RESUMEN
OBJECTIVE: To study the chemical constituents of Ajuga nipponensis. METHODS: The chemical constituents were isolated by repeated silica gel column chromatography and their structures were elucidated by phyisochemical properties and spectral analysis. RESULTS: Ten compounds were isolated and identified as:hexadecanoic acid(1), ajuforrestin A(2), beta-sitosterol(3), acacetin(4), apigenin(5), ajugamacrin B(6), ursolic acid(7), beta-ecdysone(8), 8-acetylharpagide(9) and daucosterol(10). CONCLUSION: Compounds 1-7 and 10 are isolated from this plant for the first time.
Asunto(s)
Ajuga/química , Apigenina/química , Ácido Palmítico/química , Plantas Medicinales/química , Apigenina/aislamiento & purificación , Flavonas/química , Flavonas/aislamiento & purificación , Estructura Molecular , Ácido Palmítico/aislamiento & purificación , Sitoesteroles/química , Sitoesteroles/aislamiento & purificación , Espectrofotometría UltravioletaRESUMEN
Total saponin of Solanum lyratum Thunb (TSSLT), a species of natural biologically active substances isolated from Solanum lyratum Thunb, possesses various bioactivities. It has been proposed that the induction of apoptosis may be the basis of its antitumor activity. However, the molecular mechanism underlying the total saponin-induced apoptotic process remains unknown. In the present study, we describe the anti-proliferative effect of TSSLT on human cervical cancer cells (Hela). The TSSLT induced apoptosis of Hela in a time-dependent manner with an IC50 for cell viability of 6 microg/ml. The TSSLT-induced cell death was characterized by changes in cell morphology, DNA fragmentation, activation of caspase-like activities, poly (ADP-ribose) polymerase (PARP) cleavage and release of cytochrome c (cyt c) into cytosol. TSSLT activated various caspases such as caspase-3, -8, and -9 (like) activities but not caspase-1 like activity. The cell death was completely prevented by the pan caspase inhibitor benzyloxy carbonyl-Val-Ala-Asp- fluoromethyl-ketone (Z-VAD-FMK). More than 80% cell survival was observed in the presence of a caspase-3 inhibitor. In addition, treatment with TSSLT induced the increase of Bax:Bcl-2 ratio in Hela cells. These results suggest that the induction of apoptosis by TSSLT involves multiple pathways antigen including death receptor and mitochondrial pathway and strongly suggest that the mitochondrial pathway was mediated by low expression of Bcl-2 and upregulation of Bax, release of cyt c and subsequent activation of caspase-3 followed by down stream events leading to apoptotic cell death.
Asunto(s)
Antineoplásicos Fitogénicos , Apoptosis/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Saponinas/farmacología , Solanum/química , Anexina A5/metabolismo , Western Blotting , Caspasas/metabolismo , Muerte Celular/efectos de los fármacos , Citocromos c/metabolismo , Fragmentación del ADN/efectos de los fármacos , Citometría de Flujo , Células HeLa , Humanos , Poli(ADP-Ribosa) Polimerasas/metabolismo , Proteínas Proto-Oncogénicas c-bcl-2/metabolismo , Transducción de Señal/efectos de los fármacos , Sales de Tetrazolio , Tiazoles , Proteína X Asociada a bcl-2/metabolismoRESUMEN
A novel alkaloid, hupcrispatine (1), has been isolated from the unique Chinese species Huperzia crispate Ching. The structure of hupcrispatine has been elucidated as 9-amino-6-methyl-3-quinolone on the basis of spectral evidence.
Asunto(s)
Alcaloides/química , Huperzia/química , Quinolinas/química , Estructura MolecularRESUMEN
OBJECTIVE: To study the effects of Dihydromyricetin (DMY) on antilipid-peroxidation. METHOD: The antilipid-peroxidation of DMY on heart, liver, brain tissue homogenate and mitochondria was measured by the determination of malondiadehyde (MDA) induced by Fe2+ -Vit C, Fe2+ -H2O2, Fe-Cys with TBA spectrometric method. RESULT: DMY could inhibit the lipid peroxidation of homogenate and mitochondria. The inhibition exhibited concentration-dependent manner. CONCLUSION: DMY has good antilipid-peroxidation effect, which is worth studing further.