to scavenge methylglyoxal (MG). Furthermore, osakr1 mutants exhibited considerably greater hydrogen peroxide (H2O2) and malondialdehyde (MDA) levels, and increased catalase (CAT) activity in comparison to Nip. The expression of three isomeric forms of CAT was found to be considerably elevated in osakr1 mutants during Cd stress, as demonstrated by quantitative real-time PCR analysis, when compared to Nip. These results imply that OsAKR1 controlled rice's ability to withstand Cd by scavenging harmful aldehydes and turning on the reactive oxygen species (ROS) scavenging mechanism.
Asunto(s)
Aldo-Ceto Reductasas , Cadmio , Oryza , Oryza/genética , Oryza/metabolismo , Oryza/efectos de los fármacos , Oryza/crecimiento & desarrollo , Cadmio/toxicidad , Cadmio/metabolismo , Aldo-Ceto Reductasas/genética , Aldo-Ceto Reductasas/metabolismo , Aldehídos/metabolismo , Catalasa/metabolismo , Catalasa/genética , Aldehído Reductasa/genética , Aldehído Reductasa/metabolismo , Malondialdehído/metabolismo , Estrés Fisiológico , Piruvaldehído/metabolismo , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Peróxido de Hidrógeno/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Mutación , Raíces de Plantas/metabolismo , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/genética , Inactivación MetabólicaRESUMEN
Various phosphate-solubilizing bacterial strains were isolated from the Hippochaete ramosissimum rhizosphere in Tongguanshan copper tailings in Tongling of Anhui Province, East China. After many times of screening and purification, a strain B25 with stronger phosphate-solubilizing capability was obtained, which belonged to Bacillus genus, as identified by transmission electron microscope and DNA molecular approaches. A culture experiment was conducted to study the phosphate-solubilizing capability of the B25 within 168 h and the variations of the medium pH and B25 growth as well as the phosphate-solubilizing capability of B25 under different culture conditions. A weak correlation was observed between the phosphate-solubilizing capability of B25 and the medium pH. The B25 displayed a better phosphate-solubilizing capability when the carbon source was glucose, medium initial pH was 7.0, and culture temperature was 30 degrees C.
Asunto(s)
Bacillus/aislamiento & purificación , Bacillus/metabolismo , Fosfatos/metabolismo , Microbiología del Suelo , Bacillus/crecimiento & desarrollo , Productos Agrícolas/crecimiento & desarrollo , Fertilizantes , Fosfatos/química , Rizosfera , Suelo/análisis , SolubilidadRESUMEN
In this work, we investigated the anaerobic decolorization of methyl orange (MO), a typical azo dye, by Shewanella oneidensis MR-1, which can use various organic and inorganic substances as its electron acceptor in natural and engineered environments. S. oneidensis MR-1 was found to be able to obtain energy for growth through anaerobic respiration accompanied with dissimilatory azo-reduction of MO. Chemical analysis shows that MO reduction occurred via the cleavage of azo bond. Block of Mtr respiratory pathway, a transmembrane electron transport chain, resulted in a reduction of decolorization rate by 80%, compared to the wild type. Knockout of cymA resulted in a substantial loss of its azo-reduction ability, indicating that CymA is a key c-type cytochrome in the electron transfer chain to MO. Thus, the MtrA-MtrB-MtrC respiratory pathway is proposed to be mainly responsible for the anaerobic decolorization of azo dyes such as MO by S. oneidensis.