RESUMEN
Selected oligomers representing the high, medium, and low molecular weight fractions of the spermicidal agent Nonoxynol-9 (N-9) were separated by HPLC. Nonoxynol-9 and the isolated fractions were formulated with polyvinylpyrrolidone (PVP) in order to increase their water solubility, particularly that of the insoluble low molecular weight fraction. The in vitro spermicidal activity of three molecular weight fractions were compared to that of N-9, using rabbit spermatozoa, at equimolar concentrations. Nonoxynol-9/PVP was far more effective in immobilizing the sperm than either N-9 alone or the separate fractions. The relative spermicidal activity of the oligomers was of the order middle molecular weight greater than high molecular weight greater than low molecular weight.
Asunto(s)
Polietilenglicoles/farmacología , Espermicidas/farmacología , Espermatozoides/efectos de los fármacos , Animales , Cromatografía Líquida de Alta Presión , Masculino , Peso Molecular , Nonoxinol , Polietilenglicoles/aislamiento & purificación , Conejos , Solubilidad , Motilidad Espermática/efectos de los fármacosRESUMEN
A simple reverse-phase liquid chromatographic system with ultraviolet detection at 254 nm for tetrafluoroputrescine (TFP), a putrescine analog, is described. The assay involves precolumn derivatization of TFP with dansyl chloride at pH 6.8-7.5 at 60 degrees C, followed by separation from putrescine (PUT) and quantitation. The derivatization procedure was adapted for the simultaneous analysis of TFP and PUT in whole blood components. Also, preliminary studies on protein binding of TFP are reported.
Asunto(s)
Unión Proteica , Putrescina/análogos & derivados , Animales , Cromatografía Líquida de Alta Presión , Compuestos de Dansilo/sangre , Eritrocitos/análisis , Concentración de Iones de Hidrógeno , Cinética , Plasma/análisis , Putrescina/análisis , Putrescina/sangre , Ratas , Albúmina Sérica Bovina , Espectrofotometría Ultravioleta , TemperaturaRESUMEN
Fluorine-19 nuclear magnetic resonance spectroscopy is used to detect the in vitro uptake of tetrafluoroputrescine, a novel putrescine analogue, in red blood cells.